High-throughput metabolomics: methods and protocols
This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracer...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1978 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine |
| Beschreibung: | Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells -- High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods -- High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research -- Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications -- CE-MS for Metabolomics: A Comparison with Other Techniques -- Overview of Lipid Mass Spectrometry and Lipidomics -- LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins -- Untargeted and Semi-Targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics -- HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle -- Quantification of D- and L-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry -- - Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples -- Untargeted Metabolomics of Plant Leaf Tissues -- Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling -- Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry -- Insights into Dynamic Network States Using Metabolomic Data -- Analysis of Endothelial Fatty Acid Metabolism Using Tracer Metabolomics -- Using Stable Isotope Tracers for Metabolic Pathway Analysis -- Data Processing for GC-MS and LC-MS-Based Untargeted Metabolomics -- El-MAVEN: A Fast, Robust, and User-Friendly Mass Spectrometry Data Processing Engine for Metabolomics -- Pre-Analytic Considerations for Mass Spectrometry-Based Untargeted Metabolomics Data -- Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically-Encoded Biosensors -- - Microplate Assays for Spectrophotometric Measurement of Mitochondrial Enzyme Activity -- Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy -- Metabolomic Applications in Radiation Biodosimetry -- Metabolomics Analyses to Investigate the Role of Diet and Physical Training -- Blood Biomarkers in Sports Medicine and Performance and the Future of Metabolomics -- Personalized Metabolomics |
| Beschreibung: | 1 Online-Ressource (XV, 459 Seiten) Illustrationen |
| ISBN: | 9781493992362 |
| DOI: | 10.1007/978-1-4939-9236-2 |
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| 520 | |a This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine | ||
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| edition | 1st ed. 2019 |
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| isbn | 9781493992362 |
| language | English |
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| spelling | High-throughput metabolomics methods and protocols edited by Angelo D'Alessandro 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XV, 459 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1978 Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells -- High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods -- High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research -- Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications -- CE-MS for Metabolomics: A Comparison with Other Techniques -- Overview of Lipid Mass Spectrometry and Lipidomics -- LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins -- Untargeted and Semi-Targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics -- HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle -- Quantification of D- and L-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry -- - Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples -- Untargeted Metabolomics of Plant Leaf Tissues -- Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling -- Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry -- Insights into Dynamic Network States Using Metabolomic Data -- Analysis of Endothelial Fatty Acid Metabolism Using Tracer Metabolomics -- Using Stable Isotope Tracers for Metabolic Pathway Analysis -- Data Processing for GC-MS and LC-MS-Based Untargeted Metabolomics -- El-MAVEN: A Fast, Robust, and User-Friendly Mass Spectrometry Data Processing Engine for Metabolomics -- Pre-Analytic Considerations for Mass Spectrometry-Based Untargeted Metabolomics Data -- Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically-Encoded Biosensors -- - Microplate Assays for Spectrophotometric Measurement of Mitochondrial Enzyme Activity -- Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy -- Metabolomic Applications in Radiation Biodosimetry -- Metabolomics Analyses to Investigate the Role of Diet and Physical Training -- Blood Biomarkers in Sports Medicine and Performance and the Future of Metabolomics -- Personalized Metabolomics This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine Metabolism Human genetics Lipids Bioinformatics D'Alessandro, Angelo ca. 20./21. Jh. (DE-588)1247735931 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9238-6 https://doi.org/10.1007/978-1-4939-9236-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | High-throughput metabolomics methods and protocols Metabolism Human genetics Lipids Bioinformatics |
| title | High-throughput metabolomics methods and protocols |
| title_auth | High-throughput metabolomics methods and protocols |
| title_exact_search | High-throughput metabolomics methods and protocols |
| title_exact_search_txtP | High-throughput metabolomics methods and protocols |
| title_full | High-throughput metabolomics methods and protocols edited by Angelo D'Alessandro |
| title_fullStr | High-throughput metabolomics methods and protocols edited by Angelo D'Alessandro |
| title_full_unstemmed | High-throughput metabolomics methods and protocols edited by Angelo D'Alessandro |
| title_short | High-throughput metabolomics |
| title_sort | high throughput metabolomics methods and protocols |
| title_sub | methods and protocols |
| topic | Metabolism Human genetics Lipids Bioinformatics |
| topic_facet | Metabolism Human genetics Lipids Bioinformatics |
| url | https://doi.org/10.1007/978-1-4939-9236-2 |
| work_keys_str_mv | AT dalessandroangelo highthroughputmetabolomicsmethodsandprotocols |