Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
Apprimus Verlag
2021
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Applied Microbiology
Volume 26 |
| Schlagworte: | |
| Online-Zugang: | kostenfrei Inhaltsverzeichnis |
| Beschreibung: | XV, 162 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 278 g |
| ISBN: | 9783985550074 3985550077 |
| DOI: | 10.18154/RWTH-2021-11458 |
Internformat
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| 245 | 1 | 0 | |a Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives |c Christian Carl Blesken |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Aachen |b Apprimus Verlag |c 2021 | |
| 300 | |a XV, 162 Seiten |b Illustrationen, Diagramme |c 21 cm x 14.8 cm, 278 g | ||
| 336 | |b txt |2 rdacontent | ||
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| 502 | |b Dissertation |c Rheinisch-Westfälische Technische Hochschule Aachen |d 2021 | ||
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Datensatz im Suchindex
| _version_ | 1804183082545184768 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
SUMMARY
........................................................................................................................................................
ILL
ZUSAMMENFASSUNG
........................................................................................................................................
V
LIST
OF
ABBREVIATIONS
...................................................................................................................................
VII
LIST
OF
FIGURES
................................................................................................................................................
XI
LIST
OF
TABLES
.................................................................................................................................................
XV
1
GENERAL
INTRODUCTION
..........................................................................................................................
3
1.1
THE
BIOECONOMY
-
INITIATING
BIOTECHNOLOGY
FOR
A
SUSTAINABLE
ECONOMY
............................................
3
1.2
THE
PRODUCTION,
CHARACTERIZATION,
AND
UTILIZATION
OF
BIOSURFACTANTS
...................................................
5
1.2.1
RLS
AND
DERIVATIVES
AS
VALUABLE
GOODS
FOR
THE
BIOECONOMY
..............................................
6
1.3
BIOREACTOR
SYSTEMS
FOR
RHAMNOLIPID
PRODUCTION
...............................................................................
12
1.4
FOAM
FRACTIONATION FOR
AN
INTEGRATED
RHAMNOLIPID
RECOVERY
............................................................
15
1.5
DOWNSTREAM
PROCESSING
OF
RHAMNOLIPIDS
AND
DERIVATIVES
...............................................................
17
1.6
ACHIEVING
A
RENEWABLE
KEROSENE
PRODUCTION
....................................................................................
18
1.7
SCOPE
AND
OUTLINE
OF
THIS
THESIS
.........................................................................................................
21
2
MATERIAL
AND
METHODS
.....................................................................................................................
27
2.1
BACTERIAL
STRAINS
..................................................................................................................................
27
2.2
MEDIA,
CARBON
SOURCES,
AND
ANTIBIOTICS
..............................................................................................
29
2.3
CULTIVATION
PROCEDURES
........................................................................................................................
31
2.3.1
SHAKE
CULTURES
.....................................................................................................................
31
2.3.2
BIOREACTOR
PROCESSES
..........................................................................................................
32
2.4
HAA
STABILITY
IN
A
TREATED
AND
NON-TREATED
CULTURE
BROTH
.................................................................
40
2.5
ANALYTICAL
METHODS
.............................................................................................................................
40
2.5.1
BACTERIAL
GROWTH
.................................................................................................................
40
2.5.2
ANALYSIS
OF
GLUCOSE
AND
INTERMEDIATES
..............................................................................
40
2.5.3
ANALYSIS
OF
RLS AND
HAAS
................................................................................................
41
2.5.4
ANALYSIS
OF
AMMONIUM
......................................................................................................
41
2.5.5
ANALYSIS
OF
PYOVERDINES
AND
IMPURITIES
............................................................................
42
2.5.6
MEASURING
BACTERIAL
FOAM
ADHESION
..................................................................................
42
2.5.7
MEASURING
CELL
SURFACE
HYDROPHOBICITY
.............................................................................
43
2.5.8
OPTICAL
ANALYSIS
..................................................................................................................
44
2.5.9
DATA
ANALYSIS
.......................................................................................................................
44
2.6
HAA
PURIFICATION
METHODS
................................................................................................................
47
2.6.1
FOAM
FRACTIONATION
IN
A
STAND-ALONE
FRACTIONATION
COLUMN
................................................
47
2.6.2
FILTRATION
SYSTEMS
...............................................................................................................
48
2.6.3
ADSORPTION
AND DESORPTION
WITH
ADSORBENTS
......................................................................
49
I
TABLE
OF
CONTENTS
2.7
SEPARATION
OF
CIO-CIO
CONGENERS
WITH
A
PREPARATIVE
HPLC
............................................................
53
3
RESULTS
.................................................................................................................................................
