Adipose-derived stem cells: methods and protocols
This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused...
Gespeichert in:
| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 2nd ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1773 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application |
| Beschreibung: | Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose Tissue -- A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Timeframe -- Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem Cells -- The Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells -- Methylcellulose Based Thermally Reversible Hydrogels -- Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery -- Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells -- High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered Substitutes -- Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery -- Myogenic Differentiation of ASCs using Biochemical and Biophysical Induction -- Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis -- Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue -- Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates -- Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method -- Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation -- Soft Tissue Reconstruction -- Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain -- Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells -- Bone Regeneration with a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma -- Isolation and Differentiation of Murine Primary Brown/Beige Preadipocytes |
| Beschreibung: | 1 Online-Ressource (XII, 284 Seiten) Illustrationen |
| ISBN: | 9781493977994 |
| DOI: | 10.1007/978-1-4939-7799-4 |
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| 520 | |a This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application | ||
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| index_date | 2024-07-03T18:40:42Z |
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| language | English |
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| spelling | Adipose-derived stem cells methods and protocols edited by Bruce A. Bunnell, Jeffrey M. Gimble 2nd ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XII, 284 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1773 Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose Tissue -- A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Timeframe -- Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem Cells -- The Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells -- Methylcellulose Based Thermally Reversible Hydrogels -- Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery -- Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells -- High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered Substitutes -- Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery -- Myogenic Differentiation of ASCs using Biochemical and Biophysical Induction -- Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis -- Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue -- Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates -- Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method -- Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation -- Soft Tissue Reconstruction -- Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain -- Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells -- Bone Regeneration with a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma -- Isolation and Differentiation of Murine Primary Brown/Beige Preadipocytes This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application Cell biology Bunnell, Bruce A. ca. 20./21. Jh. (DE-588)1161689214 edt Gimble, Jeffrey M. ca. 20./21. Jh. (DE-588)1161689273 edt Erscheint auch als Druck-Ausgabe 978-1-4939-7797-0 Erscheint auch als Druck-Ausgabe 978-1-4939-7798-7 Erscheint auch als Druck-Ausgabe 978-1-4939-9288-1 https://doi.org/10.1007/978-1-4939-7799-4 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Adipose-derived stem cells methods and protocols Cell biology |
| title | Adipose-derived stem cells methods and protocols |
| title_auth | Adipose-derived stem cells methods and protocols |
| title_exact_search | Adipose-derived stem cells methods and protocols |
| title_exact_search_txtP | Adipose-derived stem cells methods and protocols |
| title_full | Adipose-derived stem cells methods and protocols edited by Bruce A. Bunnell, Jeffrey M. Gimble |
| title_fullStr | Adipose-derived stem cells methods and protocols edited by Bruce A. Bunnell, Jeffrey M. Gimble |
| title_full_unstemmed | Adipose-derived stem cells methods and protocols edited by Bruce A. Bunnell, Jeffrey M. Gimble |
| title_short | Adipose-derived stem cells |
| title_sort | adipose derived stem cells methods and protocols |
| title_sub | methods and protocols |
| topic | Cell biology |
| topic_facet | Cell biology |
| url | https://doi.org/10.1007/978-1-4939-7799-4 |
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