Schwann cells: methods and protocols
This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and sta...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1739 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of peripheral nerve development, cancer, axon degeneration/regeneration, and myelination. Last but not least, part IV outlines protocols for Schwann cell production, collection, labeling and transplantation in the injured peripheral nerve and spinal cord of experimental animals and human subjects. Authoritative and practical, Schwann Cells: Methods and Protocols aims to aid both experienced and new investigators to make progress in their research endeavors involving Schwann cells |
| Beschreibung: | Isolation of Schwann Cell Precursors from Rodents -- Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Isolation and Expansion of Schwann Cells from Transgenic Mouse Models -- Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers -- Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells -- Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations -- Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies -- Chemical Conversion of Human Fibroblasts into functional Schwann Cells -- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats -- - Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells -- Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay -- Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis -- Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures -- The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes -- In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia -- A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions -- - Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development -- Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs -- 3D Cancer Migration Assay with Schwann Cells -- Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis -- Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration -- The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin -- Analysis of Myelinating Schwann Cells in Human Skin Biopsies -- Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae -- Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin -- Live Imaging of Schwann Cell Development in Zebra Fish -- - Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord -- Schwann Cell Transplantation Methods Using Biomaterials -- Viral Transduction of Schwann Cells for Peripheral Nerve Repair -- Intraspinal Delivery of Schwann Cells for Spinal Cord Injury. |
| Beschreibung: | 1 Online-Ressource (XIII, 488 Seiten) Illustrationen |
| ISBN: | 9781493976492 |
| DOI: | 10.1007/978-1-4939-7649-2 |
Internformat
MARC
| LEADER | 00000nmm a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV047626922 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 211206s2018 |||| o||u| ||||||eng d | ||
| 020 | |a 9781493976492 |c Online |9 978-1-4939-7649-2 | ||
| 024 | 7 | |a 10.1007/978-1-4939-7649-2 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-4939-7649-2 | ||
| 035 | |a (OCoLC)1289760858 | ||
| 035 | |a (DE-599)BVBBV047626922 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 571.6 | |
| 084 | |a WW 4290 |0 (DE-625)152099:13423 |2 rvk | ||
| 245 | 1 | 0 | |a Schwann cells |b methods and protocols |c edited by Paula V. Monje, Haesun A. Kim |
| 250 | |a 1st ed. 2018 | ||
| 264 | 1 | |a New York, NY |b Springer New York |c 2018 | |
| 300 | |a 1 Online-Ressource (XIII, 488 Seiten) |b Illustrationen | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology | |
| 490 | 0 | |a 1739 | |
| 500 | |a Isolation of Schwann Cell Precursors from Rodents -- Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Isolation and Expansion of Schwann Cells from Transgenic Mouse Models -- Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers -- Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells -- Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations -- Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies -- Chemical Conversion of Human Fibroblasts into functional Schwann Cells -- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats -- | ||
| 500 | |a - Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells -- Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay -- Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis -- Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures -- The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes -- In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia -- A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions -- | ||
| 500 | |a - Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development -- Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs -- 3D Cancer Migration Assay with Schwann Cells -- Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis -- Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration -- The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin -- Analysis of Myelinating Schwann Cells in Human Skin Biopsies -- Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae -- Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin -- Live Imaging of Schwann Cell Development in Zebra Fish -- | ||
| 500 | |a - Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord -- Schwann Cell Transplantation Methods Using Biomaterials -- Viral Transduction of Schwann Cells for Peripheral Nerve Repair -- Intraspinal Delivery of Schwann Cells for Spinal Cord Injury. | ||
| 520 | |a This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of peripheral nerve development, cancer, axon degeneration/regeneration, and myelination. Last but not least, part IV outlines protocols for Schwann cell production, collection, labeling and transplantation in the injured peripheral nerve and spinal cord of experimental animals and human subjects. Authoritative and practical, Schwann Cells: Methods and Protocols aims to aid both experienced and new investigators to make progress in their research endeavors involving Schwann cells | ||
| 650 | 4 | |a Cell biology | |
| 650 | 0 | 7 | |a Schwann-Zelle |0 (DE-588)4231189-5 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Schwann-Zelle |0 (DE-588)4231189-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Monje, Paula Virginia |0 (DE-588)1123485151 |4 edt | |
| 700 | 1 | |a Kim, Haesun A. |0 (DE-588)1162468378 |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781493976485 |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781493976508 |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781493992546 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-7649-2 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-033011408 | ||
| 966 | e | |u https://doi.org/10.1007/978-1-4939-7649-2 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-7649-2 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804183069643505664 |
|---|---|
| adam_txt | |
| any_adam_object | |
| any_adam_object_boolean | |
| author2 | Monje, Paula Virginia Kim, Haesun A. |
| author2_role | edt edt |
| author2_variant | p v m pv pvm h a k ha hak |
| author_GND | (DE-588)1123485151 (DE-588)1162468378 |
| author_facet | Monje, Paula Virginia Kim, Haesun A. |
| building | Verbundindex |
| bvnumber | BV047626922 |
| classification_rvk | WW 4290 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-4939-7649-2 (OCoLC)1289760858 (DE-599)BVBBV047626922 |
| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| doi_str_mv | 10.1007/978-1-4939-7649-2 |
