Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing:
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Format: | Abschlussarbeit Buch |
Sprache: | English |
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Kaiserslautern
Technische Universität Kaiserslautern
2021
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Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | VI, 114 Seiten Illustrationen, Diagramme |
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245 | 1 | 0 | |a Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing |c vorgelegt von Tatjana Schmitt |
264 | 1 | |a Kaiserslautern |b Technische Universität Kaiserslautern |c 2021 | |
300 | |a VI, 114 Seiten |b Illustrationen, Diagramme | ||
336 | |b txt |2 rdacontent | ||
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502 | |b Dissertation |c Universität Kaiserslautern |d 2021 | ||
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Datensatz im Suchindex
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adam_text | I
INDEX
OF
CONTENTS
I
INDEX
OF
CONTENTS
........................................................................................................................................
I
II
SUMMARY
..................................................................................................................................................
IV
III
INDEX
OF
FIGURES
........................................................................................................................................
V
IV
INDEX
OF
TABLES
........................................................................................................................................
VI
1
INTRODUCTION
..............................................................................................................................................
1
1.1
FUNCTIONAL
ORGANIZATION
OF
THE
AC
....................................................................................................
1
1.1.1
FREQUENCY
TUNING
........................................................................................................................
1
1.1.2
TONOTOPY
IN
AC
SUBFIELDS
...........................................................................................................
2
1.1.3
FUNCTIONAL
ROLE
OF
AC
SUBFIELDS
..................................................................................................
3
1.1.4
NEURONAL
NETWORKS
IN
THE
AC
....................................................................................................
5
1.2
THE
AUDITORY
CORTICOFUGAL
SYSTEM
.....................................................................................................
6
1.2.1
MORPHOLOGICAL
AND
ELECTROPHYSIOLOGICAL DIFFERENCES
BETWEEN CORTICOFUGAL
AND
CORTICOCORTICAL
NEURONS
IN
L5
..............................................................................................................
7
1.2.2
CC
PATHWAYS
...............................................................................................................................
8
1.2.3
CORTICO-CN
PATHWAY
..................................................................................................................
9
1.3
AIMS
OF
THE
STUDY
............................................................................................................................
10
2
MATERIAL
AND
METHODS
............................................................................................................................
11
2.1
ANIMALS
............................................................................................................................................
11
2.2
STEREOTACTIC
INJECTIONS
....................................................................................................................
11
2.2.1
VIRAL
VECTORS
..............................................................................................................................
11
2.2.2
GENERAL
INJECTION
PROCEDURE
...................................................................................................
12
2.2.3
AC
INJECTION
...............................................................................................................................
13
2.2.4
AUDITORY
BRAINSTEM
INJECTION
...................................................................................................
14
2.3
WINDOW
IMPLANTATION
....................................................................................................................
15
2.4
TRANSCARDIAL
PERFUSION
AND
PREPARATION
OF
HISTOLOGICAL
BRAIN
SLICES
............................................
15
2.5
PREPARATION
OF
ACUTE
BRAIN
SLICES
...................................................................................................
16
2.6
MICROSCOPY
......................................................................................................................................
17
2.7
PATCH-CLAMP
SETUP
AND
ELECTROPHYSIOLOGICAL
RECORDINGS
..............................................................
17
2.8
IN
VIVO
IMAGING
...............................................................................................................................
18
2.8.1
TWO-PHOTON
IMAGING
...............................................................................................................
18
2.8.2
WIDEFIELD
IMAGING
....................................................................................................................
18
2.9
ACOUSTIC
STIMULATION
.......................................................................................................................
19
2.10
DATA
ANALYSIS
................................................................................................................................20
2.10.1
WIDEFIELD
ANALYSIS
..................................................................................................................20
2.10.2
TWO-PHOTON
ANALYSIS
.............................................................................................................
22
2.11
DATA
VISUALIZATION
AND
STATISTICS
..................................................................................................
26
3
RESULTS
....................................................................................................................................................
27
3.1
ESTABLISHMENT
OF
VARIOUS
EXPERIMENTAL
METHODS
FOR
CHARACTERIZATION
OF
CORTICOFUGAL
NEURONS
27
3.1.1
RETROGRADE
LABELLING
OF
THE
MGB
CONFIRMS
AC
AS
INJECTION
SITE
............................................
27
3.1.2
OPTOGENETIC
ACTIVATION
OF
CORTICOFUGAL
PROJECTIONS
...............................................................
29
3.1.3
IDENTIFYING
IC
AND
CN-PROJECTING
NEURONS
IN
THE
AC
...........................................................
33
3.1.4
IDEAL
INJECTION
DEPTH
TO
IMAGE
ACTIVITY
IN
AC
15
....................................................................
