CpG islands: methods and protocols
This detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer. B...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1766 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer. Beginning with a historical perspective and important properties of CpG islands, the book continues with sections on computational and wet lab methods related to the study of DNA methylation, and in-depth protocols for the analysis of CpG island functional features including epigenetic profiling and chromatin interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, CpG Islands: Methods and Protocols aims to provide readers with the information and methodologies necessary to continue to decipher how a genome's structure and organization contribute to regulate biological processes |
| Beschreibung: | CpG Islands: A Historical Perspective -- Biochemical Identification of Non-Methylated DNA by BioCAP-Seq -- Prediction of CpG Islands as an Intrinsic Clustering Property Found in Many Eukaryotic DNA Sequences and Its Relation to DNA Methylation -- CpG Islands in Cancer: Heads, Tails, and Sides.- Infinium DNA Methylation Microarrays on Formalin-Fixed Paraffin-Embedded Samples.- The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation -- The Pancancer DNA Methylation Trackhub: A Window to The Cancer Genome Atlas Epigenomics Data -- Methylation-Sensitive Amplification Length Polymorphism (MS-AFLP) Microarrays for Epigenetic Analysis of Human Genomes -- Genome-Wide Profiling of DNA Methyltransferases in Mammalian Cells -- Experimental Design and Bioinformatic Analysis of DNA Methylation Data -- Assay for Transposase Accessible Chromatin (ATAC-Seq) to Chart the Open Chromatin Landscape of Human Pancreatic Islets -- Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-Seq) -- Genome-Wide Mapping of Protein-DNA Interactions on Nascent Chromatin -- Analysis of Chromatin Interactions Mediated by Specific Architectural Proteins in Drosophila Cells -- High-Throughput Single-Cell RNA Sequencing and Data Analysis -- Functional Insulator Scanning of CpG Islands to Identify Regulatory Regions of Promoters Using CRISPR -- An Application-Directed, Versatile DNA FISH Platform for Research and Diagnostics |
| Beschreibung: | 1 Online-Ressource (XI, 340 Seiten) Illustrationen |
| ISBN: | 9781493977680 |
| DOI: | 10.1007/978-1-4939-7768-0 |
Internformat
MARC
| LEADER | 00000nmm a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV047614362 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 211129s2018 |||| o||u| ||||||eng d | ||
| 020 | |a 9781493977680 |c Online |9 978-1-4939-7768-0 | ||
| 024 | 7 | |a 10.1007/978-1-4939-7768-0 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-4939-7768-0 | ||
| 035 | |a (OCoLC)1286879431 | ||
| 035 | |a (DE-599)BVBBV047614362 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 611.01816 | |
| 082 | 0 | |a 599.935 | |
| 084 | |a WC 4460 |0 (DE-625)148102: |2 rvk | ||
| 084 | |a WC 7700 |0 (DE-625)148144: |2 rvk | ||
| 245 | 1 | 0 | |a CpG islands |b methods and protocols |c edited by Tanya Vavouri, Miguel A. Peinado |
| 250 | |a 1st ed. 2018 | ||
| 264 | 1 | |a New York, NY |b Springer New York |c 2018 | |
| 300 | |a 1 Online-Ressource (XI, 340 Seiten) |b Illustrationen | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology | |
| 490 | 0 | |a 1766 | |
| 500 | |a CpG Islands: A Historical Perspective -- Biochemical Identification of Non-Methylated DNA by BioCAP-Seq -- Prediction of CpG Islands as an Intrinsic Clustering Property Found in Many Eukaryotic DNA Sequences and Its Relation to DNA Methylation -- CpG Islands in Cancer: Heads, Tails, and Sides.- Infinium DNA Methylation Microarrays on Formalin-Fixed Paraffin-Embedded Samples.- The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation -- The Pancancer DNA Methylation Trackhub: A Window to The Cancer Genome Atlas Epigenomics Data -- Methylation-Sensitive Amplification Length Polymorphism (MS-AFLP) Microarrays for Epigenetic Analysis of Human Genomes -- Genome-Wide Profiling of DNA Methyltransferases in Mammalian Cells -- Experimental Design and Bioinformatic Analysis of DNA Methylation Data -- Assay for Transposase Accessible Chromatin (ATAC-Seq) to Chart the Open Chromatin Landscape of Human Pancreatic Islets -- Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-Seq) -- Genome-Wide Mapping of Protein-DNA Interactions on Nascent Chromatin -- Analysis of Chromatin Interactions Mediated by Specific Architectural Proteins in Drosophila Cells -- High-Throughput Single-Cell RNA Sequencing and Data Analysis -- Functional Insulator Scanning of CpG Islands to Identify Regulatory Regions of Promoters Using CRISPR -- An Application-Directed, Versatile DNA FISH Platform for Research and Diagnostics | ||
| 520 | |a This detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer. Beginning with a historical perspective and important properties of CpG islands, the book continues with sections on computational and wet lab methods related to the study of DNA methylation, and in-depth protocols for the analysis of CpG island functional features including epigenetic profiling and chromatin interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, CpG Islands: Methods and Protocols aims to provide readers with the information and methodologies necessary to continue to decipher how a genome's structure and organization contribute to regulate biological processes | ||
