X-chromosome inactivation: methods and protocols
This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of mol...
Saved in:
| Other Authors: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY
Springer New York
2018
|
| Edition: | 1st ed. 2018 |
| Series: | Methods in Molecular Biology
1861 |
| Subjects: | |
| Online Access: | UBR01 TUM01 Volltext |
| Summary: | This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture |
| Item Description: | Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos -- Somatic Cell Nuclear Transfer in Mice - Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status -- Live Imaging of Xist RNA -- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics -- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes -- Visualization of Hidden Epitopes at the Inactive X Chromosome -- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation -- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos -- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos -- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos -- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation -- X-chromosome Inactivation and Escape from X inactivation in Mouse -- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles |
| Physical Description: | 1 Online-Ressource (XI, 251 Seiten) Illustrationen |
| ISBN: | 9781493987665 |
| DOI: | 10.1007/978-1-4939-8766-5 |
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| dewey-full | 611.01816 599.935 |
| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 611 - Human anatomy, cytology, histology 599 - Mammalia |
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| discipline | Biologie Medizin |
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| doi_str_mv | 10.1007/978-1-4939-8766-5 |
| edition | 1st ed. 2018 |
| format | Electronic eBook |
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| isbn | 9781493987665 |
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| spelling | X-chromosome inactivation methods and protocols edited by Takashi Sado 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 251 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1861 Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos -- Somatic Cell Nuclear Transfer in Mice - Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status -- Live Imaging of Xist RNA -- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics -- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes -- Visualization of Hidden Epitopes at the Inactive X Chromosome -- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation -- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos -- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos -- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos -- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation -- X-chromosome Inactivation and Escape from X inactivation in Mouse -- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture Human genetics X-Chromosom (DE-588)4190368-7 gnd rswk-swf Inaktivierung (DE-588)4284643-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content X-Chromosom (DE-588)4190368-7 s Inaktivierung (DE-588)4284643-2 s DE-604 Sado, Takashi (DE-588)1180417844 edt Erscheint auch als Druck-Ausgabe 978-1-4939-8765-8 Erscheint auch als Druck-Ausgabe 978-1-4939-8767-2 Erscheint auch als Druck-Ausgabe 978-1-4939-9386-4 https://doi.org/10.1007/978-1-4939-8766-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | X-chromosome inactivation methods and protocols Human genetics X-Chromosom (DE-588)4190368-7 gnd Inaktivierung (DE-588)4284643-2 gnd |
| subject_GND | (DE-588)4190368-7 (DE-588)4284643-2 (DE-588)4143413-4 |
| title | X-chromosome inactivation methods and protocols |
| title_auth | X-chromosome inactivation methods and protocols |
| title_exact_search | X-chromosome inactivation methods and protocols |
| title_exact_search_txtP | X-chromosome inactivation methods and protocols |
| title_full | X-chromosome inactivation methods and protocols edited by Takashi Sado |
| title_fullStr | X-chromosome inactivation methods and protocols edited by Takashi Sado |
| title_full_unstemmed | X-chromosome inactivation methods and protocols edited by Takashi Sado |
| title_short | X-chromosome inactivation |
| title_sort | x chromosome inactivation methods and protocols |
| title_sub | methods and protocols |
| topic | Human genetics X-Chromosom (DE-588)4190368-7 gnd Inaktivierung (DE-588)4284643-2 gnd |
| topic_facet | Human genetics X-Chromosom Inaktivierung Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-8766-5 |
| work_keys_str_mv | AT sadotakashi xchromosomeinactivationmethodsandprotocols |