35
3.1.5
TWO-PHOTON
CA
2
*
IMAGING
IN
AC
L5
.........................................................................................37
3.2
DIFFERENT
RESPONSE
PROPERTIES
OF
CC
AND
NON-CC
NEURONS
UPON
PT
STIMULATION
.........................
39
3.2.1
CC
AND
NON-CC
NEURONS
HAVE
COMMON
TUNING
PROPERTIES
....................................................
41
3.2.2
IN
L5,
TONOTOPY
AXES
ON
SINGLE-CELL
LEVEL
CAN
ONLY
BE
IDENTIFIED
IN
AL
...................................
48
3.2.3
CC
NEURONS
LACK
A
LINEAR
TONOTOPIC
GRADIENT
IN
AL
.................................................................
51
3.2.4
HETEROGENEITY
OF
LOCAL
BF
DISTRIBUTION
IN
L5
...........................................................................
52
3.2.5
INFLUENCE
OF
THE
COMPLEXITY
OF
FREQUENCY
TUNING
ON
THE
TONOTOPIC
ORGANIZATION
...............
54
3.3
COMPLEX
SOUND
PROCESSING
IN
15
CC
AND
NON-CC
NEURONS
............................................................
57
3.3.1
CC(2)
NEURONS
FORM
CLUSTERS
WITH
NON-CC
NEURONS,
BUT
ARE
SPATIALLY
CLOSER
TOGETHER
.......
57
3.3.2
CLUSTER
WITH
HIGHEST
CORRELATION
OF
THE
NEURONS
ACTIVITY
PATTERNS
ARE
FOUND
IN
AL
...........
60
3.3.3
CC(1)
NEURONS
IN
AL
FORM
WEAKER
CLUSTERS
COMPARED
TO
ALL
15
NEURONS
...............................64
4
DISCUSSION
.............................................................................................................................................
70
4.1
TECHNICAL
CONSIDERATIONS
..........................................................................................................
71
II
4.1.1
DIFFERENCES
OF
TWO
LIGHT-ACTIVATABLE
ION
CHANNELS
WITH
DIFFERENT
KINETICS
............................
71
4.1.2
IMAGING
OF
CC
NEURONS
IN
DEEP
LAYERS
OF
THE
AC
....................................................................71
4.2
CC
AND
NON-CC
NEURONS
SHARE
COMMON
TUNING
PROPERTIES
..........................................................
73
4.3
COMPLEXITY
TONOTOPY
IN
L5
.............................................................................................................77
4.3.1
LINEAR
TONOTOPIC
ORGANIZATION
IN
L5
IS
LIMITED
TO
AL
..............................................................77
4.3.2
TONOTOPY
OF
LS
CC
NEURONS
.....................................................................................................79
4.3.3
COMPLEXITY
OF
FRAS
IN
LS
.........................................................................................................
81
4.4
INVOLVEMENT
OF
CC
NEURONS
DURING
COMPLEX
SOUND
PROCESSING
....................................................
83
4.4.1
INFLUENCES
OF
THE
EXPERIMENTAL
APPROACH
.............................................................................
83
4.4.2
CC
AND
NON-CC
NEURONS
INTERMINGLE
DURING
COMPLEX
SOUND
PROCESSING,
BUT
DISPLAY
DIFFERENT
SUBFIELD-DEPENDENT
NETWORK
FEATURES
............................................................................................
84
4.4.3
FURTHER
EVIDENCE
OF
A
LACKING
HIERARCHY
IN
CLUSTERS
CONTAINING
ONLY
CC
NEURONS
.................87
4.5
CONCLUDING
REMARKS
...................................................................................................................89
4.6
OUTLOOK
........................................................................................................................................90
5
REFERENCES
..............................................................................................................................................91
6
APPENDIX
...............................................................................................................................................104
7
INDEX
OF
ABBREVIATIONS
..........................................................................................................................109
8
CURRICULUM
VITAE
...................................................................................................................................
ILL
9
ACKNOWLEDGEMENTS
..............................................................................................................................
113
III
|
adam_txt |
I
INDEX
OF
CONTENTS
I
INDEX
OF
CONTENTS
.
I
II
SUMMARY
.
IV
III
INDEX
OF
FIGURES
.
V
IV
INDEX
OF
TABLES
.
VI
1
INTRODUCTION
.
1
1.1
FUNCTIONAL
ORGANIZATION
OF
THE
AC
.
1
1.1.1
FREQUENCY
TUNING
.
1
1.1.2
TONOTOPY
IN
AC
SUBFIELDS
.
2
1.1.3
FUNCTIONAL
ROLE
OF
AC
SUBFIELDS
.
3
1.1.4
NEURONAL
NETWORKS
IN
THE
AC
.
