Calpain: methods and protocols
This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover to...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1915 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Calpain: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases |
| Beschreibung: | Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography -- Bacterial Expression and Purification of Calpains -- Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity -- FRET-Based Assays to Determine Calpain Activity -- In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer -- Determining Temporal and Spatial Expression of Calpains in Amphibians -- Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis -- Expression and Activity of Calpain A in Drosophila melanogaster -- Use of the β-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1) -- LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence -- CalCleaveMKL: A Tool for Calpain Cleavage Prediction -- Identification of Calpain-Activated protein Functions -- Production and Purification of Recombinant Calpastatin -- Isolation of Endogenous Calpastatin -- Immunoblotting for Calpastatin Expression -- Experimental Manipulation of Calpain Activity In Vitro -- In Vivo Calpain Knockdown using Delivery of siRNA -- In Vivo Expression of Mutant Calpains in the Eye using Lentivirus -- Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians -- Genetic Models of Calpain Deficiency and Ectopic Expression |
| Beschreibung: | 1 Online-Ressource (XII, 276 Seiten) Illustrationen |
| ISBN: | 9781493989881 |
| DOI: | 10.1007/978-1-4939-8988-1 |
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| edition | 1st ed. 2019 |
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| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:47:24Z |
| indexdate | 2024-07-10T09:17:55Z |
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| isbn | 9781493989881 |
| language | English |
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| spelling | Calpain methods and protocols edited by Jeannette S. Messer 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XII, 276 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1915 Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography -- Bacterial Expression and Purification of Calpains -- Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity -- FRET-Based Assays to Determine Calpain Activity -- In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer -- Determining Temporal and Spatial Expression of Calpains in Amphibians -- Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis -- Expression and Activity of Calpain A in Drosophila melanogaster -- Use of the β-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1) -- LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence -- CalCleaveMKL: A Tool for Calpain Cleavage Prediction -- Identification of Calpain-Activated protein Functions -- Production and Purification of Recombinant Calpastatin -- Isolation of Endogenous Calpastatin -- Immunoblotting for Calpastatin Expression -- Experimental Manipulation of Calpain Activity In Vitro -- In Vivo Calpain Knockdown using Delivery of siRNA -- In Vivo Expression of Mutant Calpains in the Eye using Lentivirus -- Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians -- Genetic Models of Calpain Deficiency and Ectopic Expression This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Calpain: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases Proteins Messer, Jeannette ca. 20./21. Jh. (DE-588)1247568830 edt Erscheint auch als Druck-Ausgabe 978-1-4939-8989-8 https://doi.org/10.1007/978-1-4939-8988-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Calpain methods and protocols Proteins |
| title | Calpain methods and protocols |
| title_auth | Calpain methods and protocols |
| title_exact_search | Calpain methods and protocols |
| title_exact_search_txtP | Calpain methods and protocols |
| title_full | Calpain methods and protocols edited by Jeannette S. Messer |
| title_fullStr | Calpain methods and protocols edited by Jeannette S. Messer |
| title_full_unstemmed | Calpain methods and protocols edited by Jeannette S. Messer |
| title_short | Calpain |
| title_sort | calpain methods and protocols |
| title_sub | methods and protocols |
| topic | Proteins |
| topic_facet | Proteins |
| url | https://doi.org/10.1007/978-1-4939-8988-1 |
| work_keys_str_mv | AT messerjeannette calpainmethodsandprotocols |