Plant chemical genomics: methods and protocols
This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-ce...
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| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1795 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches |
| Beschreibung: | Multi-Parametric Screening in Arabidopsis thaliana Seedlings -- Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress -- Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen -- A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana -- High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression -- Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics -- Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells -- Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes -- High-Throughput In Vitro Screening for Inhibitors of Cereal α-glucosidase -- Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana -- Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress -- Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening -- Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman -- Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule -- Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH -- Chemical Genomics Translatability from Unicellular to Multicellular Models -- Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii -- FnCpf1-Mediated Targeted Mutagenesis in Plants |
| Beschreibung: | 1 Online-Ressource (XI, 242 Seiten) Illustrationen |
| ISBN: | 9781493978748 |
| DOI: | 10.1007/978-1-4939-7874-8 |
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| dewey-full | 580 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 580 - Plants |
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| dewey-search | 580 |
| dewey-sort | 3580 |
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| discipline | Biologie |
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| edition | 1st ed. 2018 |
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| spelling | Plant chemical genomics methods and protocols edited by Friedrich Fauser, Martin Jonikas 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 242 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1795 Multi-Parametric Screening in Arabidopsis thaliana Seedlings -- Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress -- Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen -- A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana -- High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression -- Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics -- Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells -- Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes -- High-Throughput In Vitro Screening for Inhibitors of Cereal α-glucosidase -- Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana -- Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress -- Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening -- Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman -- Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule -- Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH -- Chemical Genomics Translatability from Unicellular to Multicellular Models -- Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii -- FnCpf1-Mediated Targeted Mutagenesis in Plants This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches Plant science Botany Fauser, Friedrich 1985- (DE-588)106023663X edt Jonikas, Martin ca. 20./21. Jh. (DE-588)1162205334 edt Erscheint auch als Druck-Ausgabe 978-1-4939-7873-1 Erscheint auch als Druck-Ausgabe 978-1-4939-7875-5 Erscheint auch als Druck-Ausgabe 978-1-4939-9311-6 https://doi.org/10.1007/978-1-4939-7874-8 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Plant chemical genomics methods and protocols Plant science Botany |
| title | Plant chemical genomics methods and protocols |
| title_auth | Plant chemical genomics methods and protocols |
| title_exact_search | Plant chemical genomics methods and protocols |
| title_exact_search_txtP | Plant chemical genomics methods and protocols |
| title_full | Plant chemical genomics methods and protocols edited by Friedrich Fauser, Martin Jonikas |
| title_fullStr | Plant chemical genomics methods and protocols edited by Friedrich Fauser, Martin Jonikas |
| title_full_unstemmed | Plant chemical genomics methods and protocols edited by Friedrich Fauser, Martin Jonikas |
| title_short | Plant chemical genomics |
| title_sort | plant chemical genomics methods and protocols |
| title_sub | methods and protocols |
| topic | Plant science Botany |
| topic_facet | Plant science Botany |
| url | https://doi.org/10.1007/978-1-4939-7874-8 |
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