Engineering formatotrophic growth in Escherichia coli:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Potsdam
[2020]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | V, 107 Seiten Illustrationen 30 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV047334836 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 210617s2020 gw a||| m||| 00||| eng d | ||
| 015 | |a 21,H02 |2 dnb | ||
| 016 | 7 | |a 1223182495 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1225838186 | ||
| 035 | |a (DE-599)DNB1223182495 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 100 | 1 | |a Wenk, Sebastian |e Verfasser |0 (DE-588)1220117633 |4 aut | |
| 245 | 1 | 0 | |a Engineering formatotrophic growth in Escherichia coli |c Sebastian Wenk |
| 264 | 1 | |a Potsdam |c [2020] | |
| 300 | |a V, 107 Seiten |b Illustrationen |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Universität Potsdam |d 2020 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1223182495/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032737397&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032737397 | ||
Datensatz im Suchindex
| _version_ | 1804182547345702912 |
|---|---|
| adam_text | TABLE
OF
CONTENT
OVERARCHING
INTRODUCTION
..........................................................................................................
1
CO
2
-GREENHOUSE
GAS
AND
VALUABLE
FEEDSTOCK
...............................................................................
1
BIOLOGICAL
CO2
AND
CI
FIXATION
.........................................................................................................
2
CO2
CAPTURE
AND
CONVERSION
INTO
FORMATE
......................................................................................
4
TOWARDS
A
SUSTAINABLE
BIOECONOMY
-
SYNTHETIC
CI
ASSIMILATION
...................................................
6
SYNTHETIC
FORMATE
ASSIMILATION
PATHWAYS
.......................................................................................
7
AIM
OF
THIS
WORK
...............................................................................................................................
9
MATERIALS
.............................................................................................................................................
11
REAGENTS
........................................................................................................................................
11
GROWTH
MEDIA
................................................................................................................................
11
BACTERIAL
STRAINS
.............................................................................................................................
11
METHODS
...............................................................................................................................................
16
GENOME
ENGINEERING
.....................................................................................................................
16
SYNTHETIC
OPERON
CONSTRUCTION
.....................................................................................................
17
GROWTH
CHARACTERIZATION
IN
MICROPLATE
READERS
...........................................................................
18
ANALYSIS
OF
SUPERNATANT
BY
ION
CHROMATOGRAPHY
..........................................................................
18
13
C-LABELLING
OF
PROTEINOGENIC
AMINO
ACIDS
...................................................................................
19
DRY
WEIGHT
ANALYSIS
.................................................................
19
ENZYMES
AND
CHEMICAL
ASSAYS
.......................................................................................................
20
QUANTITATIVE
POLYMERASE
CHAIN
REACTION
......................................................................................
20
CHAPTER
1:
ESTABLISHING
A
STRATEGY
AND
METHODS
FOR
METABOLIC
ENGINEERING
OF
E.
COU
........
.
..............................
............
...21
STRAIN
ENGINEERING:
IN
VIVO
SELECTION
FOR
PATHWAY
MODULES
.........................................................
23
PATHWAY
ENGINEERING:
FINE-TUNE
EXPRESSION
OF
ENZYMES
.............................................................
25
GENOMIC
INTEGRATION
OF
SYNTHETIC
OPERONS
...................................................................................
27
GROWTH
EXPERIMENTS:
IN
VIVO
SELECTION
FOR
PATHWAY
ACTIVITY
.......................................................
29
PATHWAY
CONFIRMATION:
13
C-LABELING
OF
PROTEINOGENIC
AMINO
ACIDS
.............................................
32
CHAPTER
2:
AN
ENERGY
AUXOTROPH
E.
COU
TO
TEST
THE
IN
VIVO
ACTIVITY
OF
CI-
OXIDIZING
ENZYMES......
............................................
33
CONSTRUCTION
AND
VALIDATION
OF
AN
E.
COLI
STRAIN
AUXOTROPH
TO
REDUCING
POWER
AND
ENERGY
......
34
TESTING
FORMATE
AS
A
REDUCING
POWER
SOURCE
...............................................................................
37
TESTING
METHANOL
AS
A
REDUCING
POWER
SOURCE
.............................................................................
38
CHAPTER
3:
ESTABLISHING
THE
SERINE
THREONINE
CYCLE
IN
COLL
...................
.............
43
PRODUCTION
OF
ACETYL-COA,
GLYCINE
AND
SERINE
VIA
THREONINE
SYNTHESIS
AND
CLEAVAGE
...............
45
ESTABLISHMENT
OF
AN
EXTERNAL
ENERGY
PRODUCTION
MODULE
IMPROVES
GROWTH
ON
GLYCEROL
AND
ALLOWS
GROWTH
ON
PYRUVATE
............................................................................................................
49
FORMATE
IS
ASSIMILATED
TO
ALLOW
GROWTH
VIA
THE
SERINE
THREONINE
CYCLE
......................................
51
SHORT-TERM
EVOLUTION
IMPROVES
GROWTH
ON
PYRUVATE
AND
FORMATE
AND
ENABLES
GROWTH
ON
GLYCINE
AND
FORMATE
....................................................................................................................
