Alpha-synuclein: methods and protocols
This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1948 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects |
| Beschreibung: | Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons -- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue -- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons -- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform -- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils -- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection -- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers -- Studying α-Synuclein Conformation by Intact-Cell Crosslinking -- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques -- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein -- Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes -- Yeast-Based Screens to Target Alpha-Synuclein Toxicity -- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy -- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers -- Monitoring α-Synuclein Proteotoxicity in Drosophila Models -- Detection of α-Synuclein in Biological Samples using Mass Spectrometry -- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation -- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons -- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy -- Expression and Purification of Untagged α-Synuclein -- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson's Disease |
| Beschreibung: | 1 Online-Ressource (XII, 289 Seiten) Illustrationen |
| ISBN: | 9781493991242 |
| DOI: | 10.1007/978-1-4939-9124-2 |
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| 520 | |a This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects | ||
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| spelling | Alpha-synuclein methods and protocols edited by Tim Bartels 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XII, 289 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1948 Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons -- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue -- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons -- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform -- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils -- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection -- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers -- Studying α-Synuclein Conformation by Intact-Cell Crosslinking -- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques -- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein -- Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes -- Yeast-Based Screens to Target Alpha-Synuclein Toxicity -- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy -- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers -- Monitoring α-Synuclein Proteotoxicity in Drosophila Models -- Detection of α-Synuclein in Biological Samples using Mass Spectrometry -- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation -- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons -- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy -- Expression and Purification of Untagged α-Synuclein -- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson's Disease This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects Proteins Neurosciences Bartels, Tim 1980- (DE-588)136092934 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9125-9 https://doi.org/10.1007/978-1-4939-9124-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Alpha-synuclein methods and protocols Proteins Neurosciences |
| title | Alpha-synuclein methods and protocols |
| title_auth | Alpha-synuclein methods and protocols |
| title_exact_search | Alpha-synuclein methods and protocols |
| title_exact_search_txtP | Alpha-synuclein methods and protocols |
| title_full | Alpha-synuclein methods and protocols edited by Tim Bartels |
| title_fullStr | Alpha-synuclein methods and protocols edited by Tim Bartels |
| title_full_unstemmed | Alpha-synuclein methods and protocols edited by Tim Bartels |
| title_short | Alpha-synuclein |
| title_sort | alpha synuclein methods and protocols |
| title_sub | methods and protocols |
| topic | Proteins Neurosciences |
| topic_facet | Proteins Neurosciences |
| url | https://doi.org/10.1007/978-1-4939-9124-2 |
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