Stable isotope probing: methods and protocols
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionatio...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
2046 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study |
| Beschreibung: | Experimental Set Up and Data Analysis Considerations for DNA- and RNA-SIP Experiments in the Omics Era -- DNA-Based Stable Isotope Probing -- RNA Stable Isotope Probing (RNA-SIP) -- Stable Isotope Probing of Microbial Phospholipid Fatty Acids in Environmental Samples -- SIP-Metaproteomics: Linking Microbial Taxonomy, Function, and Activity -- Chip-SIP: Stable Isotope Probing Analyzed with rRNA-Targeted Microarrays and NanoSIMS -- Quantification of Methanogenic Pathways Using Stable Carbon Isotopic Signatures -- Stable Isotope-Labeled Single-Cell Raman Spectroscopy Revealing Function and Activity of Environmental Microbes -- Data Analysis for DNA Stable Isotope Probing Experiments Using Multiple Window High Resolution SIP -- Stable Isotope Probing of Microorganisms in Environmental Samples with H218O -- Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing -- Profiling of Active Microorganisms by Stable Isotope Probing-Metagenomics -- Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing -- Stable Isotope Probing Techniques and Methodological Considerations Using 15N -- DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea -- Method Development for DNA and Proteome SIP Analysis of Activated Sludge for Anaerobic Dichloromethane Biodegradation -- RNA-Based Stable Isotope Probing (RNA-SIP) in the Gut Environment -- Stable Isotope Probing of Microbiota Structure and Function in the Plant Rhizosphere |
| Beschreibung: | 1 Online-Ressource (XI, 247 Seiten) Illustrationen |
| ISBN: | 9781493997213 |
| DOI: | 10.1007/978-1-4939-9721-3 |
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| 520 | |a This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study | ||
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| spelling | Stable isotope probing methods and protocols edited by Marc G. Dumont, Marcela Hernández García 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XI, 247 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2046 Experimental Set Up and Data Analysis Considerations for DNA- and RNA-SIP Experiments in the Omics Era -- DNA-Based Stable Isotope Probing -- RNA Stable Isotope Probing (RNA-SIP) -- Stable Isotope Probing of Microbial Phospholipid Fatty Acids in Environmental Samples -- SIP-Metaproteomics: Linking Microbial Taxonomy, Function, and Activity -- Chip-SIP: Stable Isotope Probing Analyzed with rRNA-Targeted Microarrays and NanoSIMS -- Quantification of Methanogenic Pathways Using Stable Carbon Isotopic Signatures -- Stable Isotope-Labeled Single-Cell Raman Spectroscopy Revealing Function and Activity of Environmental Microbes -- Data Analysis for DNA Stable Isotope Probing Experiments Using Multiple Window High Resolution SIP -- Stable Isotope Probing of Microorganisms in Environmental Samples with H218O -- Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing -- Profiling of Active Microorganisms by Stable Isotope Probing-Metagenomics -- Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing -- Stable Isotope Probing Techniques and Methodological Considerations Using 15N -- DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea -- Method Development for DNA and Proteome SIP Analysis of Activated Sludge for Anaerobic Dichloromethane Biodegradation -- RNA-Based Stable Isotope Probing (RNA-SIP) in the Gut Environment -- Stable Isotope Probing of Microbiota Structure and Function in the Plant Rhizosphere This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study Microbiology Microbial genetics Microbial genomics Dumont, Marc G. (DE-588)1194011071 edt Hernández García, Marcela (DE-588)1194011004 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9723-7 https://doi.org/10.1007/978-1-4939-9721-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Stable isotope probing methods and protocols Microbiology Microbial genetics Microbial genomics |
| title | Stable isotope probing methods and protocols |
| title_auth | Stable isotope probing methods and protocols |
| title_exact_search | Stable isotope probing methods and protocols |
| title_exact_search_txtP | Stable isotope probing methods and protocols |
| title_full | Stable isotope probing methods and protocols edited by Marc G. Dumont, Marcela Hernández García |
| title_fullStr | Stable isotope probing methods and protocols edited by Marc G. Dumont, Marcela Hernández García |
| title_full_unstemmed | Stable isotope probing methods and protocols edited by Marc G. Dumont, Marcela Hernández García |
| title_short | Stable isotope probing |
| title_sort | stable isotope probing methods and protocols |
| title_sub | methods and protocols |
| topic | Microbiology Microbial genetics Microbial genomics |
| topic_facet | Microbiology Microbial genetics Microbial genomics |
| url | https://doi.org/10.1007/978-1-4939-9721-3 |
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