Mitophagy: methods and protocols
This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1759 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy |
| Beschreibung: | Short Overview of PINK1/Parkin-mediated Mitophagy -- Induction of PINK1/Parkin-Mediated Mitophagy -- Immunocytochemical Monitoring of PINK1/Parkin-mediated Mitophagy in Cultured Cells -- The Use Of Correlative Light-electron Microscopy (CLEM) To Study PINK1/Parkin-mediated Mitophagy -- Observation Of Parkin-mediated Mitophagy in Pancreatic Beta-cells -- Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila -- Assessment of Mitophagy in iPS Cell-derived Neurons -- Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting -- Mitopho8∆60 Assay as a Tool to Quantitatively Measure Mitophagy Activity -- Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants -- Flow Cytometer Monitoring of Bnip3 and Bnip3L/Nix-dependent Mitophagy -- Exploring Micrornas on Nix-Dependent Mitophagy -- Monitoring of Atg5-Independent Mitophagy -- Monitoring of Paternal Mitochondrial Degradation In Caenorhabditis Elegans -- Detection of Hypoxia-induced and Iron Depletion-induced Mitophagy in Mammalian Cells -- Monitoring Mitophagy during Ageing in Caenorhabditis Elegans -- Iron-Starvation Induced Mitophagy in Yeast |
| Beschreibung: | 1 Online-Ressource (XI, 180 Seiten) Illustrationen |
| ISBN: | 9781493977505 |
| DOI: | 10.1007/978-1-4939-7750-5 |
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| 520 | |a This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy | ||
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| edition | 1st ed. 2018 |
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| isbn | 9781493977505 |
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| spelling | Mitophagy methods and protocols edited by Nobutaka Hattori, Shinji Saiki 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 180 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1759 Short Overview of PINK1/Parkin-mediated Mitophagy -- Induction of PINK1/Parkin-Mediated Mitophagy -- Immunocytochemical Monitoring of PINK1/Parkin-mediated Mitophagy in Cultured Cells -- The Use Of Correlative Light-electron Microscopy (CLEM) To Study PINK1/Parkin-mediated Mitophagy -- Observation Of Parkin-mediated Mitophagy in Pancreatic Beta-cells -- Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila -- Assessment of Mitophagy in iPS Cell-derived Neurons -- Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting -- Mitopho8∆60 Assay as a Tool to Quantitatively Measure Mitophagy Activity -- Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants -- Flow Cytometer Monitoring of Bnip3 and Bnip3L/Nix-dependent Mitophagy -- Exploring Micrornas on Nix-Dependent Mitophagy -- Monitoring of Atg5-Independent Mitophagy -- Monitoring of Paternal Mitochondrial Degradation In Caenorhabditis Elegans -- Detection of Hypoxia-induced and Iron Depletion-induced Mitophagy in Mammalian Cells -- Monitoring Mitophagy during Ageing in Caenorhabditis Elegans -- Iron-Starvation Induced Mitophagy in Yeast This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy Cell biology Hattori, Nobutaka ca. 20./21. Jh. (DE-588)1246905094 edt Saiki, Shinji ca. 20./21. Jh. (DE-588)1246905574 edt Erscheint auch als Druck-Ausgabe 978-1-4939-7749-9 Erscheint auch als Druck-Ausgabe 978-1-4939-7751-2 Erscheint auch als Druck-Ausgabe 978-1-4939-9274-4 https://doi.org/10.1007/978-1-4939-7750-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Mitophagy methods and protocols Cell biology |
| title | Mitophagy methods and protocols |
| title_auth | Mitophagy methods and protocols |
| title_exact_search | Mitophagy methods and protocols |
| title_exact_search_txtP | Mitophagy methods and protocols |
| title_full | Mitophagy methods and protocols edited by Nobutaka Hattori, Shinji Saiki |
| title_fullStr | Mitophagy methods and protocols edited by Nobutaka Hattori, Shinji Saiki |
| title_full_unstemmed | Mitophagy methods and protocols edited by Nobutaka Hattori, Shinji Saiki |
| title_short | Mitophagy |
| title_sort | mitophagy methods and protocols |
| title_sub | methods and protocols |
| topic | Cell biology |
| topic_facet | Cell biology |
| url | https://doi.org/10.1007/978-1-4939-7750-5 |
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