Tracking self-assembly of hierarchically structured soft matter:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Potsdam
[2019]
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | ix, 88 Seiten Illustrationen 30 cm |
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MARC
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| 245 | 1 | 0 | |a Tracking self-assembly of hierarchically structured soft matter |c von Franziska Jehle |
| 264 | 1 | |a Potsdam |c [2019] | |
| 300 | |a ix, 88 Seiten |b Illustrationen |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Universität Potsdam |d 2019 | ||
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Record in the Search Index
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| adam_text | TABLE
OF
CONTENTS
ABSTRACT
................................................................................................................................................
III
TABLE
OF
CONTENTS
.............................................................................................................................
VI
LIST
OF
FIGURES
...................................................................................................................................
VIII
LIST
OF
ABBREVIATIONS.........................................................................................................................
X
1.
INTRODUCTION
..............................................................................................................................
1
2.
MOTIVATION
&
AIMS
....................................................................................................................
5
3.
STATE
OF
THE
ART
.........................................................................................................................
7
3.2
STRUCTURE-FUNCTION
RELATIONSHIP
...........................................................................................8
3.2.1
THE
PLAQUE
-
A
MULTIFACETED
UNDERWATER
ADHESIVE
.....................................................8
3.2.2
THE
CORE
-
A
SELF-HEALING
GRADED
BLOCK
COPOLYMER
...................................................
11
3.2.3
THE
CUTICLE
-
A
PROTECTIVE
REINFORCED
COMPOSITE
......................................................
12
3.3
ESTABLISHED
METHODS
FOR
BYSSUS
EXAMINATION
................................................................18
3.4
MUSSEL-INSPIRED
MATERIALS
.................................................................................................
20
3.4.1
MATERIALS
FROM
EXTRACTED
BYSSUS
PROTEINS
..................................................................
20
3.4.2
MATERIALS
FROM
RECOMBINANT
BYSSUS
PROTEINS
............................................................
21
3.4.3
MATERIALS
FROM
BYSSUS
PEPTIDES
...................................................................................
23
4.
METAL-TUNABLE
SELF-ASSEMBLY
OF
HIERARCHICAL
STRUCTURE
IN
MUSSEL-INSPIRED
PEPTIDE
FILMS..................................................................................................................................
26
4.1
MATERIAL
&
METHODS
...........................................................................................................
28
4.1.1
FILM
FORMATION
................................................................................................................
28
4.1.2
FOURIER
TRANSFORM
INFRARED
SPECTROSCOPY
...................................................................
28
4.1.3
RAMAN
SPECTROSCOPY
......................................................................................................
28
4.1.4
X-RAY
ABSORPTION
NEAR
EDGE
SPECTROSCOPY
(XANES)
..................................................
29
4.1.5
X-RAY
DIFFRACTION
..............................................................................................................
29
4.1.6
AFM-BASED
INDENTATION
................................................................................................
29
4.2
RESULTS
..................................................................................................................................
30
4.2.1
SEQUENCE
SELECTION
AND
FILM
FORMATION
.....................................................................
30
4.2.2
FOURIER
TRANSFORM
INFRARED
(FTIR)
AND
RAMAN
SPECTROSCOPY
..................................
30
4.2.3
X-RAY
ABSORPTION
SPECTROSCOPY
(XAS)..........................................................................
32
4.2.4
POLARIZED
RAMAN
SPECTROSCOPY
....................................................................................
33
4.2.5
X-RAY
DIFFRACTION
.............................................................................................................
34
4.2.6
MECHANICAL
CHARACTERIZATION
........................................................................................
37
4.3
DISCUSSION
.............................................................................................................................
39
VI
4.4
CONCLUSION
...........................................................................................................................
41
5.
ULTRASTRUCTURE
AND
COMPOSITION
OF
BYSSAL
THREAD
CUTICLE
.................................
42
5.1
MATERIAL
&
METHODS
.........................................................................................................
44
5.1.1
CHEMICAL
FIXATION
&
EMBEDDING
...................................................................................
44
5.1.2
TEM-EDS
..........................................................................................................................
44
5.1.3
FIB-SEM
.........................................................................................................................
44
5.1.4
IMAGE
PROCESSING
...........................................................................................................
45
5.1.5
SEGMENTATION
.................................................................................................................
45
5.1.6
VOLUME
FRACTIONS
AND
3D
THICKNESS
MAPPING
............................................................
45
5.2
RESULTS
..................................................................................................................................
46
5.2.1
ULTRASTRUCTURE
OF
M.
EDULIS
BYSSAL
THREAD
CUTICLE
.......................................................
