Radiation cytogenetics: methods and protocols
This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA dam...
Gespeichert in:
| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1984 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory |
| Beschreibung: | Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis -- Animal Lymphocyte Metaphase Chromosome Preparation -- Micronuclei Formation Analysis after Ionizing Radiation -- G1 Premature Chromosome Condensation (PCC) Assay -- G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity -- G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology -- Sister Chromatid Exchange as a Genotoxic Stress Marker -- DNA Damage Focus Formation Assay -- Nuclear Foci Assays in Live Cells -- In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes -- PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations -- Modified PNA Telomere and Centromere FISH Protocols -- Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements -- Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH -- Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting |
| Beschreibung: | 1 Online-Ressource (X, 138 Seiten) Illustrationen |
| ISBN: | 9781493994328 |
| DOI: | 10.1007/978-1-4939-9432-8 |
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| dewey-full | 599.935 611.01816 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 599 - Mammalia 611 - Human anatomy, cytology, histology |
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| discipline | Biologie Medizin |
| discipline_str_mv | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-4939-9432-8 |
| edition | 1st ed. 2019 |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:48:36Z |
| indexdate | 2024-07-10T09:18:09Z |
| institution | BVB |
| isbn | 9781493994328 |
| language | English |
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| spelling | Radiation cytogenetics methods and protocols edited by Takamitsu A. Kato, Paul F. Wilson 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (X, 138 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1984 Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis -- Animal Lymphocyte Metaphase Chromosome Preparation -- Micronuclei Formation Analysis after Ionizing Radiation -- G1 Premature Chromosome Condensation (PCC) Assay -- G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity -- G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology -- Sister Chromatid Exchange as a Genotoxic Stress Marker -- DNA Damage Focus Formation Assay -- Nuclear Foci Assays in Live Cells -- In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes -- PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations -- Modified PNA Telomere and Centromere FISH Protocols -- Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements -- Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH -- Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory Human genetics Cell culture Kato, Takamitsu ca. 20./21. Jh. (DE-588)1247768430 edt Wilson, Paul F. ca. 20./21. Jh. (DE-588)1247768686 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9431-1 https://doi.org/10.1007/978-1-4939-9432-8 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Radiation cytogenetics methods and protocols Human genetics Cell culture |
| title | Radiation cytogenetics methods and protocols |
| title_auth | Radiation cytogenetics methods and protocols |
| title_exact_search | Radiation cytogenetics methods and protocols |
| title_exact_search_txtP | Radiation cytogenetics methods and protocols |
| title_full | Radiation cytogenetics methods and protocols edited by Takamitsu A. Kato, Paul F. Wilson |
| title_fullStr | Radiation cytogenetics methods and protocols edited by Takamitsu A. Kato, Paul F. Wilson |
| title_full_unstemmed | Radiation cytogenetics methods and protocols edited by Takamitsu A. Kato, Paul F. Wilson |
| title_short | Radiation cytogenetics |
| title_sort | radiation cytogenetics methods and protocols |
| title_sub | methods and protocols |
| topic | Human genetics Cell culture |
| topic_facet | Human genetics Cell culture |
| url | https://doi.org/10.1007/978-1-4939-9432-8 |
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