The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Aachen
[2020]
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | VIII, 67 Seiten Illustrationen, Diagramme 21 cm |
Staff View
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV047464423 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 210913s2020 gw a||| m||| 00||| eng d | ||
| 015 | |a 21,H10 |2 dnb | ||
| 016 | 7 | |a 1236371984 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1268187891 | ||
| 035 | |a (DE-599)DNB1236371984 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 610 |2 sdnb | ||
| 084 | |8 2\p |a 610 |2 23sdnb | ||
| 084 | |8 1\p |a 616.2 |2 23ksdnb | ||
| 100 | 1 | |a Benninghoven-Frey, Hannah Louise |d 1994- |e Verfasser |0 (DE-588)122612447X |4 aut | |
| 245 | 1 | 0 | |a The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |c vorgelegt von Hannah Louise Benninghoven-Frey |
| 264 | 1 | |a Aachen |c [2020] | |
| 300 | |a VIII, 67 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Rheinisch-Westfälische Technische Hochschule Aachen |d 2020 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1236371984/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032866148&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032866148 | ||
| 883 | 0 | |8 1\p |a aepkn |c 0,72150 |d 20210901 |q DE-101 |u https://d-nb.info/provenance/plan#aepkn | |
| 883 | 2 | |8 2\p |a dnb |d 20210906 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
Record in the Search Index
| _version_ | 1804182773409251328 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
ABSTRACT
.................................................................................................................................
I
ZUSAMMENFASSUNG
..............................................................................................................
II
TABLE
OF
CONTENTS
...............................................................................................................
ILL
INDEX
OF
FIGURES
...................................................................................................................
V
INDEX
OF
TABLES
...................................................................................................................
VI
LIST
OF
ABBREVIATIONS
........................................................................................................
VII
1
INTRODUCTION
................................................................................................................
1
1.1
FUNCTION
AND
STRUCTURE
OF
THE
PRIMARY
CILIUM
............................................................
1
1.2
LIFE
CYCLE
OF
THE
PRIMARY
CILIUM
..................................................................................
4
1.3
PHOSPHOINOSITIDES
IN
EUKARYOTIC
CELLS
.........................................................................
6
1.3.1
PHOSPHOINOSITIDES
IN
THE
PRIMARY
CILIUM
.................................................................
8
1.4
CILIOPATHIES
.................................................................................................................
8
1.4.1
JOUBERT
SYNDROME
...................................................................................................
9
1.5
CHEMICALLY-INDUCIBLE
DIMERIZATION
(CID)
..................................................................
10
2
AIMS
AND
OBJECTIVES
.................................................................................................
12
3
MATERIAL
AND
METHODS
..............................................................................................
13
3.1
MATERIAL
....................................................................................................................
13
3.1.1
BUFFERS,
SOLUTIONS
AND
REAGENTS
...........................................................................
13
3.1.2
MEDIA
....................................................................................................................
14
3.1.3
TRANSFECTION
REAGENT
............................................................................................
15
3.1.4
KITS
.........................................................................................................................
15
3.1.5
ENZYMES
................................................................................................................
15
3.1.6
EQUIPMENT
.............................................................................................................
16
3.1.7
SOFTWARE
................................................................................................................
17
3.1.8
BACTERIA
.................................................................................................................
18
3.1.9
PLASMID
DESCRIPTION
...............................................................................................
19
3.2
METHODS
....................................................................................................................
25
3.2.1
CELL
CULTURE
............................................................................................................
25
3.2.2
FLUORESCENCE
MICROSCOPY
......................................................................................
27
3.2.3
DRUG
APPLICATION
....................................................................................................
28
3.2.4
DATA
ANALYSIS
.........................................................................................................
29
3.2.5
CLONING
...................................................................................................................
30
3.2.6
SEQUENCING
OF
PLASMIDS
.........................................................................................
33
4
RESULTS
........................................................................................................................
34
4.1
CONTRIBUTION
..............................................................................................................
34
4.2
PH(AKT)
IS
RESTRICTED
TO
THE
BASAL
75
PERCENT
OF
THE
PRIMARY
CILIUM
........................
34
4.3
CILIARY
PH(AKT)
CAN
BE
MANIPULATED
BY
CHEMICALLY-INDUCIBLE
DIMERIZATION
...........
36
4.4
THE
PI3K
INHIBITOR
LY294002
REDUCES
THE
RATE
OF
PIPK-INDUCED
CILIARY
FISSION
.......