| 650 | 4 | |a Human genetics | |
| 700 | 1 | |a Vavouri, Tanya |d ca. 20./21. Jh. |0 (DE-588)1246567091 |4 edt | |
| 700 | 1 | |a Peinado, Miguel A. |d ca. 20./21. Jh. |0 (DE-588)1246567210 |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-7767-3 |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-7769-7 |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-9279-9 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-7768-0 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032999118 | ||
| 966 | e | |u https://doi.org/10.1007/978-1-4939-7768-0 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-7768-0 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804182983979040768 |
|---|---|
| adam_txt | |
| any_adam_object | |
| any_adam_object_boolean | |
| author2 | Vavouri, Tanya ca. 20./21. Jh Peinado, Miguel A. ca. 20./21. Jh |
| author2_role | edt edt |
| author2_variant | t v tv m a p ma map |
| author_GND | (DE-588)1246567091 (DE-588)1246567210 |
| author_facet | Vavouri, Tanya ca. 20./21. Jh Peinado, Miguel A. ca. 20./21. Jh |
| building | Verbundindex |
| bvnumber | BV047614362 |
| classification_rvk | WC 4460 WC 7700 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-4939-7768-0 (OCoLC)1286879431 (DE-599)BVBBV047614362 |
| dewey-full | 611.01816 599.935 |
| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 611 - Human anatomy, cytology, histology 599 - Mammalia |
| dewey-raw | 611.01816 599.935 |
| dewey-search | 611.01816 599.935 |
| dewey-sort | 3611.01816 |
| dewey-tens | 610 - Medicine and health 590 - Animals |
| discipline | Biologie Medizin |
| discipline_str_mv | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-4939-7768-0 |
| edition | 1st ed. 2018 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>04509nmm a2200493zc 4500</leader><controlfield tag="001">BV047614362</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">211129s2018 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493977680</subfield><subfield code="c">Online</subfield><subfield code="9">978-1-4939-7768-0</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-4939-7768-0</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-4939-7768-0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1286879431</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV047614362</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">611.01816</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">599.935</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4460</subfield><subfield code="0">(DE-625)148102:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 7700</subfield><subfield code="0">(DE-625)148144:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">CpG islands</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">edited by Tanya Vavouri, Miguel A. Peinado</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1st ed. 2018</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer New York</subfield><subfield code="c">2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XI, 340 Seiten)</subfield><subfield code="b">Illustrationen</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">1766</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">CpG Islands: A Historical Perspective -- Biochemical Identification of Non-Methylated DNA by BioCAP-Seq -- Prediction of CpG Islands as an Intrinsic Clustering Property Found in Many Eukaryotic DNA Sequences and Its Relation to DNA Methylation -- CpG Islands in Cancer: Heads, Tails, and Sides.- Infinium DNA Methylation Microarrays on Formalin-Fixed Paraffin-Embedded Samples.- The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation -- The Pancancer DNA Methylation Trackhub: A Window to The Cancer Genome Atlas Epigenomics Data -- Methylation-Sensitive Amplification Length Polymorphism (MS-AFLP) Microarrays for Epigenetic Analysis of Human Genomes -- Genome-Wide Profiling of DNA Methyltransferases in Mammalian Cells -- Experimental Design and Bioinformatic Analysis of DNA Methylation Data -- Assay for Transposase Accessible Chromatin (ATAC-Seq) to Chart the Open Chromatin Landscape of Human Pancreatic Islets -- Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-Seq) -- Genome-Wide Mapping of Protein-DNA Interactions on Nascent Chromatin -- Analysis of Chromatin Interactions Mediated by Specific Architectural Proteins in Drosophila Cells -- High-Throughput Single-Cell RNA Sequencing and Data Analysis -- Functional Insulator Scanning of CpG Islands to Identify Regulatory Regions of Promoters Using CRISPR -- An Application-Directed, Versatile DNA FISH Platform for Research and Diagnostics</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer. Beginning with a historical perspective and important properties of CpG islands, the book continues with sections on computational and wet lab methods related to the study of DNA methylation, and in-depth protocols for the analysis of CpG island functional features including epigenetic profiling and chromatin interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, CpG Islands: Methods and Protocols aims to provide readers with the information and methodologies necessary to continue to decipher how a genome's structure and organization contribute to regulate biological processes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human genetics</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Vavouri, Tanya</subfield><subfield code="d">ca. 20./21. Jh.