| edition | 1st ed. 2018 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>06078nmm a2200541zc 4500</leader><controlfield tag="001">BV047626922</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">211206s2018 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493976492</subfield><subfield code="c">Online</subfield><subfield code="9">978-1-4939-7649-2</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-4939-7649-2</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-4939-7649-2</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1289760858</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV047626922</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WW 4290</subfield><subfield code="0">(DE-625)152099:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Schwann cells</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">edited by Paula V. Monje, Haesun A. Kim</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1st ed. 2018</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer New York</subfield><subfield code="c">2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XIII, 488 Seiten)</subfield><subfield code="b">Illustrationen</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">1739</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Isolation of Schwann Cell Precursors from Rodents -- Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Isolation and Expansion of Schwann Cells from Transgenic Mouse Models -- Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers -- Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells -- Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations -- Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies -- Chemical Conversion of Human Fibroblasts into functional Schwann Cells -- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats -- </subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a"> - Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells -- Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay -- Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis -- Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures -- The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes -- In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia -- A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions -- </subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a"> - Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development -- Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs -- 3D Cancer Migration Assay with Schwann Cells -- Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis -- Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration -- The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin -- Analysis of Myelinating Schwann Cells in Human Skin Biopsies -- Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae -- Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin -- Live Imaging of Schwann Cell Development in Zebra Fish -- </subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a"> - Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord -- Schwann Cell Transplantation Methods Using Biomaterials -- Viral Transduction of Schwann Cells for Peripheral Nerve Repair -- Intraspinal Delivery of Schwann Cells for Spinal Cord Injury.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of peripheral nerve development, cancer, axon degeneration/regeneration, and myelination. Last but not least, part IV outlines protocols for Schwann cell production, collection, labeling and transplantation in the injured peripheral nerve and spinal cord of experimental animals and human subjects. Authoritative and practical, Schwann Cells: Methods and Protocols aims to aid both experienced and new investigators to make progress in their research endeavors involving Schwann cells</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell biology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Schwann-Zelle</subfield><subfield code="0">(DE-588)4231189-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Schwann-Zelle</subfield><subfield code="0">(DE-588)4231189-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Monje, Paula Virginia</subfield><subfield code="0">(DE-588)1123485151</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kim, Haesun A.</subfield><subfield code="0">(DE-588)1162468378</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781493976485</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781493976508</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781493992546</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-4939-7649-2</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-033011408</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-7649-2</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-7649-2</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV047626922 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:44:32Z |
| indexdate | 2024-07-10T09:17:35Z |
| institution | BVB |
| isbn | 9781493976492 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033011408 |
| oclc_num | 1289760858 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XIII, 488 Seiten) Illustrationen |
| psigel | ZDB-2-PRO |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| publisher | Springer New York |
| record_format | marc |
| series2 | Methods in Molecular Biology 1739 |
| spelling | Schwann cells methods and protocols edited by Paula V. Monje, Haesun A. Kim 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XIII, 488 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1739 Isolation of Schwann Cell Precursors from Rodents -- Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Isolation and Expansion of Schwann Cells from Transgenic Mouse Models -- Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers -- Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells -- Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations -- Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies -- Chemical Conversion of Human Fibroblasts into functional Schwann Cells -- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats -- - Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells -- Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay -- Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis -- Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures -- The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes -- In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia -- A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions -- - Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development -- Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs -- 3D Cancer Migration Assay with Schwann Cells -- Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis -- Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration -- The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin -- Analysis of Myelinating Schwann Cells in Human Skin Biopsies -- Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae -- Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin -- Live Imaging of Schwann Cell Development in Zebra Fish -- - Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord -- Schwann Cell Transplantation Methods Using Biomaterials -- Viral Transduction of Schwann Cells for Peripheral Nerve Repair -- Intraspinal Delivery of Schwann Cells for Spinal Cord Injury. This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of peripheral nerve development, cancer, axon degeneration/regeneration, and myelination. Last but not least, part IV outlines protocols for Schwann cell production, collection, labeling and transplantation in the injured peripheral nerve and spinal cord of experimental animals and human subjects. Authoritative and practical, Schwann Cells: Methods and Protocols aims to aid both experienced and new investigators to make progress in their research endeavors involving Schwann cells Cell biology Schwann-Zelle (DE-588)4231189-5 gnd rswk-swf Schwann-Zelle (DE-588)4231189-5 s DE-604 Monje, Paula Virginia (DE-588)1123485151 edt Kim, Haesun A. (DE-588)1162468378 edt Erscheint auch als Druck-Ausgabe 9781493976485 Erscheint auch als Druck-Ausgabe 9781493976508 Erscheint auch als Druck-Ausgabe 9781493992546 https://doi.org/10.1007/978-1-4939-7649-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Schwann cells methods and protocols Cell biology Schwann-Zelle (DE-588)4231189-5 gnd |
| subject_GND | (DE-588)4231189-5 |
| title | Schwann cells methods and protocols |
| title_auth | Schwann cells methods and protocols |
| title_exact_search | Schwann cells methods and protocols |
| title_exact_search_txtP | Schwann cells methods and protocols |
| title_full | Schwann cells methods and protocols edited by Paula V. Monje, Haesun A. Kim |
| title_fullStr | Schwann cells methods and protocols edited by Paula V. Monje, Haesun A. Kim |
| title_full_unstemmed | Schwann cells methods and protocols edited by Paula V. Monje, Haesun A. Kim |
| title_short | Schwann cells |
| title_sort | schwann cells methods and protocols |
| title_sub | methods and protocols |
| topic | Cell biology Schwann-Zelle (DE-588)4231189-5 gnd |
| topic_facet | Cell biology Schwann-Zelle |
| url | https://doi.org/10.1007/978-1-4939-7649-2 |
| work_keys_str_mv | AT monjepaulavirginia schwanncellsmethodsandprotocols AT kimhaesuna schwanncellsmethodsandprotocols |