39
4.5
PI3K
CONTRIBUTES
TO
THE
PROCESS
OF
CILIARY
FISSION
IN
NIH
3T3
CELLS
...........................
42
5
DISCUSSION
..................................................................................................................
45
5.1
DETECTION
OF
CILIARY
PI(3,4,5)P
3
USING
THE
PLECKSTRIN
HOMOLOGY
DOMAIN
OF
AKT
.....
45
5.2
THE
INFLUENCE
OF
CONSTITUTIVE
EXPRESSION
AND
ACUTE
RECRUITMENT
OF
PIPK
ON
CILIARY
PI(3,4,5)P3
LEVELS
AND
STABILITY
...............................................................................................
48
5.3
THE
INFLUENCE
OF
CONSTITUTIVE
EXPRESSION
OF
INP54P
ON
CILIARY
PH(AKT)
LEVELS
AND
CILIARY
LENGTH
..........................................................................................................................
49
5.4
THE
INFLUENCE
OF
PI3K
CLASS
I
RECRUITMENT
ON
CILIARY
PI(3,4,5)P
3
LEVELS
AND
LENGTH..
50
5.5
ENDOGENOUS
PI3K
IS
INVOLVED
IN
THE
PROCESS
OF
CILIARY
FISSION
..................................
51
5.6
LIMITATIONS
AND
REMAINING
QUESTIONS
......................................................................
54
5.7
THERAPEUTIC
APPROACH
FOR
CILIOPATHIES
.....................................................................
56
6
CONCLUSION
.................................................................................................................
57
7
REFERENCES
.................................................................................................................
58
8
ACKNOWLEDGEMENTS
...................................................................................................
63
9
DECLARATIONS
...............................................................................................................
64
10
CURRICULUM
VITAE
...................................................................................................
66
IV
|
| adam_txt |
TABLE
OF
CONTENTS
ABSTRACT
.
I
ZUSAMMENFASSUNG
.
II
TABLE
OF
CONTENTS
.
ILL
INDEX
OF
FIGURES
.
V
INDEX
OF
TABLES
.
VI
LIST
OF
ABBREVIATIONS
.
VII
1
INTRODUCTION
.
1
1.1
FUNCTION
AND
STRUCTURE
OF
THE
PRIMARY
CILIUM
.
1
1.2
LIFE
CYCLE
OF
THE
PRIMARY
CILIUM
.
4
1.3
PHOSPHOINOSITIDES
IN
EUKARYOTIC
CELLS
.
6
1.3.1
PHOSPHOINOSITIDES
IN
THE
PRIMARY
CILIUM
.
8
1.4
CILIOPATHIES
.
8
1.4.1
JOUBERT
SYNDROME
.
9
1.5
CHEMICALLY-INDUCIBLE
DIMERIZATION
(CID)
.
10
2
AIMS
AND
OBJECTIVES
.
12
3
MATERIAL
AND
METHODS
.
13
3.1
MATERIAL
.
13
3.1.1
BUFFERS,
SOLUTIONS
AND
REAGENTS
.
13
3.1.2
MEDIA
.
14
3.1.3
TRANSFECTION
REAGENT
.
15
3.1.4
KITS
.
15
3.1.5
ENZYMES
.
15
3.1.6
EQUIPMENT
.
16
3.1.7
SOFTWARE
.
17
3.1.8
BACTERIA
.
18
3.1.9
PLASMID
DESCRIPTION
.
19
3.2
METHODS
.
25
3.2.1
CELL
CULTURE
.
25
3.2.2
FLUORESCENCE
MICROSCOPY
.
27
3.2.3
DRUG
APPLICATION
.
28
3.2.4
DATA
ANALYSIS
.
29
3.2.5
CLONING
.
30
3.2.6
SEQUENCING
OF
PLASMIDS
.
33
4
RESULTS
.
34
4.1
CONTRIBUTION
.
34
4.2
PH(AKT)
IS
RESTRICTED
TO
THE
BASAL
75
PERCENT
OF
THE
PRIMARY
CILIUM
.
34
4.3
CILIARY
PH(AKT)
CAN
BE
MANIPULATED
BY
CHEMICALLY-INDUCIBLE
DIMERIZATION
.
36
4.4
THE
PI3K
INHIBITOR
LY294002
REDUCES
THE
RATE
OF
PIPK-INDUCED
CILIARY
FISSION
.