61
3.1
RL
AND
HAA
PRODUCTION
BY
RECOMBINANT
PSEUDOMONAS
TOGETHER
WITH
METHODS
FOR
ENHANCED
PRODUCT
SEPARATION
................................................................................................................................
61
3.1.1
ABSTRACT
................................................................................................................................
61
3.1.2
INTRODUCTION
..........................................................................................................................
61
3.1.3
RESULTS
..................................................................................................................................
63
3.1.4
DISCUSSION
............................................................................................................................
81
3.2
GENETIC
CELL
SURFACE
MODIFICATION
FOR
OPTIMIZED
FOAM
FRACTIONATION
...............................................
89
3.2.1
ABSTRACT
................................................................................................................................
89
3.2.2
INTRODUCTION
..........................................................................................................................
89
3.2.3
RESULTS
..................................................................................................................................
91
3.2.4
DISCUSSION
............................................................................................................................
99
3.3
UNCOUPLING
FOAM
FRACTIONATION
AND
FOAM
ADSORPTION
FOR
ENHANCED
BIOSURFACTANT
SYNTHESIS
AND
RECOVERY
.............................................................................................................................................
107
3.3.1
ABSTRACT
..............................................................................................................................
107
3.3.2
INTRODUCTION
........................................................................................................................
107
3.3.3
RESULTS
................................................................................................................................
109
3.3.4
DISCUSSION
..........................................................................................................................
118
4
GENERAL
DISCUSSION
AND
OUTLOOK
...................................................................................................127
4.1
P.
PUTIDA
K.T2440
AS
IDEAL
RL
AND
HAA
PRODUCTION
HOST
................................................................
127
4.2
BIOREACTOR
PROCESSES
FOR
RL
AND
HAA
PRODUCTION
BY
A
NON-PATHOGENIC
P.
PUTIDA
K.T2440
WITH
FOAM
FRACTIONATION
AS
PREFERRED
TECHNIQUE
FOR
INTEGRATED
PRODUCT
RECOVERY
..................................
128
4.3
CONVERSION
OF
HAA
INTO
BIOFUEL
BY
ELECTROCHEMICAL
OR
CHEMO-CATALYTIC
DEOXYGENATION
.............
130
4.4
CONCLUSION
AND
OUTLOOK
.....................................................................................................................
131
5
APPENDIX
..........................................................................................................................................
137
5.1
SEPARATION
OF
CIO-CIO
CONGENER
OF
RL
AND
HAA
VIA PREPARATIVE
HPLC
.......................................
137
6
REFERENCES
........................................................................................................................................
143
CURRICULUM
VITAE
..........................................................................................................................................
159
II
|
| adam_txt |
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
SUMMARY
.
ILL
ZUSAMMENFASSUNG
.
V
LIST
OF
ABBREVIATIONS
.
VII
LIST
OF
FIGURES
.
XI
LIST
OF
TABLES
.
XV
1
GENERAL
INTRODUCTION
.
3
1.1
THE
BIOECONOMY
-
INITIATING
BIOTECHNOLOGY
FOR
A
SUSTAINABLE
ECONOMY
.
3
1.2
THE
PRODUCTION,
CHARACTERIZATION,
AND
UTILIZATION
OF
BIOSURFACTANTS
.
5
1.2.1
RLS
AND
DERIVATIVES
AS
VALUABLE
GOODS
FOR
THE
BIOECONOMY
.
6
1.3
BIOREACTOR
SYSTEMS
FOR
RHAMNOLIPID
PRODUCTION
.
12
1.4
FOAM
FRACTIONATION FOR
AN
INTEGRATED
RHAMNOLIPID
RECOVERY
.
15
1.5
DOWNSTREAM
PROCESSING
OF
RHAMNOLIPIDS
AND
DERIVATIVES
.
17
1.6
ACHIEVING
A
RENEWABLE
KEROSENE
PRODUCTION
.
18
1.7
SCOPE
AND
OUTLINE
OF
THIS
THESIS
.
21
2
MATERIAL
AND
METHODS
.
27
2.1
BACTERIAL
STRAINS
.
27
2.2
MEDIA,
CARBON
SOURCES,
AND
ANTIBIOTICS
.
29
2.3
CULTIVATION
PROCEDURES
.
31
2.3.1
SHAKE
CULTURES
.
31
2.3.2
BIOREACTOR
PROCESSES
.
32
2.4
HAA
STABILITY
IN
A
TREATED
AND
NON-TREATED
CULTURE
BROTH
.
40
2.5
ANALYTICAL
METHODS
.
40
2.5.1
BACTERIAL
GROWTH
.
40
2.5.2
ANALYSIS
OF
GLUCOSE
AND
INTERMEDIATES
.
