The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
[2020]
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | VIII, 67 Seiten Illustrationen, Diagramme 21 cm |
Internformat
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| 100 | 1 | |a Benninghoven-Frey, Hannah Louise |d 1994- |e Verfasser |0 (DE-588)122612447X |4 aut | |
| 245 | 1 | 0 | |a The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |c vorgelegt von Hannah Louise Benninghoven-Frey |
| 264 | 1 | |a Aachen |c [2020] | |
| 300 | |a VIII, 67 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Rheinisch-Westfälische Technische Hochschule Aachen |d 2020 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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Datensatz im Suchindex
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|---|---|
| adam_text | TABLE
OF
CONTENTS
ABSTRACT
.................................................................................................................................
I
ZUSAMMENFASSUNG
..............................................................................................................
II
TABLE
OF
CONTENTS
...............................................................................................................
ILL
INDEX
OF
FIGURES
...................................................................................................................
V
INDEX
OF
TABLES
...................................................................................................................
VI
LIST
OF
ABBREVIATIONS
........................................................................................................
VII
1
INTRODUCTION
................................................................................................................
1
1.1
FUNCTION
AND
STRUCTURE
OF
THE
PRIMARY
CILIUM
............................................................
1
1.2
LIFE
CYCLE
OF
THE
PRIMARY
CILIUM
..................................................................................
4
1.3
PHOSPHOINOSITIDES
IN
EUKARYOTIC
CELLS
.........................................................................
6
1.3.1
PHOSPHOINOSITIDES
IN
THE
PRIMARY
CILIUM
.................................................................
8
1.4
CILIOPATHIES
.................................................................................................................
8
1.4.1
JOUBERT
SYNDROME
...................................................................................................
9
1.5
CHEMICALLY-INDUCIBLE
DIMERIZATION
(CID)
..................................................................
10
2
AIMS
AND
OBJECTIVES
.................................................................................................
12
3
MATERIAL
AND
METHODS
..............................................................................................
13
3.1
MATERIAL
....................................................................................................................
13
3.1.1
BUFFERS,
SOLUTIONS
AND
REAGENTS
...........................................................................
13
3.1.2
MEDIA
....................................................................................................................
14
3.1.3
TRANSFECTION
REAGENT
............................................................................................
15
3.1.4
KITS
.........................................................................................................................
15
3.1.5
ENZYMES
................................................................................................................
15
3.1.6
EQUIPMENT
.............................................................................................................
16
3.1.7
SOFTWARE
................................................................................................................
17
3.1.8
BACTERIA
.................................................................................................................
18
3.1.9
PLASMID
DESCRIPTION
...............................................................................................
19
3.2
METHODS
....................................................................................................................
25
3.2.1
CELL
CULTURE
............................................................................................................
25
3.2.2
FLUORESCENCE
MICROSCOPY
......................................................................................
27
3.2.3
DRUG
APPLICATION
....................................................................................................
28
3.2.4
DATA
ANALYSIS
.........................................................................................................
29
3.2.5
CLONING
...................................................................................................................
30
3.2.6
SEQUENCING
OF
PLASMIDS
.........................................................................................
33
4
RESULTS
........................................................................................................................
34
4.1
CONTRIBUTION
..............................................................................................................
34
4.2
PH(AKT)
IS
RESTRICTED
TO
THE
BASAL
75
PERCENT
OF
THE
PRIMARY
CILIUM
........................
34
4.3
CILIARY
PH(AKT)
CAN
BE
MANIPULATED
BY
CHEMICALLY-INDUCIBLE
DIMERIZATION
...........
36
4.4
THE
PI3K
INHIBITOR
LY294002
REDUCES
THE
RATE
OF
PIPK-INDUCED
CILIARY
FISSION
.......
39
4.5
PI3K
CONTRIBUTES
TO
THE
PROCESS
OF
CILIARY
FISSION
IN
NIH
3T3
CELLS
...........................
42
5
DISCUSSION
..................................................................................................................
45
5.1
DETECTION
OF
CILIARY
PI(3,4,5)P
3
USING
THE
PLECKSTRIN
HOMOLOGY
DOMAIN
OF
AKT
.....
45
5.2
THE
INFLUENCE
OF
CONSTITUTIVE
EXPRESSION
AND
ACUTE
RECRUITMENT
OF
PIPK
ON
CILIARY
PI(3,4,5)P3
LEVELS
AND
STABILITY
...............................................................................................
48
5.3
THE
INFLUENCE
OF
CONSTITUTIVE
EXPRESSION
OF
INP54P
ON
CILIARY
PH(AKT)
LEVELS
AND
CILIARY
LENGTH
..........................................................................................................................
49
5.4
THE
INFLUENCE
OF
PI3K
CLASS
I
RECRUITMENT
ON
CILIARY
PI(3,4,5)P
3
LEVELS
AND
LENGTH..
50
5.5
ENDOGENOUS
PI3K
IS
INVOLVED
IN
THE
PROCESS
OF
CILIARY
FISSION
..................................
51
5.6
LIMITATIONS
AND
REMAINING
QUESTIONS
......................................................................
54
5.7
THERAPEUTIC
APPROACH
FOR
CILIOPATHIES
.....................................................................
56
6
CONCLUSION
.................................................................................................................
57
7
REFERENCES
.................................................................................................................
58
8
ACKNOWLEDGEMENTS
...................................................................................................
63
9
DECLARATIONS
...............................................................................................................
