X-chromosome inactivation: methods and protocols
This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of mol...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1861 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture |
| Beschreibung: | Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos -- Somatic Cell Nuclear Transfer in Mice - Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status -- Live Imaging of Xist RNA -- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics -- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes -- Visualization of Hidden Epitopes at the Inactive X Chromosome -- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation -- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos -- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos -- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos -- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation -- X-chromosome Inactivation and Escape from X inactivation in Mouse -- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles |
| Beschreibung: | 1 Online-Ressource (XI, 251 Seiten) Illustrationen |
| ISBN: | 9781493987665 |
| DOI: | 10.1007/978-1-4939-8766-5 |
Internformat
MARC
| LEADER | 00000nmm a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV047625220 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 211206s2018 |||| o||u| ||||||eng d | ||
| 020 | |a 9781493987665 |c Online |9 978-1-4939-8766-5 | ||
| 024 | 7 | |a 10.1007/978-1-4939-8766-5 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-4939-8766-5 | ||
| 035 | |a (OCoLC)1289781338 | ||
| 035 | |a (DE-599)BVBBV047625220 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 611.01816 | |
| 082 | 0 | |a 599.935 | |
| 084 | |a WE 4500 |0 (DE-625)148293: |2 rvk | ||
| 084 | |a WG 1940 |0 (DE-625)148511: |2 rvk | ||
| 245 | 1 | 0 | |a X-chromosome inactivation |b methods and protocols |c edited by Takashi Sado |
| 250 | |a 1st ed. 2018 | ||
| 264 | 1 | |a New York, NY |b Springer New York |c 2018 | |
| 300 | |a 1 Online-Ressource (XI, 251 Seiten) |b Illustrationen | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology | |
| 490 | 0 | |a 1861 | |
| 500 | |a Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos -- Somatic Cell Nuclear Transfer in Mice - Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status -- Live Imaging of Xist RNA -- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics -- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes -- Visualization of Hidden Epitopes at the Inactive X Chromosome -- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation -- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos -- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos -- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos -- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation -- X-chromosome Inactivation and Escape from X inactivation in Mouse -- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles | ||
| 520 | |a This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture | ||
| 650 | 4 | |a Human genetics | |
| 650 | 0 | 7 | |a X-Chromosom |0 (DE-588)4190368-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Inaktivierung |0 (DE-588)4284643-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a X-Chromosom |0 (DE-588)4190368-7 |D s |
| 689 | 0 | 1 | |a Inaktivierung |0 (DE-588)4284643-2 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Sado, Takashi |0 (DE-588)1180417844 |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-8765-8 |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-8767-2 |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-9386-4 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-8766-5 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-033009752 | ||
| 966 | e | |u https://doi.org/10.1007/978-1-4939-8766-5 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-8766-5 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804183066677084160 |
|---|---|
| adam_txt | |
| any_adam_object | |
| any_adam_object_boolean | |
| author2 | Sado, Takashi |
| author2_role | edt |
| author2_variant | t s ts |
| author_GND | (DE-588)1180417844 |
| author_facet | Sado, Takashi |
| building | Verbundindex |
| bvnumber | BV047625220 |
| classification_rvk | WE 4500 WG 1940 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-4939-8766-5 (OCoLC)1289781338 (DE-599)BVBBV047625220 |
| dewey-full | 611.01816 599.935 |
| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 611 - Human anatomy, cytology, histology 599 - Mammalia |
| dewey-raw | 611.01816 599.935 |
| dewey-search | 611.01816 599.935 |
| dewey-sort | 3611.01816 |
| dewey-tens | 610 - Medicine and health 590 - Animals |
| discipline | Biologie Medizin |
| discipline_str_mv | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-4939-8766-5 |
| edition | 1st ed. 2018 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>04186nmm a2200553zc 4500</leader><controlfield tag="001">BV047625220</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">211206s2018 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493987665</subfield><subfield code="c">Online</subfield><subfield code="9">978-1-4939-8766-5</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-4939-8766-5</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-4939-8766-5</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1289781338</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV047625220</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">611.01816</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">599.935</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WE 4500</subfield><subfield