Developmental toxicology: methods and protocols
This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both sta...
Gespeichert in:
| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 2nd ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1965 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab. Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Edition serves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures |
| Beschreibung: | In Vitro Models in Developmental Toxicology -- Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments -- Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology -- Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model -- Micromass Methods for the Evaluation of Developmental Toxicants -- The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System -- Embryonic Mid-Facial Palatal Organ Culture Methods in Developmental Toxicology -- Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology -- Hepatotoxicity in Zebrafish Larvae -- Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model -- The Chicken Embryo as a Model in Developmental Toxicology -- The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity -- - Mouse Whole Embryo Culture -- Rat Whole Embryo Culture -- Rabbit Whole Embryo Culture -- Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology -- Identifying Toxicant-Interacting Genes Using Forward-Genetic Screening in Zebrafish -- Assessment of Histiotrophic Nutrition Using Fluorescent Probes -- Examining Gene Expression Patterns through Whole Mount In Situ Hybridization -- Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos -- Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection -- Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA -- Western Analysis of Breast Cancer 1 Protein (BRCA1) -- Regulation and Control of AP-1 Binding Activity in Embryotoxicity -- - Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR) -- The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity -- Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining |
| Beschreibung: | 1 Online-Ressource (XI, 436 Seiten) Illustrationen |
| ISBN: | 9781493991822 |
| DOI: | 10.1007/978-1-4939-9182-2 |
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| edition | 2nd ed. 2019 |
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| spelling | Developmental toxicology methods and protocols edited by Jason M. Hansen, Louise M. Winn 2nd ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XI, 436 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1965 In Vitro Models in Developmental Toxicology -- Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments -- Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology -- Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model -- Micromass Methods for the Evaluation of Developmental Toxicants -- The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System -- Embryonic Mid-Facial Palatal Organ Culture Methods in Developmental Toxicology -- Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology -- Hepatotoxicity in Zebrafish Larvae -- Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model -- The Chicken Embryo as a Model in Developmental Toxicology -- The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity -- - Mouse Whole Embryo Culture -- Rat Whole Embryo Culture -- Rabbit Whole Embryo Culture -- Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology -- Identifying Toxicant-Interacting Genes Using Forward-Genetic Screening in Zebrafish -- Assessment of Histiotrophic Nutrition Using Fluorescent Probes -- Examining Gene Expression Patterns through Whole Mount In Situ Hybridization -- Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos -- Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection -- Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA -- Western Analysis of Breast Cancer 1 Protein (BRCA1) -- Regulation and Control of AP-1 Binding Activity in Embryotoxicity -- - Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR) -- The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity -- Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab. Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Edition serves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures Pharmacology Embryology Cell biology Hansen, Jason M. (DE-588)1187292354 edt Winn, Louise M. (DE-588)1187292168 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9183-9 https://doi.org/10.1007/978-1-4939-9182-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Developmental toxicology methods and protocols Pharmacology Embryology Cell biology |
| title | Developmental toxicology methods and protocols |
| title_auth | Developmental toxicology methods and protocols |
| title_exact_search | Developmental toxicology methods and protocols |
| title_exact_search_txtP | Developmental toxicology methods and protocols |
| title_full | Developmental toxicology methods and protocols edited by Jason M. Hansen, Louise M. Winn |
| title_fullStr | Developmental toxicology methods and protocols edited by Jason M. Hansen, Louise M. Winn |
| title_full_unstemmed | Developmental toxicology methods and protocols edited by Jason M. Hansen, Louise M. Winn |
| title_short | Developmental toxicology |
| title_sort | developmental toxicology methods and protocols |
| title_sub | methods and protocols |
| topic | Pharmacology Embryology Cell biology |
| topic_facet | Pharmacology Embryology Cell biology |
| url | https://doi.org/10.1007/978-1-4939-9182-2 |
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