</subfield><subfield code="0">(DE-588)1246567091</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Peinado, Miguel A.</subfield><subfield code="d">ca. 20./21. Jh.</subfield><subfield code="0">(DE-588)1246567210</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-7767-3</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-7769-7</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-9279-9</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-4939-7768-0</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-032999118</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-7768-0</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-7768-0</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV047614362 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:40:52Z |
| indexdate | 2024-07-10T09:16:13Z |
| institution | BVB |
| isbn | 9781493977680 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032999118 |
| oclc_num | 1286879431 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XI, 340 Seiten) Illustrationen |
| psigel | ZDB-2-PRO |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| publisher | Springer New York |
| record_format | marc |
| series2 | Methods in Molecular Biology 1766 |
| spelling | CpG islands methods and protocols edited by Tanya Vavouri, Miguel A. Peinado 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XI, 340 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1766 CpG Islands: A Historical Perspective -- Biochemical Identification of Non-Methylated DNA by BioCAP-Seq -- Prediction of CpG Islands as an Intrinsic Clustering Property Found in Many Eukaryotic DNA Sequences and Its Relation to DNA Methylation -- CpG Islands in Cancer: Heads, Tails, and Sides.- Infinium DNA Methylation Microarrays on Formalin-Fixed Paraffin-Embedded Samples.- The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation -- The Pancancer DNA Methylation Trackhub: A Window to The Cancer Genome Atlas Epigenomics Data -- Methylation-Sensitive Amplification Length Polymorphism (MS-AFLP) Microarrays for Epigenetic Analysis of Human Genomes -- Genome-Wide Profiling of DNA Methyltransferases in Mammalian Cells -- Experimental Design and Bioinformatic Analysis of DNA Methylation Data -- Assay for Transposase Accessible Chromatin (ATAC-Seq) to Chart the Open Chromatin Landscape of Human Pancreatic Islets -- Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-Seq) -- Genome-Wide Mapping of Protein-DNA Interactions on Nascent Chromatin -- Analysis of Chromatin Interactions Mediated by Specific Architectural Proteins in Drosophila Cells -- High-Throughput Single-Cell RNA Sequencing and Data Analysis -- Functional Insulator Scanning of CpG Islands to Identify Regulatory Regions of Promoters Using CRISPR -- An Application-Directed, Versatile DNA FISH Platform for Research and Diagnostics This detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer. Beginning with a historical perspective and important properties of CpG islands, the book continues with sections on computational and wet lab methods related to the study of DNA methylation, and in-depth protocols for the analysis of CpG island functional features including epigenetic profiling and chromatin interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, CpG Islands: Methods and Protocols aims to provide readers with the information and methodologies necessary to continue to decipher how a genome's structure and organization contribute to regulate biological processes Human genetics Vavouri, Tanya ca. 20./21. Jh. (DE-588)1246567091 edt Peinado, Miguel A. ca. 20./21. Jh. (DE-588)1246567210 edt Erscheint auch als Druck-Ausgabe 978-1-4939-7767-3 Erscheint auch als Druck-Ausgabe 978-1-4939-7769-7 Erscheint auch als Druck-Ausgabe 978-1-4939-9279-9 https://doi.org/10.1007/978-1-4939-7768-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | CpG islands methods and protocols Human genetics |
| title | CpG islands methods and protocols |
| title_auth | CpG islands methods and protocols |
| title_exact_search | CpG islands methods and protocols |
| title_exact_search_txtP | CpG islands methods and protocols |
| title_full | CpG islands methods and protocols edited by Tanya Vavouri, Miguel A. Peinado |
| title_fullStr | CpG islands methods and protocols edited by Tanya Vavouri, Miguel A. Peinado |
| title_full_unstemmed | CpG islands methods and protocols edited by Tanya Vavouri, Miguel A. Peinado |
| title_short | CpG islands |
| title_sort | cpg islands methods and protocols |
| title_sub | methods and protocols |
| topic | Human genetics |
| topic_facet | Human genetics |
| url | https://doi.org/10.1007/978-1-4939-7768-0 |
| work_keys_str_mv | AT vavouritanya cpgislandsmethodsandprotocols AT peinadomiguela cpgislandsmethodsandprotocols |