Leishmania: methods and protocols
This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1971 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide. |
| Beschreibung: | Host-Free Systems for Differentiation of Axenic Leishmania -- Phylogenetic Studies -- A Guide to Next Generation Sequence Analysis of Leishmania Genomes -- Quantitative RNA Analysis using RNA Seq -- Ribosome Profiling in Trypanosomatids -- Cosmid Library Construction and Functional Cloning -- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania -- Gene Replacement by Homologous Recombination -- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9 -- DiCre-Based Inducible Disruption of Leishmania Genes -- DiCre-Based Inducible Gene Expression -- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments -- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR) -- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening -- Quantification of Parasite Loads by Automated Microscopic Image Analysis -- Quantification of Leishmania Parasites in Murine Models of Visceral Infection -- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis -- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease -- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp |
| Beschreibung: | 1 Online-Ressource (XIII, 370 Seiten) Illustrationen |
| ISBN: | 9781493992102 |
| DOI: | 10.1007/978-1-4939-9210-2 |
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| 520 | |a This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide. | ||
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| dewey-ones | 616 - Diseases |
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| discipline | Medizin |
| discipline_str_mv | Medizin |
| doi_str_mv | 10.1007/978-1-4939-9210-2 |
| edition | 1st ed. 2019 |
| format | Electronic eBook |
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| isbn | 9781493992102 |
| language | English |
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| spelling | Leishmania methods and protocols edited by Joachim Clos 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XIII, 370 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1971 Host-Free Systems for Differentiation of Axenic Leishmania -- Phylogenetic Studies -- A Guide to Next Generation Sequence Analysis of Leishmania Genomes -- Quantitative RNA Analysis using RNA Seq -- Ribosome Profiling in Trypanosomatids -- Cosmid Library Construction and Functional Cloning -- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania -- Gene Replacement by Homologous Recombination -- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9 -- DiCre-Based Inducible Disruption of Leishmania Genes -- DiCre-Based Inducible Gene Expression -- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments -- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR) -- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening -- Quantification of Parasite Loads by Automated Microscopic Image Analysis -- Quantification of Leishmania Parasites in Murine Models of Visceral Infection -- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis -- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease -- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide. Infectious diseases Clos, Joachim ca. 20./21. Jh. (DE-588)1247588629 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9211-9 https://doi.org/10.1007/978-1-4939-9210-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Leishmania methods and protocols Infectious diseases |
| title | Leishmania methods and protocols |
| title_auth | Leishmania methods and protocols |
| title_exact_search | Leishmania methods and protocols |
| title_exact_search_txtP | Leishmania methods and protocols |
| title_full | Leishmania methods and protocols edited by Joachim Clos |
| title_fullStr | Leishmania methods and protocols edited by Joachim Clos |
| title_full_unstemmed | Leishmania methods and protocols edited by Joachim Clos |
| title_short | Leishmania |
| title_sort | leishmania methods and protocols |
| title_sub | methods and protocols |
| topic | Infectious diseases |
| topic_facet | Infectious diseases |
| url | https://doi.org/10.1007/978-1-4939-9210-2 |
| work_keys_str_mv | AT closjoachim leishmaniamethodsandprotocols |