Transgenic Mouse: Methods and Protocols
This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer...
Gespeichert in:
| Format: | Elektronisch E-Book |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2020
|
| Ausgabe: | 1st ed. 2020 |
| Schriftenreihe: | Methods in Molecular Biology
2066 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. |
| Beschreibung: | The History of Transgenesis -- Pronuclear Microinjection of One-Cell Embryos -- Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection -- In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos -- Targeted Mutations in the Mouse via Embryonic Stem Cells -- Blastocyst Microinjection with Embryonic Stem Cells -- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos -- Embryo Transfer Surgery -- Non-Surgical Embryo Transfer Protocol for use with the NSET™ Device -- Nuclear Transfer and Cloning -- Sperm-Mediated Genetic Modifications -- Genotyping Genetically Modified (GM) Mice -- Nomenclature: Naming Your Gene-Modified Mouse -- Breeding Strategies for Genetically Modified Mice -- Strategies for Behaviorally Phenotyping the Transgenic Mouse -- Cryobanking and Recovery of Genetically Modified Mice -- Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques -- Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus |
| Beschreibung: | 1 Online-Ressource (X, 236 Seiten) Illustrationen |
| ISBN: | 9781493998371 |
| DOI: | 10.1007/978-1-4939-9837-1 |
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Datensatz im Suchindex
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| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 599 - Mammalia 611 - Human anatomy, cytology, histology |
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| dewey-sort | 3599.935 |
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| discipline | Biologie Medizin |
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| doi_str_mv | 10.1007/978-1-4939-9837-1 |
| edition | 1st ed. 2020 |
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| index_date | 2024-07-03T18:38:41Z |
| indexdate | 2024-07-10T09:16:02Z |
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| isbn | 9781493998371 |
| language | English |
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| spelling | Transgenic Mouse Methods and Protocols edited by Melissa A. Larson 1st ed. 2020 New York, NY Springer US 2020 1 Online-Ressource (X, 236 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2066 The History of Transgenesis -- Pronuclear Microinjection of One-Cell Embryos -- Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection -- In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos -- Targeted Mutations in the Mouse via Embryonic Stem Cells -- Blastocyst Microinjection with Embryonic Stem Cells -- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos -- Embryo Transfer Surgery -- Non-Surgical Embryo Transfer Protocol for use with the NSET™ Device -- Nuclear Transfer and Cloning -- Sperm-Mediated Genetic Modifications -- Genotyping Genetically Modified (GM) Mice -- Nomenclature: Naming Your Gene-Modified Mouse -- Breeding Strategies for Genetically Modified Mice -- Strategies for Behaviorally Phenotyping the Transgenic Mouse -- Cryobanking and Recovery of Genetically Modified Mice -- Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques -- Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. Human genetics Animal models in research Stem cells Methode (DE-588)4038971-6 gnd rswk-swf Transgener Organismus (DE-588)4314075-0 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Maus (DE-588)4169148-9 s Transgener Organismus (DE-588)4314075-0 s Methode (DE-588)4038971-6 s DE-604 Larson, Melissa A. Sonstige (DE-588)1196114625 oth Erscheint auch als Druck-Ausgabe 9781493998364 Erscheint auch als Druck-Ausgabe 9781493998388 Erscheint auch als Druck-Ausgabe 9781493998395 https://doi.org/10.1007/978-1-4939-9837-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Transgenic Mouse Methods and Protocols Human genetics Animal models in research Stem cells Methode (DE-588)4038971-6 gnd Transgener Organismus (DE-588)4314075-0 gnd Maus (DE-588)4169148-9 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4314075-0 (DE-588)4169148-9 (DE-588)4143413-4 |
| title | Transgenic Mouse Methods and Protocols |
| title_auth | Transgenic Mouse Methods and Protocols |
| title_exact_search | Transgenic Mouse Methods and Protocols |
| title_exact_search_txtP | Transgenic Mouse Methods and Protocols |
| title_full | Transgenic Mouse Methods and Protocols edited by Melissa A. Larson |
| title_fullStr | Transgenic Mouse Methods and Protocols edited by Melissa A. Larson |
| title_full_unstemmed | Transgenic Mouse Methods and Protocols edited by Melissa A. Larson |
| title_short | Transgenic Mouse |
| title_sort | transgenic mouse methods and protocols |
| title_sub | Methods and Protocols |
| topic | Human genetics Animal models in research Stem cells Methode (DE-588)4038971-6 gnd Transgener Organismus (DE-588)4314075-0 gnd Maus (DE-588)4169148-9 gnd |
| topic_facet | Human genetics Animal models in research Stem cells Methode Transgener Organismus Maus Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-9837-1 |
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