64
10
CURRICULUM
VITAE
...................................................................................................
66
IV
|
| adam_txt |
TABLE
OF
CONTENTS
ABSTRACT
.
I
ZUSAMMENFASSUNG
.
II
TABLE
OF
CONTENTS
.
ILL
INDEX
OF
FIGURES
.
V
INDEX
OF
TABLES
.
VI
LIST
OF
ABBREVIATIONS
.
VII
1
INTRODUCTION
.
1
1.1
FUNCTION
AND
STRUCTURE
OF
THE
PRIMARY
CILIUM
.
1
1.2
LIFE
CYCLE
OF
THE
PRIMARY
CILIUM
.
4
1.3
PHOSPHOINOSITIDES
IN
EUKARYOTIC
CELLS
.
6
1.3.1
PHOSPHOINOSITIDES
IN
THE
PRIMARY
CILIUM
.
8
1.4
CILIOPATHIES
.
8
1.4.1
JOUBERT
SYNDROME
.
9
1.5
CHEMICALLY-INDUCIBLE
DIMERIZATION
(CID)
.
10
2
AIMS
AND
OBJECTIVES
.
12
3
MATERIAL
AND
METHODS
.
13
3.1
MATERIAL
.
13
3.1.1
BUFFERS,
SOLUTIONS
AND
REAGENTS
.
13
3.1.2
MEDIA
.
14
3.1.3
TRANSFECTION
REAGENT
.
15
3.1.4
KITS
.
15
3.1.5
ENZYMES
.
15
3.1.6
EQUIPMENT
.
16
3.1.7
SOFTWARE
.
17
3.1.8
BACTERIA
.
18
3.1.9
PLASMID
DESCRIPTION
.
19
3.2
METHODS
.
25
3.2.1
CELL
CULTURE
.
25
3.2.2
FLUORESCENCE
MICROSCOPY
.
27
3.2.3
DRUG
APPLICATION
.
28
3.2.4
DATA
ANALYSIS
.
29
3.2.5
CLONING
.
30
3.2.6
SEQUENCING
OF
PLASMIDS
.
33
4
RESULTS
.
34
4.1
CONTRIBUTION
.
34
4.2
PH(AKT)
IS
RESTRICTED
TO
THE
BASAL
75
PERCENT
OF
THE
PRIMARY
CILIUM
.
34
4.3
CILIARY
PH(AKT)
CAN
BE
MANIPULATED
BY
CHEMICALLY-INDUCIBLE
DIMERIZATION
.
36
4.4
THE
PI3K
INHIBITOR
LY294002
REDUCES
THE
RATE
OF
PIPK-INDUCED
CILIARY
FISSION
.
39
4.5
PI3K
CONTRIBUTES
TO
THE
PROCESS
OF
CILIARY
FISSION
IN
NIH
3T3
CELLS
.
42
5
DISCUSSION
.
45
5.1
DETECTION
OF
CILIARY
PI(3,4,5)P
3
USING
THE
PLECKSTRIN
HOMOLOGY
DOMAIN
OF
AKT
.
45
5.2
THE
INFLUENCE
OF
CONSTITUTIVE
EXPRESSION
AND
ACUTE
RECRUITMENT
OF
PIPK
ON
CILIARY
PI(3,4,5)P3
LEVELS
AND
STABILITY
.
48
5.3
THE
INFLUENCE
OF
CONSTITUTIVE
EXPRESSION
OF
INP54P
ON
CILIARY
PH(AKT)
LEVELS
AND
CILIARY
LENGTH
.
49
5.4
THE
INFLUENCE
OF
PI3K
CLASS
I
RECRUITMENT
ON
CILIARY
PI(3,4,5)P
3
LEVELS
AND
LENGTH.
50
5.5
ENDOGENOUS
PI3K
IS
INVOLVED
IN
THE
PROCESS
OF
CILIARY
FISSION
.
51
5.6
LIMITATIONS
AND
REMAINING
QUESTIONS
.
54
5.7
THERAPEUTIC
APPROACH
FOR
CILIOPATHIES
.
56
6
CONCLUSION
.
57
7
REFERENCES
.
58
8
ACKNOWLEDGEMENTS
.
63
9
DECLARATIONS
.
64
10
CURRICULUM
VITAE
.
66
IV |
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| index_date | 2024-07-03T18:07:17Z |
| indexdate | 2024-07-10T09:12:53Z |
| institution | BVB |
| language | English |
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| physical | VIII, 67 Seiten Illustrationen, Diagramme 21 cm |
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| spelling | Benninghoven-Frey, Hannah Louise 1994- Verfasser (DE-588)122612447X aut The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey Aachen [2020] VIII, 67 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2020 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1236371984/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032866148&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p aepkn 0,72150 20210901 DE-101 https://d-nb.info/provenance/plan#aepkn 2\p dnb 20210906 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Benninghoven-Frey, Hannah Louise 1994- The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| subject_GND | (DE-588)4113937-9 |
| title | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_auth | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_exact_search | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_exact_search_txtP | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_full | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey |
| title_fullStr | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey |
| title_full_unstemmed | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey |
| title_short | The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium |
| title_sort | the role of phosphoinositides and phosphoinositide 3 kinase in the primary cilium |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1236371984/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032866148&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT benninghovenfreyhannahlouise theroleofphosphoinositidesandphosphoinositide3kinaseintheprimarycilium |
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