Chlamydia trachomatis: methods and protocols
This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequenc...
Saved in:
| Other Authors: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY
Springer New York
2019
|
| Edition: | 1st ed. 2019 |
| Series: | Methods in Molecular Biology
2042 |
| Subjects: | |
| Online Access: | UBR01 TUM01 Volltext |
| Summary: | This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs |
| Item Description: | Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA) -- Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue -- Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae -- Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay -- Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth -- Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples -- Multi Locus Sequence Typing (MLST) of Chlamydiales -- Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method -- Dual RNA-Seq of Chlamydia and Host Cells -- Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection -- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions -- Mutagenesis of Chlamydia trachomatis Using TargeTron -- Forward and Reverse Genetic Analysis of Chlamydia -- A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions -- Rodent Infections for Chlamydia spp. -- Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy -- Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane -- High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis |
| Physical Description: | 1 Online-Ressource (XI, 289 Seiten) Illustrationen |
| ISBN: | 9781493996940 |
| DOI: | 10.1007/978-1-4939-9694-0 |
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| spelling | Chlamydia trachomatis methods and protocols edited by Amanda Claire Brown 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XI, 289 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2042 Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA) -- Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue -- Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae -- Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay -- Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth -- Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples -- Multi Locus Sequence Typing (MLST) of Chlamydiales -- Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method -- Dual RNA-Seq of Chlamydia and Host Cells -- Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection -- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions -- Mutagenesis of Chlamydia trachomatis Using TargeTron -- Forward and Reverse Genetic Analysis of Chlamydia -- A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions -- Rodent Infections for Chlamydia spp. -- Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy -- Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane -- High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs Medical microbiology Infectious diseases Pharmaceutical technology Bacteriology Chlamydia trachomatis (DE-588)4192858-1 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Chlamydia trachomatis (DE-588)4192858-1 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Brown, Amanda Claire ca. 20./21. Jhr. (DE-588)1229856404 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9696-4 https://doi.org/10.1007/978-1-4939-9694-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Chlamydia trachomatis methods and protocols Medical microbiology Infectious diseases Pharmaceutical technology Bacteriology Chlamydia trachomatis (DE-588)4192858-1 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4192858-1 (DE-588)4038971-6 (DE-588)4039983-7 (DE-588)4143413-4 |
| title | Chlamydia trachomatis methods and protocols |
| title_auth | Chlamydia trachomatis methods and protocols |
| title_exact_search | Chlamydia trachomatis methods and protocols |
| title_exact_search_txtP | Chlamydia trachomatis methods and protocols |
| title_full | Chlamydia trachomatis methods and protocols edited by Amanda Claire Brown |
| title_fullStr | Chlamydia trachomatis methods and protocols edited by Amanda Claire Brown |
| title_full_unstemmed | Chlamydia trachomatis methods and protocols edited by Amanda Claire Brown |
| title_short | Chlamydia trachomatis |
| title_sort | chlamydia trachomatis methods and protocols |
| title_sub | methods and protocols |
| topic | Medical microbiology Infectious diseases Pharmaceutical technology Bacteriology Chlamydia trachomatis (DE-588)4192858-1 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Medical microbiology Infectious diseases Pharmaceutical technology Bacteriology Chlamydia trachomatis Methode Molekularbiologie Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-9694-0 |
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