Neuroproteomics:
This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accura...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 2nd ed. 2019 |
| Schriftenreihe: | Neuromethods
146 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms |
| Beschreibung: | Neuroproteomics: The Methods -- Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue -- Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice -- Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue -- High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics -- Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3) -- Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip -- Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes -- Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry -- TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons -- Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures -- Quantitative Analysis of Mass Spectrometry-Based Proteomics Data -- 2D-DIGE as a Tool in Neuroproteomics -- Neuropeptidomics of the Mammalian Brain -- MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides -- A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder |
| Beschreibung: | 1 Online-Ressource (XIII, 218 Seiten) Illustrationen |
| ISBN: | 9781493996629 |
| DOI: | 10.1007/978-1-4939-9662-9 |
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| 520 | |a This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms | ||
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| dewey-full | 612.8 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
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| discipline | Medizin |
| discipline_str_mv | Medizin |
| doi_str_mv | 10.1007/978-1-4939-9662-9 |
| edition | 2nd ed. 2019 |
| format | Electronic eBook |
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| id | DE-604.BV047645308 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:48:37Z |
| indexdate | 2024-07-10T09:18:09Z |
| institution | BVB |
| isbn | 9781493996629 |
| language | English |
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| spelling | Neuroproteomics edited by Ka Wan Li 2nd ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XIII, 218 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Neuromethods 146 Neuroproteomics: The Methods -- Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue -- Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice -- Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue -- High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics -- Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3) -- Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip -- Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes -- Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry -- TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons -- Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures -- Quantitative Analysis of Mass Spectrometry-Based Proteomics Data -- 2D-DIGE as a Tool in Neuroproteomics -- Neuropeptidomics of the Mammalian Brain -- MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides -- A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms Neurosciences Proteomics Li, Ka Wan (DE-588)1196044066 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9664-3 https://doi.org/10.1007/978-1-4939-9662-9 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Neuroproteomics Neurosciences Proteomics |
| title | Neuroproteomics |
| title_auth | Neuroproteomics |
| title_exact_search | Neuroproteomics |
| title_exact_search_txtP | Neuroproteomics |
| title_full | Neuroproteomics edited by Ka Wan Li |
| title_fullStr | Neuroproteomics edited by Ka Wan Li |
| title_full_unstemmed | Neuroproteomics edited by Ka Wan Li |
| title_short | Neuroproteomics |
| title_sort | neuroproteomics |
| topic | Neurosciences Proteomics |
| topic_facet | Neurosciences Proteomics |
| url | https://doi.org/10.1007/978-1-4939-9662-9 |
| work_keys_str_mv | AT likawan neuroproteomics |