40
2.5.3
ANALYSIS
OF
RLS AND
HAAS
.
41
2.5.4
ANALYSIS
OF
AMMONIUM
.
41
2.5.5
ANALYSIS
OF
PYOVERDINES
AND
IMPURITIES
.
42
2.5.6
MEASURING
BACTERIAL
FOAM
ADHESION
.
42
2.5.7
MEASURING
CELL
SURFACE
HYDROPHOBICITY
.
43
2.5.8
OPTICAL
ANALYSIS
.
44
2.5.9
DATA
ANALYSIS
.
44
2.6
HAA
PURIFICATION
METHODS
.
47
2.6.1
FOAM
FRACTIONATION
IN
A
STAND-ALONE
FRACTIONATION
COLUMN
.
47
2.6.2
FILTRATION
SYSTEMS
.
48
2.6.3
ADSORPTION
AND DESORPTION
WITH
ADSORBENTS
.
49
I
TABLE
OF
CONTENTS
2.7
SEPARATION
OF
CIO-CIO
CONGENERS
WITH
A
PREPARATIVE
HPLC
.
53
3
RESULTS
.
61
3.1
RL
AND
HAA
PRODUCTION
BY
RECOMBINANT
PSEUDOMONAS
TOGETHER
WITH
METHODS
FOR
ENHANCED
PRODUCT
SEPARATION
.
61
3.1.1
ABSTRACT
.
61
3.1.2
INTRODUCTION
.
61
3.1.3
RESULTS
.
63
3.1.4
DISCUSSION
.
81
3.2
GENETIC
CELL
SURFACE
MODIFICATION
FOR
OPTIMIZED
FOAM
FRACTIONATION
.
89
3.2.1
ABSTRACT
.
89
3.2.2
INTRODUCTION
.
89
3.2.3
RESULTS
.
91
3.2.4
DISCUSSION
.
99
3.3
UNCOUPLING
FOAM
FRACTIONATION
AND
FOAM
ADSORPTION
FOR
ENHANCED
BIOSURFACTANT
SYNTHESIS
AND
RECOVERY
.
107
3.3.1
ABSTRACT
.
107
3.3.2
INTRODUCTION
.
107
3.3.3
RESULTS
.
109
3.3.4
DISCUSSION
.
118
4
GENERAL
DISCUSSION
AND
OUTLOOK
.127
4.1
P.
PUTIDA
K.T2440
AS
IDEAL
RL
AND
HAA
PRODUCTION
HOST
.
127
4.2
BIOREACTOR
PROCESSES
FOR
RL
AND
HAA
PRODUCTION
BY
A
NON-PATHOGENIC
P.
PUTIDA
K.T2440
WITH
FOAM
FRACTIONATION
AS
PREFERRED
TECHNIQUE
FOR
INTEGRATED
PRODUCT
RECOVERY
.
128
4.3
CONVERSION
OF
HAA
INTO
BIOFUEL
BY
ELECTROCHEMICAL
OR
CHEMO-CATALYTIC
DEOXYGENATION
.
130
4.4
CONCLUSION
AND
OUTLOOK
.
131
5
APPENDIX
.
137
5.1
SEPARATION
OF
CIO-CIO
CONGENER
OF
RL
AND
HAA
VIA PREPARATIVE
HPLC
.
137
6
REFERENCES
.
143
CURRICULUM
VITAE
.
