Metabolomics: methods and protocols
The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier An...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1996 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson's Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested in this exciting and evolving field. |
| Beschreibung: | Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer -- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis -- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals -- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA) with Internal Standards -- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods -- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods -- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS -- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates -- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System -- - Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM -- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS) -- Analysis of Microbial Siderophores by Mass Spectrometry -- HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer -- Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification -- Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites -- Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells -- Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes -- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis -- Protocols for Metabolomic Analyses from Tissues in Parkinson's Disease -- - Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease -- Proteometabolomics of Melphalan Resistance in Multiple Myeloma -- Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading -- Protocols for NMR Analysis in Livestock Metabolomics -- Metabolomics Applicable to Retinal Vascular Diseases |
| Beschreibung: | 1 Online-Ressource (XV, 335 Seiten) Illustrationen |
| ISBN: | 9781493994885 |
| DOI: | 10.1007/978-1-4939-9488-5 |
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| spelling | Metabolomics methods and protocols edited by Sanjoy K. Bhattacharya 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XV, 335 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1996 Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer -- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis -- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals -- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA) with Internal Standards -- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods -- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods -- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS -- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates -- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System -- - Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM -- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS) -- Analysis of Microbial Siderophores by Mass Spectrometry -- HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer -- Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification -- Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites -- Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells -- Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes -- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis -- Protocols for Metabolomic Analyses from Tissues in Parkinson's Disease -- - Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease -- Proteometabolomics of Melphalan Resistance in Multiple Myeloma -- Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading -- Protocols for NMR Analysis in Livestock Metabolomics -- Metabolomics Applicable to Retinal Vascular Diseases The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson's Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested in this exciting and evolving field. Metabolism Mass spectrometry Bhattacharya, Sanjoy ca. 20./21. Jh. (DE-588)1247737721 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9490-8 https://doi.org/10.1007/978-1-4939-9488-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Metabolomics methods and protocols Metabolism Mass spectrometry |
| title | Metabolomics methods and protocols |
| title_auth | Metabolomics methods and protocols |
| title_exact_search | Metabolomics methods and protocols |
| title_exact_search_txtP | Metabolomics methods and protocols |
| title_full | Metabolomics methods and protocols edited by Sanjoy K. Bhattacharya |
| title_fullStr | Metabolomics methods and protocols edited by Sanjoy K. Bhattacharya |
| title_full_unstemmed | Metabolomics methods and protocols edited by Sanjoy K. Bhattacharya |
| title_short | Metabolomics |
| title_sort | metabolomics methods and protocols |
| title_sub | methods and protocols |
| topic | Metabolism Mass spectrometry |
| topic_facet | Metabolism Mass spectrometry |
| url | https://doi.org/10.1007/978-1-4939-9488-5 |
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