code="0">(DE-625)148293:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 1940</subfield><subfield code="0">(DE-625)148511:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">X-chromosome inactivation</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">edited by Takashi Sado</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1st ed. 2018</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer New York</subfield><subfield code="c">2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XI, 251 Seiten)</subfield><subfield code="b">Illustrationen</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">1861</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos -- Somatic Cell Nuclear Transfer in Mice - Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status -- Live Imaging of Xist RNA -- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics -- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes -- Visualization of Hidden Epitopes at the Inactive X Chromosome -- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation -- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos -- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos -- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos -- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation -- X-chromosome Inactivation and Escape from X inactivation in Mouse -- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human genetics</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">X-Chromosom</subfield><subfield code="0">(DE-588)4190368-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Inaktivierung</subfield><subfield code="0">(DE-588)4284643-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">X-Chromosom</subfield><subfield code="0">(DE-588)4190368-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Inaktivierung</subfield><subfield code="0">(DE-588)4284643-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sado, Takashi</subfield><subfield code="0">(DE-588)1180417844</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-8765-8</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-8767-2</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-9386-4</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-4939-8766-5</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-033009752</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-8766-5</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-8766-5</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV047625220 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:44:12Z |
| indexdate | 2024-07-10T09:17:32Z |
| institution | BVB |
| isbn | 9781493987665 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033009752 |
| oclc_num | 1289781338 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XI, 251 Seiten) Illustrationen |
| psigel | ZDB-2-PRO |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| publisher | Springer New York |
| record_format | marc |
| series2 | Methods in Molecular Biology 1861 |
| spelling | X-chromosome inactivation methods and protocols edited by Takashi Sado 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 251 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1861 Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos -- Somatic Cell Nuclear Transfer in Mice - Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status -- Live Imaging of Xist RNA -- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics -- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes -- Visualization of Hidden Epitopes at the Inactive X Chromosome -- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation -- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos -- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos -- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos -- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation -- X-chromosome Inactivation and Escape from X inactivation in Mouse -- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture Human genetics X-Chromosom (DE-588)4190368-7 gnd rswk-swf Inaktivierung (DE-588)4284643-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content X-Chromosom (DE-588)4190368-7 s Inaktivierung (DE-588)4284643-2 s DE-604 Sado, Takashi (DE-588)1180417844 edt Erscheint auch als Druck-Ausgabe 978-1-4939-8765-8 Erscheint auch als Druck-Ausgabe 978-1-4939-8767-2 Erscheint auch als Druck-Ausgabe 978-1-4939-9386-4 https://doi.org/10.1007/978-1-4939-8766-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | X-chromosome inactivation methods and protocols Human genetics X-Chromosom (DE-588)4190368-7 gnd Inaktivierung (DE-588)4284643-2 gnd |
| subject_GND | (DE-588)4190368-7 (DE-588)4284643-2 (DE-588)4143413-4 |
| title | X-chromosome inactivation methods and protocols |
| title_auth | X-chromosome inactivation methods and protocols |
| title_exact_search | X-chromosome inactivation methods and protocols |
| title_exact_search_txtP | X-chromosome inactivation methods and protocols |
| title_full | X-chromosome inactivation methods and protocols edited by Takashi Sado |
| title_fullStr | X-chromosome inactivation methods and protocols edited by Takashi Sado |
| title_full_unstemmed | X-chromosome inactivation methods and protocols edited by Takashi Sado |
| title_short | X-chromosome inactivation |
| title_sort | x chromosome inactivation methods and protocols |
| title_sub | methods and protocols |
| topic | Human genetics X-Chromosom (DE-588)4190368-7 gnd Inaktivierung (DE-588)4284643-2 gnd |
| topic_facet | Human genetics X-Chromosom Inaktivierung Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-8766-5 |
| work_keys_str_mv | AT sadotakashi xchromosomeinactivationmethodsandprotocols |