Cellular heterogeneity: methods and protocols
This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity ana...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1745 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries |
| Beschreibung: | Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level -- Integrating Analysis of Cellular Heterogeneity in High Content Dose-Response Studies -- Image-Based Tracking of Heterogeneous Single Cell Phenotypes -- High-Dimensional Single Cell Analysis with Mass Cytometry -- Spectral and Imaging Flow Cytometry in Phytoplankton Research -- X-Ray Fluorescence Detected Flow Cytometry -- Multiparametric Analysis of Myeloid Populations by Flow Cytometry -- Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry -- Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry -- Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System during Neuroinflammation and Neurodegeneration -- Analysis of Microtubule Dynamics Heterogeneity in Cell Culture -- Heterogeneity of Focal Adhesions and Focal Contacts in Motile Fibroblasts -- Laser Tweezers Raman Micro-Spectroscopy of Single Cells and Biological Particles -- Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the NADH/NAD+ Ratio Using a Genetically-Encoded Sensor.- Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites.- Assessing Carbon Source-Dependent Phenotypic Variability in Pseudomonas putida -- The Retinal Pigment Epithelial Cell Line (ARPE-19) Displays Mosaic Structural Chromosomal Aberrations -- FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA-Sequencing -- Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy |
| Beschreibung: | 1 Online-Ressource (XVI, 363 Seiten) Illustrationen |
| ISBN: | 9781493976805 |
| DOI: | 10.1007/978-1-4939-7680-5 |
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| spelling | Cellular heterogeneity methods and protocols edited by Natasha S. Barteneva, Ivan A. Vorobjev 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XVI, 363 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1745 Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level -- Integrating Analysis of Cellular Heterogeneity in High Content Dose-Response Studies -- Image-Based Tracking of Heterogeneous Single Cell Phenotypes -- High-Dimensional Single Cell Analysis with Mass Cytometry -- Spectral and Imaging Flow Cytometry in Phytoplankton Research -- X-Ray Fluorescence Detected Flow Cytometry -- Multiparametric Analysis of Myeloid Populations by Flow Cytometry -- Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry -- Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry -- Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System during Neuroinflammation and Neurodegeneration -- Analysis of Microtubule Dynamics Heterogeneity in Cell Culture -- Heterogeneity of Focal Adhesions and Focal Contacts in Motile Fibroblasts -- Laser Tweezers Raman Micro-Spectroscopy of Single Cells and Biological Particles -- Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the NADH/NAD+ Ratio Using a Genetically-Encoded Sensor.- Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites.- Assessing Carbon Source-Dependent Phenotypic Variability in Pseudomonas putida -- The Retinal Pigment Epithelial Cell Line (ARPE-19) Displays Mosaic Structural Chromosomal Aberrations -- FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA-Sequencing -- Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries Cell biology Barteneva, Natasha S. (DE-588)1100361383 edt Vorobjev, Ivan A. (DE-588)1155847091 edt Erscheint auch als Druck-Ausgabe 9781493976799 Erscheint auch als Druck-Ausgabe 9781493976812 Erscheint auch als Druck-Ausgabe 9781493985418 https://doi.org/10.1007/978-1-4939-7680-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Cellular heterogeneity methods and protocols Cell biology |
| title | Cellular heterogeneity methods and protocols |
| title_auth | Cellular heterogeneity methods and protocols |
| title_exact_search | Cellular heterogeneity methods and protocols |
| title_exact_search_txtP | Cellular heterogeneity methods and protocols |
| title_full | Cellular heterogeneity methods and protocols edited by Natasha S. Barteneva, Ivan A. Vorobjev |
| title_fullStr | Cellular heterogeneity methods and protocols edited by Natasha S. Barteneva, Ivan A. Vorobjev |
| title_full_unstemmed | Cellular heterogeneity methods and protocols edited by Natasha S. Barteneva, Ivan A. Vorobjev |
| title_short | Cellular heterogeneity |
| title_sort | cellular heterogeneity methods and protocols |
| title_sub | methods and protocols |
| topic | Cell biology |
| topic_facet | Cell biology |
| url | https://doi.org/10.1007/978-1-4939-7680-5 |
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