39
4.5
PI3K
CONTRIBUTES
TO
THE
PROCESS
OF
CILIARY
FISSION
IN
NIH
3T3
CELLS
.
42
5
DISCUSSION
.
45
5.1
DETECTION
OF
CILIARY
PI(3,4,5)P
3
USING
THE
PLECKSTRIN
HOMOLOGY
DOMAIN
OF
AKT
.
45
5.2
THE
INFLUENCE
OF
CONSTITUTIVE
EXPRESSION
AND
ACUTE
RECRUITMENT
OF
PIPK
ON
CILIARY
PI(3,4,5)P3
LEVELS
AND
STABILITY
.
48
5.3
THE
INFLUENCE
OF
CONSTITUTIVE
EXPRESSION
OF
INP54P
ON
CILIARY
PH(AKT)
LEVELS
AND
CILIARY
LENGTH
.
49
5.4
THE
INFLUENCE
OF
PI3K
CLASS
I
RECRUITMENT
ON
CILIARY
PI(3,4,5)P
3
LEVELS
AND
LENGTH.
50
5.5
ENDOGENOUS
PI3K
IS
INVOLVED
IN
THE
PROCESS
OF
CILIARY
FISSION
.
51
5.6
LIMITATIONS
AND
REMAINING
QUESTIONS
.
54
5.7
THERAPEUTIC
APPROACH
FOR
CILIOPATHIES
.
56
6
CONCLUSION
.
57
7
REFERENCES
.
58
8
ACKNOWLEDGEMENTS
.
63
9
DECLARATIONS
.
64
10
CURRICULUM
VITAE
.
66
IV |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Benninghoven-Frey, Hannah Louise 1994- |
| author_GND | (DE-588)122612447X |
| author_facet | Benninghoven-Frey, Hannah Louise 1994- |
| author_role | aut |
| author_sort | Benninghoven-Frey, Hannah Louise 1994- |
| author_variant | h l b f hlb hlbf |
| building | Verbundindex |
| bvnumber | BV047464423 |
| ctrlnum | (OCoLC)1268187891 (DE-599)DNB1236371984 |
| discipline | Medizin |
| discipline_str_mv | Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01675nam a2200397 c 4500</leader><controlfield tag="001">BV047464423</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">210913s2020 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">21,H10</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1236371984</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Broschur</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1268187891</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1236371984</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">2\p</subfield><subfield code="a">610</subfield><subfield code="2">23sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">1\p</subfield><subfield code="a">616.2</subfield><subfield code="2">23ksdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Benninghoven-Frey, Hannah Louise</subfield><subfield code="d">1994-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)122612447X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium</subfield><subfield code="c">vorgelegt von Hannah Louise Benninghoven-Frey</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Aachen</subfield><subfield code="c">[2020]</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">VIII, 67 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield><subfield code="c">21 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Rheinisch-Westfälische Technische Hochschule Aachen</subfield><subfield code="d">2020</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">https://d-nb.info/1236371984/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032866148&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-032866148</subfield></datafield><datafield tag="883" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">aepkn</subfield><subfield code="c">0,72150</subfield><subfield code="d">20210901</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#aepkn</subfield></datafield><datafield tag="883" ind1="2" ind2=" "><subfield code="8">2\p</subfield><subfield code="a">dnb</subfield><subfield code="d">20210906</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#dnb</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047464423 |
| illustrated | Illustrated |
| index_date | 2024-07-03T18:07:17Z |
| indexdate | 2024-07-10T09:12:53Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032866148 |
| oclc_num | 1268187891 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | VIII, 67 Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| record_format | marc |
| spelling | Benninghoven-Frey, Hannah Louise 1994- Verfasser (DE-588)122612447X aut The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey Aachen [2020] VIII, 67 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2020 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1236371984/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032866148&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p aepkn 0,72150 20210901 DE-101 https://d-nb.info/provenance/plan#aepkn 2\p dnb 20210906 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Benninghoven-Frey, Hannah Louise 1994- The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| subject_GND | (DE-588)4113937-9 |
| title | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_auth | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_exact_search | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_exact_search_txtP | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_full | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey |
| title_fullStr | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey |
| title_full_unstemmed | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey |
| title_short | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_sort | the role of phosphoinositides and phosphoinositide 3 kinase in the primary cilium |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1236371984/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032866148&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT benninghovenfreyhannahlouise theroleofphosphoinositidesandphosphoinositide3kinaseintheprimarycilium |
There is no print copy available.
Indexes