Circular RNAs: methods and protocols
This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1724 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field. |
| Beschreibung: | Detection and Reconstruction of Circular RNAs from Transcriptomic Data -- Deep Computational Circular RNA Analytics from RNA-seq Data -- Genome-wide circRNA Profiling from RNA-seq Data -- Analysis of Circular RNAs using the Web Tool CircInteractome -- Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR -- Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization -- Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as -- A Highly-efficient Strategy for Overexpressing circRNAs -- Constructing GFP-based Reporter to study Back-splicing and Translation of Circular RNA -- Northern Blot Analysis of Circular RNAs -- Non-radioactive Northern Blot of circRNAs -- Characterization of Circular RNA Concatemers -- Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery -- Synthesis and Engineering of Circular RNAs -- Preparation of Circular RNA in vitro -- Discovering circRNA-microRNA Interactions from CLIP-Seq Data -- Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays. |
| Beschreibung: | 1 Online-Ressource (XIII, 220 Seiten) Illustrationen |
| ISBN: | 9781493975624 |
| DOI: | 10.1007/978-1-4939-7562-4 |
Internformat
MARC
| LEADER | 00000nmm a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV047613000 | ||
| 003 | DE-604 | ||
| 005 | 20211129 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 211126s2018 |||| o||u| ||||||eng d | ||
| 020 | |a 9781493975624 |c Online |9 978-1-4939-7562-4 | ||
| 024 | 7 | |a 10.1007/978-1-4939-7562-4 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-4939-7562-4 | ||
| 035 | |a (OCoLC)1286877701 | ||
| 035 | |a (DE-599)BVBBV047613000 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 599.935 | |
| 082 | 0 | |a 611.01816 | |
| 084 | |a WD 5355 |0 (DE-625)148201: |2 rvk | ||
| 084 | |a WC 4460 |0 (DE-625)148102: |2 rvk | ||
| 245 | 1 | 0 | |a Circular RNAs |b methods and protocols |c edited by Christoph Dieterich, Argyris Papantonis |
| 250 | |a 1st ed. 2018 | ||
| 264 | 1 | |a New York, NY |b Springer New York |c 2018 | |
| 300 | |a 1 Online-Ressource (XIII, 220 Seiten) |b Illustrationen | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology | |
| 490 | 0 | |a 1724 | |
| 500 | |a Detection and Reconstruction of Circular RNAs from Transcriptomic Data -- Deep Computational Circular RNA Analytics from RNA-seq Data -- Genome-wide circRNA Profiling from RNA-seq Data -- Analysis of Circular RNAs using the Web Tool CircInteractome -- Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR -- Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization -- Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as -- A Highly-efficient Strategy for Overexpressing circRNAs -- Constructing GFP-based Reporter to study Back-splicing and Translation of Circular RNA -- Northern Blot Analysis of Circular RNAs -- Non-radioactive Northern Blot of circRNAs -- Characterization of Circular RNA Concatemers -- Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery -- Synthesis and Engineering of Circular RNAs -- Preparation of Circular RNA in vitro -- Discovering circRNA-microRNA Interactions from CLIP-Seq Data -- Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays. | ||
| 520 | |a This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field. | ||
| 650 | 4 | |a Human genetics | |
| 650 | 0 | 7 | |a Zirkuläre RNA |0 (DE-588)1143866711 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Untersuchungsmethode |0 (DE-588)4324907-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Zirkuläre RNA |0 (DE-588)1143866711 |D s |
| 689 | 0 | 1 | |a Untersuchungsmethode |0 (DE-588)4324907-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Dieterich, Christoph |d 1975- |0 (DE-588)130054844 |4 edt | |
| 700 | 1 | |a Papantōnēs, Akēs |d 1978- |0 (DE-588)1073142272 |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-7561-7 |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-7563-1 |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-1-4939-8521-0 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-7562-4 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032997790 | ||
| 966 | e | |u https://doi.org/10.1007/978-1-4939-7562-4 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-7562-4 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804182981694193664 |
|---|---|
| adam_txt | |
| any_adam_object | |
| any_adam_object_boolean | |
| author2 | Dieterich, Christoph 1975- Papantōnēs, Akēs 1978- |
| author2_role | edt edt |
| author2_variant | c d cd a p ap |
| author_GND | (DE-588)130054844 (DE-588)1073142272 |
| author_facet | Dieterich, Christoph 1975- Papantōnēs, Akēs 1978- |
| building | Verbundindex |
| bvnumber | BV047613000 |
| classification_rvk | WD 5355 WC 4460 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-4939-7562-4 (OCoLC)1286877701 (DE-599)BVBBV047613000 |
| dewey-full | 599.935 611.01816 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 599 - Mammalia 611 - Human anatomy, cytology, histology |
| dewey-raw | 599.935 611.01816 |
| dewey-search | 599.935 611.01816 |
| dewey-sort | 3599.935 |
| dewey-tens | 590 - Animals 610 - Medicine and health |
| discipline | Biologie Medizin |
| discipline_str_mv | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-4939-7562-4 |
| edition | 1st ed. 2018 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03965nmm a2200565zc 4500</leader><controlfield tag="001">BV047613000</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20211129 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">211126s2018 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493975624</subfield><subfield code="c">Online</subfield><subfield code="9">978-1-4939-7562-4</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-4939-7562-4</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-4939-7562-4</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1286877701</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV047613000</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">599.935</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">611.01816</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5355</subfield><subfield code="0">(DE-625)148201:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4460</subfield><subfield code="0">(DE-625)148102:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Circular