46
5.2.2
ELEMENTAL
COMPOSITION
OF
BYSSAL
THREAD
CUTICLE
........................................................
47
5.2.3
3D-RECONSTRUCTION
OF
CUTICLE
GRANULES
.........................................................................
49
5.3
DISCUSSION
............................................................................................................................
55
5.4
CONCLUSION
...........................................................................................................................
59
6.
DYNAMIC
FORMATION
PROCESS
OF
BYSSAL
THREAD
CUTICLE
............................................
60
6.1
MATERIAL
&
METHODS
...........................................................................................................61
6.1.1
CHEMICAL
FIXATION
AND
EMBEDDING
...............................................................................61
6.1.2
INDUCED
SECRETION
AND
THREAD
FORMATION
....................................................................62
6.1.3
SAMPLE
PREPARATION
FOR
TEM
........................................................................................
62
6.1.4
TEM-EDS
..........................................................................................................................62
6.1.5
FIB-SEM
...........................................................................................................................63
6.1.6
IMAGE
PROCESSING
...........................................................................................................63
6.1.7
SEGMENTATION
..................................................................................................................63
6.2
RESULTS
..................................................................................................................................
64
6.2.1
DEVELOPMENT
OF
A
FIXATION
PROTOCOL
SUITABLE
FOR
MUSSEL
FOOT
TISSUE
......................
64
6.2.2
ULTRASTRUCTURE
OF
CUTICLE
VESICLES
IN
GLAND
TISSUE
.......................................................
65
6.2.3
ELEMENTAL
COMPOSITION
OF
CUTICLE
VESICLES
..................................................................
67
6.2.4
3D-RECONSTRUCTION
OF
VESICLE
PHASES
...........................................................................
70
6.2.5
INDUCED
THREAD
FORMATION
.............................................................................................
72
6.3
DISCUSSION
............................................................................................................................
75
6.4
CONCLUSION
...........................................................................................................................
77
7.
REFERENCES
.................................................................................................................................79
8.
DECLARATION
OF
ORIGINALITY
/
EIDESSTATTLICHE
ERKLARUNG........................................
87
9.
ACKNOWLEDGEMENTS
..............................................................................................................
88
VII
|
| adam_txt |
TABLE
OF
CONTENTS
ABSTRACT
.
III
TABLE
OF
CONTENTS
.
VI
LIST
OF
FIGURES
.
VIII
LIST
OF
ABBREVIATIONS.
X
1.
INTRODUCTION
.
1
2.
MOTIVATION
&
AIMS
.
5
3.
STATE
OF
THE
ART
.
7
3.2
STRUCTURE-FUNCTION
RELATIONSHIP
.8
3.2.1
THE
PLAQUE
-
A
MULTIFACETED
UNDERWATER
ADHESIVE
.8
3.2.2
THE
CORE
-
A
SELF-HEALING
GRADED
BLOCK
COPOLYMER
.
11
3.2.3
THE
CUTICLE
-
A
PROTECTIVE
REINFORCED
COMPOSITE
.
12
3.3
ESTABLISHED
METHODS
FOR
BYSSUS
EXAMINATION
.18
3.4
MUSSEL-INSPIRED
MATERIALS
.
20
3.4.1
MATERIALS
FROM
EXTRACTED
BYSSUS
PROTEINS
.
20
3.4.2
MATERIALS
FROM
RECOMBINANT
BYSSUS
PROTEINS
.
21
3.4.3
MATERIALS
FROM
BYSSUS
PEPTIDES
.
23
4.
METAL-TUNABLE
SELF-ASSEMBLY
OF
HIERARCHICAL
STRUCTURE
IN
MUSSEL-INSPIRED
PEPTIDE
FILMS.
26
4.1
MATERIAL
&
METHODS
.
28
4.1.1
FILM
FORMATION
.
28
4.1.2
FOURIER
TRANSFORM
INFRARED
SPECTROSCOPY
.
28
4.1.3
RAMAN
SPECTROSCOPY
.
28
4.1.4
X-RAY
ABSORPTION
NEAR
EDGE
SPECTROSCOPY
(XANES)
.
29
4.1.5
X-RAY
DIFFRACTION
.
29
4.1.6
AFM-BASED
INDENTATION
.
29
4.2
RESULTS
.
30
4.2.1
SEQUENCE
SELECTION
AND
FILM
FORMATION
.
30
4.2.2
FOURIER
TRANSFORM
INFRARED
(FTIR)
AND
RAMAN
SPECTROSCOPY
.
30
4.2.3
X-RAY
ABSORPTION
SPECTROSCOPY
(XAS).