159
II |
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| any_adam_object_boolean | 1 |
| author | Blesken, Christian Carl 1984- |
| author_GND | (DE-588)1246995123 |
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| author_role | aut |
| author_sort | Blesken, Christian Carl 1984- |
| author_variant | c c b cc ccb |
| building | Verbundindex |
| bvnumber | BV047634450 |
| collection | ebook |
| ctrlnum | (OCoLC)1287913418 (DE-599)DNB1245634089 |
| doi_str_mv | 10.18154/RWTH-2021-11458 |
| edition | 1. Auflage |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047634450 |
| illustrated | Illustrated |
| index_date | 2024-07-03T18:46:24Z |
| indexdate | 2024-07-10T09:17:47Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783985550074 3985550077 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033018760 |
| oclc_num | 1287913418 |
| open_access_boolean | 1 |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | XV, 162 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 278 g |
| psigel | ebook |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| publisher | Apprimus Verlag |
| record_format | marc |
| series | Applied Microbiology |
| series2 | Applied Microbiology |
| spelling | Blesken, Christian Carl 1984- Verfasser (DE-588)1246995123 aut Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives Christian Carl Blesken 1. Auflage Aachen Apprimus Verlag 2021 XV, 162 Seiten Illustrationen, Diagramme 21 cm x 14.8 cm, 278 g txt rdacontent n rdamedia nc rdacarrier Applied Microbiology Volume 26 Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2021 Schaum (DE-588)4116372-2 gnd rswk-swf Biotensid (DE-588)4297514-1 gnd rswk-swf Herstellung (DE-588)4159653-5 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Prozessführung Technik (DE-588)4197295-8 gnd rswk-swf Ausgangsmaterial (DE-588)4143518-7 gnd rswk-swf Pseudomonas (DE-588)4210233-9 gnd rswk-swf Rhamnolipide (DE-588)4282721-8 gnd rswk-swf Bioreaktor (DE-588)4006780-4 gnd rswk-swf Trennverfahren (DE-588)4078395-9 gnd rswk-swf Rhamnosederivate (DE-588)4543022-6 gnd rswk-swf Naturwissenschaften integrierte Produktabtrennung (DE-588)4113937-9 Hochschulschrift gnd-content Biotechnologie (DE-588)4069491-4 s Bioreaktor (DE-588)4006780-4 s Schaum (DE-588)4116372-2 s Rhamnolipide (DE-588)4282721-8 s Rhamnosederivate (DE-588)4543022-6 s Trennverfahren (DE-588)4078395-9 s DE-604 Biotensid (DE-588)4297514-1 s Herstellung (DE-588)4159653-5 s Pseudomonas (DE-588)4210233-9 s Prozessführung Technik (DE-588)4197295-8 s Ausgangsmaterial (DE-588)4143518-7 s Apprimus Verlag (DE-588)1068101474 pbl Erscheint auch als Online-Ausgabe, PDF https://doi.org/10.18154/RWTH-2021-11458 Applied Microbiology Volume 26 (DE-604)BV042644516 26 https://doi.org/10.18154/RWTH-2021-11458 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033018760&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p vlb 20211112 DE-101 https://d-nb.info/provenance/plan#vlb |
| spellingShingle | Blesken, Christian Carl 1984- Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives Applied Microbiology Schaum (DE-588)4116372-2 gnd Biotensid (DE-588)4297514-1 gnd Herstellung (DE-588)4159653-5 gnd Biotechnologie (DE-588)4069491-4 gnd Prozessführung Technik (DE-588)4197295-8 gnd Ausgangsmaterial (DE-588)4143518-7 gnd Pseudomonas (DE-588)4210233-9 gnd Rhamnolipide (DE-588)4282721-8 gnd Bioreaktor (DE-588)4006780-4 gnd Trennverfahren (DE-588)4078395-9 gnd Rhamnosederivate (DE-588)4543022-6 gnd |
| subject_GND | (DE-588)4116372-2 (DE-588)4297514-1 (DE-588)4159653-5 (DE-588)4069491-4 (DE-588)4197295-8 (DE-588)4143518-7 (DE-588)4210233-9 (DE-588)4282721-8 (DE-588)4006780-4 (DE-588)4078395-9 (DE-588)4543022-6 (DE-588)4113937-9 |
| title | Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives |
| title_auth | Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives |
| title_exact_search | Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives |
| title_exact_search_txtP | Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives |
| title_full | Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives Christian Carl Blesken |
| title_fullStr | Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives Christian Carl Blesken |
| title_full_unstemmed | Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives Christian Carl Blesken |
| title_short | Bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives |
| title_sort | bioreactor and process configurations for the production and recovery of foam intense rhamnolipids and derivatives |
| topic | Schaum (DE-588)4116372-2 gnd Biotensid (DE-588)4297514-1 gnd Herstellung (DE-588)4159653-5 gnd Biotechnologie (DE-588)4069491-4 gnd Prozessführung Technik (DE-588)4197295-8 gnd Ausgangsmaterial (DE-588)4143518-7 gnd Pseudomonas (DE-588)4210233-9 gnd Rhamnolipide (DE-588)4282721-8 gnd Bioreaktor (DE-588)4006780-4 gnd Trennverfahren (DE-588)4078395-9 gnd Rhamnosederivate (DE-588)4543022-6 gnd |
| topic_facet | Schaum Biotensid Herstellung Biotechnologie Prozessführung Technik Ausgangsmaterial Pseudomonas Rhamnolipide Bioreaktor Trennverfahren Rhamnosederivate Hochschulschrift |
| url | https://doi.org/10.18154/RWTH-2021-11458 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033018760&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV042644516 |
| work_keys_str_mv | AT bleskenchristiancarl bioreactorandprocessconfigurationsfortheproductionandrecoveryoffoamintenserhamnolipidsandderivatives AT apprimusverlag bioreactorandprocessconfigurationsfortheproductionandrecoveryoffoamintenserhamnolipidsandderivatives |