54
CHAPTER
4:
GROWTH
OF
E.
COU
ON
FORMATE
AND
METHANOL
VIA
THE
REDUCTIVE
GLYCINE
PATHWAY
......................
57
THE
REDUCTIVE
GLYCINE
PATHWAY
......................................................................................................
59
MODULAR
ENGINEERING
ESTABLISHES
GROWTH
ON
FORMATE
..................................................................
61
SHORT-TERM
EVOLUTION
IMPROVES
GROWTH
ON
FORMATE
....................................................................
64
CARBON
LABELLING
SHEDS
LIGHT
ON
CELLULAR
FLUXES
.............................................................................
66
ENGINEERED
GROWTH
OF
E.
COLI
ON
METHANOL
...................................................................................
67
DISCUSSION
............................................................................................................................................
71
SYNTHETIC
BACTERIAL
METABOLISM
.....................................................................................................
71
THE
POWER
OF
ADAPTIVE
LABORATORY
EVOLUTION
...............................................................................
72
MEETING
CELLULAR
ENERGY
DEMANDS
BY
CI
OXIDATION
.......................................................................
73
FORMATOTROPHIC
GROWTH
VIA
THE
REDUCTIVE
GLYCINE
PATHWAY
.........................................................
74
FORMATOTROPHIC
GROWTH
VIA
THE
SERINE
THREONINE
CYCLE
...............................................................
75
OUTLOOK
-
THE
FUTURE
OF
SYNTHETIC
CI
METABOLISM
........................................................................
77
APPENDIX
...............................................................................................................................................
79
SUPPLEMENTARY
FIGURES
..................................................................................................................
79
REFERENCES
............................................................................................................................................
89
DISCLAIMER
..........................................................................................................................................
103
DECLARATION
OF
WORK
...................................................................................................................
105
EIDESSTATTLICHE
ERKLARUNG
........................................................................................................
105
ACKNOWLEDGEMENTS
.....................................................................................................................
107
II
|
| adam_txt |
TABLE
OF
CONTENT
OVERARCHING
INTRODUCTION
.
1
CO
2
-GREENHOUSE
GAS
AND
VALUABLE
FEEDSTOCK
.
1
BIOLOGICAL
CO2
AND
CI
FIXATION
.
2
CO2
CAPTURE
AND
CONVERSION
INTO
FORMATE
.
4
TOWARDS
A
SUSTAINABLE
BIOECONOMY
-
SYNTHETIC
CI
ASSIMILATION
.
6
SYNTHETIC
FORMATE
ASSIMILATION
PATHWAYS
.
7
AIM
OF
THIS
WORK
.
9
MATERIALS
.
11
REAGENTS
.
11
GROWTH
MEDIA
.
11
BACTERIAL
STRAINS
.
11
METHODS
.
16
GENOME
ENGINEERING
.
16
SYNTHETIC
OPERON
CONSTRUCTION
.
17
GROWTH
CHARACTERIZATION
IN
MICROPLATE
READERS
.
18
ANALYSIS
OF
SUPERNATANT
BY
ION
CHROMATOGRAPHY
.
18
13
C-LABELLING
OF
PROTEINOGENIC
AMINO
ACIDS
.
19
DRY
WEIGHT
ANALYSIS
.
19
ENZYMES
AND
CHEMICAL
ASSAYS
.
20
QUANTITATIVE
POLYMERASE
CHAIN
REACTION
.
20
CHAPTER
1:
ESTABLISHING
A
STRATEGY
AND
METHODS
FOR
METABOLIC
ENGINEERING
OF
E.
COU
.
.
.
.
.21
STRAIN
ENGINEERING:
IN
VIVO
SELECTION
FOR
PATHWAY
MODULES
.
23
PATHWAY
ENGINEERING:
FINE-TUNE
EXPRESSION
OF
ENZYMES
.
25
GENOMIC
INTEGRATION
OF
SYNTHETIC
OPERONS
.
27
GROWTH
EXPERIMENTS:
IN
VIVO
SELECTION
FOR
PATHWAY
ACTIVITY
.
29
PATHWAY
CONFIRMATION:
13
C-LABELING
OF
PROTEINOGENIC
AMINO
ACIDS
.
32
CHAPTER
2:
AN
ENERGY
AUXOTROPH
E.
COU
TO
TEST
THE
IN
VIVO
ACTIVITY
OF
CI-
OXIDIZING
ENZYMES.
.
33
CONSTRUCTION
AND
VALIDATION
OF
AN
E.
COLI
STRAIN
AUXOTROPH
TO
REDUCING
POWER
AND
ENERGY
.
34
TESTING
FORMATE
AS
A
REDUCING
POWER
SOURCE
.
37
TESTING
METHANOL
AS
A
REDUCING
POWER
SOURCE
.
38
CHAPTER
3:
ESTABLISHING
THE
SERINE
THREONINE
CYCLE
IN
COLL
.
.