32
4.2.4
POLARIZED
RAMAN
SPECTROSCOPY
.
33
4.2.5
X-RAY
DIFFRACTION
.
34
4.2.6
MECHANICAL
CHARACTERIZATION
.
37
4.3
DISCUSSION
.
39
VI
4.4
CONCLUSION
.
41
5.
ULTRASTRUCTURE
AND
COMPOSITION
OF
BYSSAL
THREAD
CUTICLE
.
42
5.1
MATERIAL
&
METHODS
.
44
5.1.1
CHEMICAL
FIXATION
&
EMBEDDING
.
44
5.1.2
TEM-EDS
.
44
5.1.3
FIB-SEM
.
44
5.1.4
IMAGE
PROCESSING
.
45
5.1.5
SEGMENTATION
.
45
5.1.6
VOLUME
FRACTIONS
AND
3D
THICKNESS
MAPPING
.
45
5.2
RESULTS
.
46
5.2.1
ULTRASTRUCTURE
OF
M.
EDULIS
BYSSAL
THREAD
CUTICLE
.
46
5.2.2
ELEMENTAL
COMPOSITION
OF
BYSSAL
THREAD
CUTICLE
.
47
5.2.3
3D-RECONSTRUCTION
OF
CUTICLE
GRANULES
.
49
5.3
DISCUSSION
.
55
5.4
CONCLUSION
.
59
6.
DYNAMIC
FORMATION
PROCESS
OF
BYSSAL
THREAD
CUTICLE
.
60
6.1
MATERIAL
&
METHODS
.61
6.1.1
CHEMICAL
FIXATION
AND
EMBEDDING
.61
6.1.2
INDUCED
SECRETION
AND
THREAD
FORMATION
.62
6.1.3
SAMPLE
PREPARATION
FOR
TEM
.
62
6.1.4
TEM-EDS
.62
6.1.5
FIB-SEM
.63
6.1.6
IMAGE
PROCESSING
.63
6.1.7
SEGMENTATION
.63
6.2
RESULTS
.
64
6.2.1
DEVELOPMENT
OF
A
FIXATION
PROTOCOL
SUITABLE
FOR
MUSSEL
FOOT
TISSUE
.
64
6.2.2
ULTRASTRUCTURE
OF
CUTICLE
VESICLES
IN
GLAND
TISSUE
.
65
6.2.3
ELEMENTAL
COMPOSITION
OF
CUTICLE
VESICLES
.
67
6.2.4
3D-RECONSTRUCTION
OF
VESICLE
PHASES
.
70
6.2.5
INDUCED
THREAD
FORMATION
.
72
6.3
DISCUSSION
.
75
6.4
CONCLUSION
.
77
7.
REFERENCES
.79
8.
DECLARATION
OF
ORIGINALITY
/
EIDESSTATTLICHE
ERKLARUNG.
87
9.
ACKNOWLEDGEMENTS
.
88
VII |
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| author | Jehle, Franziska |
| author_GND | (DE-588)119076380X |
| author_facet | Jehle, Franziska |
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| author_sort | Jehle, Franziska |
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| building | Verbundindex |
| bvnumber | BV047334831 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047334831 |
| illustrated | Illustrated |
| index_date | 2024-07-03T17:32:14Z |
| indexdate | 2024-07-10T09:09:17Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032737390 |
| oclc_num | 1158573962 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | ix, 88 Seiten Illustrationen 30 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spelling | Jehle, Franziska Verfasser (DE-588)119076380X aut Tracking self-assembly of hierarchically structured soft matter von Franziska Jehle Potsdam [2019] ix, 88 Seiten Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Potsdam 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1191201694/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032737390&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Jehle, Franziska Tracking self-assembly of hierarchically structured soft matter |
| subject_GND | (DE-588)4113937-9 |
| title | Tracking self-assembly of hierarchically structured soft matter |
| title_auth | Tracking self-assembly of hierarchically structured soft matter |
| title_exact_search | Tracking self-assembly of hierarchically structured soft matter |
| title_exact_search_txtP | Tracking self-assembly of hierarchically structured soft matter |
| title_full | Tracking self-assembly of hierarchically structured soft matter von Franziska Jehle |
| title_fullStr | Tracking self-assembly of hierarchically structured soft matter von Franziska Jehle |
| title_full_unstemmed | Tracking self-assembly of hierarchically structured soft matter von Franziska Jehle |
| title_short | Tracking self-assembly of hierarchically structured soft matter |
| title_sort | tracking self assembly of hierarchically structured soft matter |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1191201694/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032737390&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT jehlefranziska trackingselfassemblyofhierarchicallystructuredsoftmatter |
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