5
1.2
THE
AUDITORY
CORTICOFUGAL
SYSTEM
.
6
1.2.1
MORPHOLOGICAL
AND
ELECTROPHYSIOLOGICAL DIFFERENCES
BETWEEN CORTICOFUGAL
AND
CORTICOCORTICAL
NEURONS
IN
L5
.
7
1.2.2
CC
PATHWAYS
.
8
1.2.3
CORTICO-CN
PATHWAY
.
9
1.3
AIMS
OF
THE
STUDY
.
10
2
MATERIAL
AND
METHODS
.
11
2.1
ANIMALS
.
11
2.2
STEREOTACTIC
INJECTIONS
.
11
2.2.1
VIRAL
VECTORS
.
11
2.2.2
GENERAL
INJECTION
PROCEDURE
.
12
2.2.3
AC
INJECTION
.
13
2.2.4
AUDITORY
BRAINSTEM
INJECTION
.
14
2.3
WINDOW
IMPLANTATION
.
15
2.4
TRANSCARDIAL
PERFUSION
AND
PREPARATION
OF
HISTOLOGICAL
BRAIN
SLICES
.
15
2.5
PREPARATION
OF
ACUTE
BRAIN
SLICES
.
16
2.6
MICROSCOPY
.
17
2.7
PATCH-CLAMP
SETUP
AND
ELECTROPHYSIOLOGICAL
RECORDINGS
.
17
2.8
IN
VIVO
IMAGING
.
18
2.8.1
TWO-PHOTON
IMAGING
.
18
2.8.2
WIDEFIELD
IMAGING
.
18
2.9
ACOUSTIC
STIMULATION
.
19
2.10
DATA
ANALYSIS
.20
2.10.1
WIDEFIELD
ANALYSIS
.20
2.10.2
TWO-PHOTON
ANALYSIS
.
22
2.11
DATA
VISUALIZATION
AND
STATISTICS
.
26
3
RESULTS
.
27
3.1
ESTABLISHMENT
OF
VARIOUS
EXPERIMENTAL
METHODS
FOR
CHARACTERIZATION
OF
CORTICOFUGAL
NEURONS
27
3.1.1
RETROGRADE
LABELLING
OF
THE
MGB
CONFIRMS
AC
AS
INJECTION
SITE
.
27
3.1.2
OPTOGENETIC
ACTIVATION
OF
CORTICOFUGAL
PROJECTIONS
.
29
3.1.3
IDENTIFYING
IC
AND
CN-PROJECTING
NEURONS
IN
THE
AC
.
33
3.1.4
IDEAL
INJECTION
DEPTH
TO
IMAGE
ACTIVITY
IN
AC
15
.
35
3.1.5
TWO-PHOTON
CA
2
*
IMAGING
IN
AC
L5
.37
3.2
DIFFERENT
RESPONSE
PROPERTIES
OF
CC
AND
NON-CC
NEURONS
UPON
PT
STIMULATION
.
39
3.2.1
CC
AND
NON-CC
NEURONS
HAVE
COMMON
TUNING
PROPERTIES
.
41
3.2.2
IN
L5,
TONOTOPY
AXES
ON
SINGLE-CELL
LEVEL
CAN
ONLY
BE
IDENTIFIED
IN
AL
.
48
3.2.3
CC
NEURONS
LACK
A
LINEAR
TONOTOPIC
GRADIENT
IN
AL
.
51
3.2.4
HETEROGENEITY
OF
LOCAL
BF
DISTRIBUTION
IN
L5
.
52
3.2.5
INFLUENCE
OF
THE
COMPLEXITY
OF
FREQUENCY
TUNING
ON
THE
TONOTOPIC
ORGANIZATION
.
54
3.3
COMPLEX
SOUND
PROCESSING
IN
15
CC
AND
NON-CC
NEURONS
.
57
3.3.1
CC(2)
NEURONS
FORM
CLUSTERS
WITH
NON-CC
NEURONS,
BUT
ARE
SPATIALLY
CLOSER
TOGETHER
.
57
3.3.2
CLUSTER
WITH
HIGHEST
CORRELATION
OF
THE
NEURONS'
ACTIVITY
PATTERNS
ARE
FOUND
IN
AL
.
60
3.3.3
CC(1)
NEURONS
IN
AL
FORM
WEAKER
CLUSTERS
COMPARED
TO
ALL
15
NEURONS
.64
4
DISCUSSION
.
70
4.1
TECHNICAL
CONSIDERATIONS
.
71
II
4.1.1
DIFFERENCES
OF
TWO
LIGHT-ACTIVATABLE
ION
CHANNELS
WITH
DIFFERENT
KINETICS
.
