This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system. The chapters in this book cover topics such as biochemical binding techniques; receptor autoradiography...

Ausführliche Beschreibung

Bibliographische Detailangaben

Weitere Verfasser:	Fuxe, Kjell 1938- (HerausgeberIn), Borroto-Escuela, Dasiel Oscar 1979- (HerausgeberIn)
Format:	Elektronisch E-Book
Sprache:	English
Veröffentlicht:	New York, NY Springer New York 2018
Ausgabe:	1st ed. 2018
Schriftenreihe:	Neuromethods 140
Schlagworte:	Neurosciences
Online-Zugang:	UBR01 TUM01 Volltext
Zusammenfassung:	This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system. The chapters in this book cover topics such as biochemical binding techniques; receptor autoradiography; superfused synaptosome techniques; RTK-GPCR interaction; fluorescence and bioluminiscence energy transfer methods, Co-IP cytometry-based FRET; and novel bioinformatic approaches to understand membrane heteroreceptor complexes and the global panorama of their receptor-receptor interactions. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Receptor-Receptor Interactions in the Central Nervous System is a valuable resource for any scientist or researcher interested in this field of study
Beschreibung:	Analysis and Quantification of GPCR Allosteric Receptor-Receptor Interactions using Radioligand Binding Assays: The A2AR-D2R Heteroreceptor Complex Example -- Analysis and Quantification of GPCR Heteroreceptor Complexes and their Allosteric Receptor-Receptor Interactions using Radioligand Binding Autoradiography -- On the Study of D4R-Mor Receptor-Receptor Interactions in the Rat Caudate Putamen: Relevance on Morphine Addiction -- Use of Superfused Synaptosomes to Understand the Role of Receptor-Receptor Interactions as Integrative Mechanisms in Nerve Terminals from Selected Brain Region -- Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholine Receptor (mAChRs) in Primary Neuronal Hippocampal Cultures through use of Biochemical and Morphological Approaches -- Electrophysiological Approach to GPCR-RTK Interaction Study in Hippocampus of Adult Rats -- - In Vivo Microdialysis Technique Applications to Understand the Contribution of Receptor-Receptor Interactions to the Central Nervous System Signaling -- Behavioral Methods to Study the Impact of Receptor-Recepetor Interactions in Fear and Anxiety -- Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxiety and Depression-Like Behavior -- Double Fluorescent Knock-In-Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Distribution -- Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays -- Methods to Identify the Signature of Trimers Formed by Three G Protein-Coupled Receptors, or by Two G Protein-Coupled and One Ionotropic Receptor with Special Emphasis in the Functional Role in the Central Nervous System -- Biomolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells -- - Detection and Quantitative Analysis of Dynamic GPCRs Interactions using Flow Cytometry-Based FRET -- Assessing GPCR Dimerization in Living Cells: Comparison of the NanoBiT Assay with Related Bioluminescence- and Fluorescence-Based Approaches -- Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization -- Conformational Profiling of the 5-HT2A Receptor Using F1AsH-BRET -- Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor-Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions -- Detection, Analysis, and Quantification of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay -- Unraveling the Functions of Endogenous Receptor Oligomers in the Brain using Interfering-Peptide: The Example of D1R/NMDAR Heteromers -- Super-Resolution Imaging as a Method to Study GPCR Dimers and Higher-Order Oligomers
Beschreibung:	1 Online-Ressource (XIX, 346 Seiten) Illustrationen
ISBN:	9781493985760
DOI:	10.1007/978-1-4939-8576-0