Gene expression analysis: methods and protocols
This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics technique...
Gespeichert in:
| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1783 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics techniques, and targeted sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gene Expression Analysis: Methods and Protocols aims provide useful information to researchers worldwide |
| Beschreibung: | Overview of Gene Expression Analysis - Transcriptomics -- RNA-Seq and Expression Arrays: Selection Guidelines for Genome-wide Expression Profiling -- A Guide for Designing and Analysing RNA-Seq Data -- SureSelectXT RNA Direct: A Technique for Expression Analysis Through Sequencing of Target-enriched FFPE total RNA -- Simultaneous, Multiplexed Detection of RNA and Protein on the NanoString nCounter Platform -- Transcript Profiling Using Long-read Sequencing Technologies -- Making and Sequencing Heavily-Multiplexed, High-throughput 16S Ribosomal RNA Gene Amplicon Libraries Using a Flexible, Two-stage PCR PROTOCOL -- microRNA Expression Analysis - Next Generation Sequencing -- Identification of Transcriptional Regulators that Bind to Long Noncoding RNAs by RNA Pull Down and RNA Immunoprecipitation -- Single-cell mRNA-Seq using the Fluidigm C1 System and Integrated Fluidics Circuits -- Current and Future Methods for mRNA Analysis: A Drive Towards Single Molecule Sequencing -- Expression Profiling of Differentially Regulated Genes in Fanconi Anemia -- A Review of Transcriptome Analysis in Pulmonary Vascular Diseases -- Differential Gene Expression Analysis of Plants -- High Throughput Sequencing Based Approaches for Gene Expression Analysis -- Network Analysis of Gene Expression -- Analysis of ChIP-Seq and RNA-Seq data with BioWardrobe -- Bayesian Network to Infer Drug-induced Apoptosis Circuits from Connectivity Map Data. |
| Beschreibung: | 1 Online-Ressource (XI, 387 Seiten) Illustrationen |
| ISBN: | 9781493978342 |
| DOI: | 10.1007/978-1-4939-7834-2 |
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| discipline | Biologie Medizin |
| discipline_str_mv | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-4939-7834-2 |
| edition | 1st ed. 2018 |
| format | Electronic eBook |
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| spelling | Gene expression analysis methods and protocols edited by Nalini Raghavachari, Natàlia Garcia-Reyero 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 387 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1783 Overview of Gene Expression Analysis - Transcriptomics -- RNA-Seq and Expression Arrays: Selection Guidelines for Genome-wide Expression Profiling -- A Guide for Designing and Analysing RNA-Seq Data -- SureSelectXT RNA Direct: A Technique for Expression Analysis Through Sequencing of Target-enriched FFPE total RNA -- Simultaneous, Multiplexed Detection of RNA and Protein on the NanoString nCounter Platform -- Transcript Profiling Using Long-read Sequencing Technologies -- Making and Sequencing Heavily-Multiplexed, High-throughput 16S Ribosomal RNA Gene Amplicon Libraries Using a Flexible, Two-stage PCR PROTOCOL -- microRNA Expression Analysis - Next Generation Sequencing -- Identification of Transcriptional Regulators that Bind to Long Noncoding RNAs by RNA Pull Down and RNA Immunoprecipitation -- Single-cell mRNA-Seq using the Fluidigm C1 System and Integrated Fluidics Circuits -- Current and Future Methods for mRNA Analysis: A Drive Towards Single Molecule Sequencing -- Expression Profiling of Differentially Regulated Genes in Fanconi Anemia -- A Review of Transcriptome Analysis in Pulmonary Vascular Diseases -- Differential Gene Expression Analysis of Plants -- High Throughput Sequencing Based Approaches for Gene Expression Analysis -- Network Analysis of Gene Expression -- Analysis of ChIP-Seq and RNA-Seq data with BioWardrobe -- Bayesian Network to Infer Drug-induced Apoptosis Circuits from Connectivity Map Data. This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics techniques, and targeted sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gene Expression Analysis: Methods and Protocols aims provide useful information to researchers worldwide Human genetics Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Sequenzanalyse Chemie (DE-588)4132277-0 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Genexpression (DE-588)4020136-3 s Sequenzanalyse Chemie (DE-588)4132277-0 s Molekularbiologische Methode (DE-588)7650063-9 s DE-604 Raghavachari, Nalini edt Garcia-Reyero, Natàlia edt Erscheint auch als Druck-Ausgabe 9781493978335 Erscheint auch als Druck-Ausgabe 9781493978359 Erscheint auch als Druck-Ausgabe 9781493992997 https://doi.org/10.1007/978-1-4939-7834-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Gene expression analysis methods and protocols Human genetics Molekularbiologische Methode (DE-588)7650063-9 gnd Sequenzanalyse Chemie (DE-588)4132277-0 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)7650063-9 (DE-588)4132277-0 (DE-588)4020136-3 (DE-588)4143413-4 |
| title | Gene expression analysis methods and protocols |
| title_auth | Gene expression analysis methods and protocols |
| title_exact_search | Gene expression analysis methods and protocols |
| title_exact_search_txtP | Gene expression analysis methods and protocols |
| title_full | Gene expression analysis methods and protocols edited by Nalini Raghavachari, Natàlia Garcia-Reyero |
| title_fullStr | Gene expression analysis methods and protocols edited by Nalini Raghavachari, Natàlia Garcia-Reyero |
| title_full_unstemmed | Gene expression analysis methods and protocols edited by Nalini Raghavachari, Natàlia Garcia-Reyero |
| title_short | Gene expression analysis |
| title_sort | gene expression analysis methods and protocols |
| title_sub | methods and protocols |
| topic | Human genetics Molekularbiologische Methode (DE-588)7650063-9 gnd Sequenzanalyse Chemie (DE-588)4132277-0 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Human genetics Molekularbiologische Methode Sequenzanalyse Chemie Genexpression Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-7834-2 |
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