RNAs</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">edited by Christoph Dieterich, Argyris Papantonis</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1st ed. 2018</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer New York</subfield><subfield code="c">2018</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XIII, 220 Seiten)</subfield><subfield code="b">Illustrationen</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">1724</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Detection and Reconstruction of Circular RNAs from Transcriptomic Data -- Deep Computational Circular RNA Analytics from RNA-seq Data -- Genome-wide circRNA Profiling from RNA-seq Data -- Analysis of Circular RNAs using the Web Tool CircInteractome -- Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR -- Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization -- Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as -- A Highly-efficient Strategy for Overexpressing circRNAs -- Constructing GFP-based Reporter to study Back-splicing and Translation of Circular RNA -- Northern Blot Analysis of Circular RNAs -- Non-radioactive Northern Blot of circRNAs -- Characterization of Circular RNA Concatemers -- Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery -- Synthesis and Engineering of Circular RNAs -- Preparation of Circular RNA in vitro -- Discovering circRNA-microRNA Interactions from CLIP-Seq Data -- Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays. </subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field. </subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human genetics</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zirkuläre RNA</subfield><subfield code="0">(DE-588)1143866711</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Untersuchungsmethode</subfield><subfield code="0">(DE-588)4324907-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Zirkuläre RNA</subfield><subfield code="0">(DE-588)1143866711</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Untersuchungsmethode</subfield><subfield code="0">(DE-588)4324907-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dieterich, Christoph</subfield><subfield code="d">1975-</subfield><subfield code="0">(DE-588)130054844</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Papantōnēs, Akēs</subfield><subfield code="d">1978-</subfield><subfield code="0">(DE-588)1073142272</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-7561-7</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-7563-1</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-1-4939-8521-0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-4939-7562-4</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-032997790</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-7562-4</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-7562-4</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV047613000 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T18:40:24Z |
| indexdate | 2024-07-10T09:16:11Z |
| institution | BVB |
| isbn | 9781493975624 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032997790 |
| oclc_num | 1286877701 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XIII, 220 Seiten) Illustrationen |
| psigel | ZDB-2-PRO |
| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| publisher | Springer New York |
| record_format | marc |
| series2 | Methods in Molecular Biology 1724 |
| spelling | Circular RNAs methods and protocols edited by Christoph Dieterich, Argyris Papantonis 1st ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XIII, 220 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1724 Detection and Reconstruction of Circular RNAs from Transcriptomic Data -- Deep Computational Circular RNA Analytics from RNA-seq Data -- Genome-wide circRNA Profiling from RNA-seq Data -- Analysis of Circular RNAs using the Web Tool CircInteractome -- Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR -- Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization -- Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as -- A Highly-efficient Strategy for Overexpressing circRNAs -- Constructing GFP-based Reporter to study Back-splicing and Translation of Circular RNA -- Northern Blot Analysis of Circular RNAs -- Non-radioactive Northern Blot of circRNAs -- Characterization of Circular RNA Concatemers -- Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery -- Synthesis and Engineering of Circular RNAs -- Preparation of Circular RNA in vitro -- Discovering circRNA-microRNA Interactions from CLIP-Seq Data -- Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays. This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field. Human genetics Zirkuläre RNA (DE-588)1143866711 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zirkuläre RNA (DE-588)1143866711 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Dieterich, Christoph 1975- (DE-588)130054844 edt Papantōnēs, Akēs 1978- (DE-588)1073142272 edt Erscheint auch als Druck-Ausgabe 978-1-4939-7561-7 Erscheint auch als Druck-Ausgabe 978-1-4939-7563-1 Erscheint auch als Druck-Ausgabe 978-1-4939-8521-0 https://doi.org/10.1007/978-1-4939-7562-4 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Circular RNAs methods and protocols Human genetics Zirkuläre RNA (DE-588)1143866711 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| subject_GND | (DE-588)1143866711 (DE-588)4324907-3 (DE-588)4143413-4 |
| title | Circular RNAs methods and protocols |
| title_auth | Circular RNAs methods and protocols |
| title_exact_search | Circular RNAs methods and protocols |
| title_exact_search_txtP | Circular RNAs methods and protocols |
| title_full | Circular RNAs methods and protocols edited by Christoph Dieterich, Argyris Papantonis |
| title_fullStr | Circular RNAs methods and protocols edited by Christoph Dieterich, Argyris Papantonis |
| title_full_unstemmed | Circular RNAs methods and protocols edited by Christoph Dieterich, Argyris Papantonis |
| title_short | Circular RNAs |
| title_sort | circular rnas methods and protocols |
| title_sub | methods and protocols |
| topic | Human genetics Zirkuläre RNA (DE-588)1143866711 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| topic_facet | Human genetics Zirkuläre RNA Untersuchungsmethode Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-7562-4 |
| work_keys_str_mv | AT dieterichchristoph circularrnasmethodsandprotocols AT papantonesakes circularrnasmethodsandprotocols |