This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequenc...

Ausführliche Beschreibung

Bibliographische Detailangaben

Weitere Verfasser:	Brown, Amanda Claire ca. 20./21. Jhr (HerausgeberIn)
Format:	Elektronisch E-Book
Sprache:	English
Veröffentlicht:	New York, NY Springer New York 2019
Ausgabe:	1st ed. 2019
Schriftenreihe:	Methods in Molecular Biology 2042
Schlagworte:	Medical microbiology Infectious diseases Pharmaceutical technology Bacteriology Chlamydia trachomatis Methode Molekularbiologie Aufsatzsammlung
Online-Zugang:	UBR01 TUM01 Volltext
Zusammenfassung:	This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs
Beschreibung:	Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA) -- Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue -- Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae -- Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay -- Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth -- Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples -- Multi Locus Sequence Typing (MLST) of Chlamydiales -- Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method -- Dual RNA-Seq of Chlamydia and Host Cells -- Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection -- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions -- Mutagenesis of Chlamydia trachomatis Using TargeTron -- Forward and Reverse Genetic Analysis of Chlamydia -- A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions -- Rodent Infections for Chlamydia spp. -- Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy -- Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane -- High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis
Beschreibung:	1 Online-Ressource (XI, 289 Seiten) Illustrationen
ISBN:	9781493996940
DOI:	10.1007/978-1-4939-9694-0