71
4.1.2
IMAGING
OF
CC
NEURONS
IN
DEEP
LAYERS
OF
THE
AC
.71
4.2
CC
AND
NON-CC
NEURONS
SHARE
COMMON
TUNING
PROPERTIES
.
73
4.3
COMPLEXITY
TONOTOPY
IN
L5
.77
4.3.1
LINEAR
TONOTOPIC
ORGANIZATION
IN
L5
IS
LIMITED
TO
AL
.77
4.3.2
TONOTOPY
OF
LS
CC
NEURONS
.79
4.3.3
COMPLEXITY
OF
FRAS
IN
LS
.
81
4.4
INVOLVEMENT
OF
CC
NEURONS
DURING
COMPLEX
SOUND
PROCESSING
.
83
4.4.1
INFLUENCES
OF
THE
EXPERIMENTAL
APPROACH
.
83
4.4.2
CC
AND
NON-CC
NEURONS
INTERMINGLE
DURING
COMPLEX
SOUND
PROCESSING,
BUT
DISPLAY
DIFFERENT
SUBFIELD-DEPENDENT
NETWORK
FEATURES
.
84
4.4.3
FURTHER
EVIDENCE
OF
A
LACKING
HIERARCHY
IN
CLUSTERS
CONTAINING
ONLY
CC
NEURONS
.87
4.5
CONCLUDING
REMARKS
.89
4.6
OUTLOOK
.90
5
REFERENCES
.91
6
APPENDIX
.104
7
INDEX
OF
ABBREVIATIONS
.109
8
CURRICULUM
VITAE
.
ILL
9
ACKNOWLEDGEMENTS
.
113
III |
any_adam_object | 1 |
any_adam_object_boolean | 1 |
author | Schmitt, Tatjana 1993- |
author_GND | (DE-588)1243123729 |
author_facet | Schmitt, Tatjana 1993- |
author_role | aut |
author_sort | Schmitt, Tatjana 1993- |
author_variant | t s ts |
building | Verbundindex |
bvnumber | BV047493626 |
ctrlnum | (OCoLC)1277020789 (DE-599)DNB1242050345 |
format | Thesis Book |
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genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
genre_facet | Hochschulschrift |
id | DE-604.BV047493626 |
illustrated | Illustrated |
index_date | 2024-07-03T18:16:14Z |
indexdate | 2024-07-10T09:13:38Z |
institution | BVB |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032894848 |
oclc_num | 1277020789 |
open_access_boolean | |
owner | DE-29T DE-Aug7 |
owner_facet | DE-29T DE-Aug7 |
physical | VI, 114 Seiten Illustrationen, Diagramme |
publishDate | 2021 |
publishDateSearch | 2021 |
publishDateSort | 2021 |
publisher | Technische Universität Kaiserslautern |
record_format | marc |
spelling | Schmitt, Tatjana 1993- Verfasser (DE-588)1243123729 aut Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing vorgelegt von Tatjana Schmitt Kaiserslautern Technische Universität Kaiserslautern 2021 VI, 114 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Kaiserslautern 2021 Hörrinde (DE-588)4192229-3 gnd rswk-swf Neurobiologie (DE-588)4041871-6 gnd rswk-swf Lautwahrnehmung (DE-588)4125741-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Neurobiologie (DE-588)4041871-6 s Lautwahrnehmung (DE-588)4125741-8 s Hörrinde (DE-588)4192229-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032894848&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Schmitt, Tatjana 1993- Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing Hörrinde (DE-588)4192229-3 gnd Neurobiologie (DE-588)4041871-6 gnd Lautwahrnehmung (DE-588)4125741-8 gnd |
subject_GND | (DE-588)4192229-3 (DE-588)4041871-6 (DE-588)4125741-8 (DE-588)4113937-9 |
title | Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing |
title_auth | Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing |
title_exact_search | Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing |
title_exact_search_txtP | Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing |
title_full | Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing vorgelegt von Tatjana Schmitt |
title_fullStr | Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing vorgelegt von Tatjana Schmitt |
title_full_unstemmed | Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing vorgelegt von Tatjana Schmitt |
title_short | Auditory corticofugal neurons: Tuning, tonotopy and complex sound processing |
title_sort | auditory corticofugal neurons tuning tonotopy and complex sound processing |
topic | Hörrinde (DE-588)4192229-3 gnd Neurobiologie (DE-588)4041871-6 gnd Lautwahrnehmung (DE-588)4125741-8 gnd |
topic_facet | Hörrinde Neurobiologie Lautwahrnehmung Hochschulschrift |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032894848&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
work_keys_str_mv | AT schmitttatjana auditorycorticofugalneuronstuningtonotopyandcomplexsoundprocessing |