43
PRODUCTION
OF
ACETYL-COA,
GLYCINE
AND
SERINE
VIA
THREONINE
SYNTHESIS
AND
CLEAVAGE
.
45
ESTABLISHMENT
OF
AN
EXTERNAL
ENERGY
PRODUCTION
MODULE
IMPROVES
GROWTH
ON
GLYCEROL
AND
ALLOWS
GROWTH
ON
PYRUVATE
.
49
FORMATE
IS
ASSIMILATED
TO
ALLOW
GROWTH
VIA
THE
SERINE
THREONINE
CYCLE
.
51
SHORT-TERM
EVOLUTION
IMPROVES
GROWTH
ON
PYRUVATE
AND
FORMATE
AND
ENABLES
GROWTH
ON
GLYCINE
AND
FORMATE
.
54
CHAPTER
4:
GROWTH
OF
E.
COU
ON
FORMATE
AND
METHANOL
VIA
THE
REDUCTIVE
GLYCINE
PATHWAY
.
57
THE
REDUCTIVE
GLYCINE
PATHWAY
.
59
MODULAR
ENGINEERING
ESTABLISHES
GROWTH
ON
FORMATE
.
61
SHORT-TERM
EVOLUTION
IMPROVES
GROWTH
ON
FORMATE
.
64
CARBON
LABELLING
SHEDS
LIGHT
ON
CELLULAR
FLUXES
.
66
ENGINEERED
GROWTH
OF
E.
COLI
ON
METHANOL
.
67
DISCUSSION
.
71
SYNTHETIC
BACTERIAL
METABOLISM
.
71
THE
POWER
OF
ADAPTIVE
LABORATORY
EVOLUTION
.
72
MEETING
CELLULAR
ENERGY
DEMANDS
BY
CI
OXIDATION
.
73
FORMATOTROPHIC
GROWTH
VIA
THE
REDUCTIVE
GLYCINE
PATHWAY
.
74
FORMATOTROPHIC
GROWTH
VIA
THE
SERINE
THREONINE
CYCLE
.
75
OUTLOOK
-
THE
FUTURE
OF
SYNTHETIC
CI
METABOLISM
.
77
APPENDIX
.
79
SUPPLEMENTARY
FIGURES
.
79
REFERENCES
.
89
DISCLAIMER
.
103
DECLARATION
OF
WORK
.
105
EIDESSTATTLICHE
ERKLARUNG
.
105
ACKNOWLEDGEMENTS
.
107
II |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Wenk, Sebastian |
| author_GND | (DE-588)1220117633 |
| author_facet | Wenk, Sebastian |
| author_role | aut |
| author_sort | Wenk, Sebastian |
| author_variant | s w sw |
| building | Verbundindex |
| bvnumber | BV047334836 |
| ctrlnum | (OCoLC)1225838186 (DE-599)DNB1223182495 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01272nam a2200337 c 4500</leader><controlfield tag="001">BV047334836</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">210617s2020 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">21,H02</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1223182495</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Broschur</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1225838186</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1223182495</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wenk, Sebastian</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1220117633</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Engineering formatotrophic growth in Escherichia coli</subfield><subfield code="c">Sebastian Wenk</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Potsdam</subfield><subfield code="c">[2020]</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">V, 107 Seiten</subfield><subfield code="b">Illustrationen</subfield><subfield code="c">30 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Universität Potsdam</subfield><subfield code="d">2020</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">https://d-nb.info/1223182495/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032737397&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-032737397</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047334836 |
| illustrated | Illustrated |
| index_date | 2024-07-03T17:32:15Z |
| indexdate | 2024-07-10T09:09:17Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032737397 |
| oclc_num | 1225838186 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | V, 107 Seiten Illustrationen 30 cm |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| record_format | marc |
| spelling | Wenk, Sebastian Verfasser (DE-588)1220117633 aut Engineering formatotrophic growth in Escherichia coli Sebastian Wenk Potsdam [2020] V, 107 Seiten Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Potsdam 2020 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1223182495/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032737397&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wenk, Sebastian Engineering formatotrophic growth in Escherichia coli |
| subject_GND | (DE-588)4113937-9 |
| title | Engineering formatotrophic growth in Escherichia coli |
| title_auth | Engineering formatotrophic growth in Escherichia coli |
| title_exact_search | Engineering formatotrophic growth in Escherichia coli |
| title_exact_search_txtP | Engineering formatotrophic growth in Escherichia coli |
| title_full | Engineering formatotrophic growth in Escherichia coli Sebastian Wenk |
| title_fullStr | Engineering formatotrophic growth in Escherichia coli Sebastian Wenk |
| title_full_unstemmed | Engineering formatotrophic growth in Escherichia coli Sebastian Wenk |
| title_short | Engineering formatotrophic growth in Escherichia coli |
| title_sort | engineering formatotrophic growth in escherichia coli |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1223182495/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032737397&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wenksebastian engineeringformatotrophicgrowthinescherichiacoli |
Es ist kein Print-Exemplar vorhanden.
Inhaltsverzeichnis