Confocal microscopy: methods and protocols
This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written i...
Gespeichert in:
| Weitere Verfasser: | , |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2021]
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| Schriftenreihe: | Methods in Molecular Biology
2304 |
| Schlagworte: | |
| Zusammenfassung: | This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Beschreibung: | This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field 1. Advances in Confocal Microscopy and Selected Applications; W. Matt Reilly and Christopher J. Obara; ; 2. Choosing Fluorescent Probes and Labeling Systems ; Jacoby Morris and George H. Patterson; ; 3. General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy; Joseph A. Brzostowski; ; 4. Microfabricated devices for confocal microscopy on biological samples; Nicole Y. Morgan; ; 5. Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging; Xufeng Wu and John A. Hammer; ; 6. High-resolution multicolor imaging of mitochondria in lymphocytes; Munir Akkaya, Pietro Miozzo, and Margery G. Smelkinson; ; 7. Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy; Paul Tillberg; ; 8. Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy ; Abhijit Ashok Ambegaonkar and Haewon Sohn; ; 9. - Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight ; Sara Hernández-Pérez, Marika Vainio, Vid Šuštar, and Pieta K. Mattila - Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy; Billur Akkaya, Olena Kamenyeva, Juraj Kabat, and Ryan Kissinger; ; 14. Studying Neuronal Biology Using Spinning Disc Confocal Microscopy; Javier Manzella-Lapeira, Joseph Brzostowski, and Jenny Serra-Vinardell ; ; 15. Method for Acute Intravital Imaging of the Large Intestine in Live Mice; Erreni Marco, Doni Andrea and Roberto Weigert; ; 16. Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism; Javier Manzella-Lapeira and Joseph Brzostowski; ; 17. Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry; Greg Alspaugh, Branden Roarke, Alexandra Chand, Rozhin Penjweini, Alessio Andreoni, and Jay R. Knutson; ; 18. FLIM Imaging for Metabolic Studies in Live Cells; Heejun Choi; ; 19. - A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM) ; Alexander Zhovmer and Christian A. Combs |
| Beschreibung: | xi, 363 Seiten Illustrationen, Daigramme 903 grams |
| ISBN: | 9781071614013 |
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| 500 | |a - A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM) ; Alexander Zhovmer and Christian A. Combs | ||
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| isbn | 9781071614013 |
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| spelling | Confocal microscopy methods and protocols edited by Joseph Brzostowski and Haewon Sohn (NAID, National Institutes of Health, Rockville, MD, USA) New York, NY Humana Press [2021] xi, 363 Seiten Illustrationen, Daigramme 903 grams txt rdacontent n rdamedia nc rdacarrier Methods in Molecular Biology 2304 This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field 1. Advances in Confocal Microscopy and Selected Applications; W. Matt Reilly and Christopher J. Obara; ; 2. Choosing Fluorescent Probes and Labeling Systems ; Jacoby Morris and George H. Patterson; ; 3. General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy; Joseph A. Brzostowski; ; 4. Microfabricated devices for confocal microscopy on biological samples; Nicole Y. Morgan; ; 5. Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging; Xufeng Wu and John A. Hammer; ; 6. High-resolution multicolor imaging of mitochondria in lymphocytes; Munir Akkaya, Pietro Miozzo, and Margery G. Smelkinson; ; 7. Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy; Paul Tillberg; ; 8. Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy ; Abhijit Ashok Ambegaonkar and Haewon Sohn; ; 9. - Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight ; Sara Hernández-Pérez, Marika Vainio, Vid Šuštar, and Pieta K. Mattila - Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy; Billur Akkaya, Olena Kamenyeva, Juraj Kabat, and Ryan Kissinger; ; 14. Studying Neuronal Biology Using Spinning Disc Confocal Microscopy; Javier Manzella-Lapeira, Joseph Brzostowski, and Jenny Serra-Vinardell ; ; 15. Method for Acute Intravital Imaging of the Large Intestine in Live Mice; Erreni Marco, Doni Andrea and Roberto Weigert; ; 16. Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism; Javier Manzella-Lapeira and Joseph Brzostowski; ; 17. Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry; Greg Alspaugh, Branden Roarke, Alexandra Chand, Rozhin Penjweini, Alessio Andreoni, and Jay R. Knutson; ; 18. FLIM Imaging for Metabolic Studies in Live Cells; Heejun Choi; ; 19. - A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM) ; Alexander Zhovmer and Christian A. Combs bicssc bisacsh Microscopy Spectroscopy Cell biology Life sciences Konfokale Mikroskopie (DE-588)4336446-9 gnd rswk-swf Allgemeines, Lexika Konfokale Mikroskopie (DE-588)4336446-9 s DE-604 Brzostowski, Joseph edt Sohn, Haewon edt Erscheint auch als Online-Ausgabe 978-1-07-161402-0 Methods in Molecular Biology 2304 (DE-604)BV035362695 2304 |
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| title | Confocal microscopy methods and protocols |
| title_auth | Confocal microscopy methods and protocols |
| title_exact_search | Confocal microscopy methods and protocols |
| title_exact_search_txtP | Confocal microscopy methods and protocols |
| title_full | Confocal microscopy methods and protocols edited by Joseph Brzostowski and Haewon Sohn (NAID, National Institutes of Health, Rockville, MD, USA) |
| title_fullStr | Confocal microscopy methods and protocols edited by Joseph Brzostowski and Haewon Sohn (NAID, National Institutes of Health, Rockville, MD, USA) |
| title_full_unstemmed | Confocal microscopy methods and protocols edited by Joseph Brzostowski and Haewon Sohn (NAID, National Institutes of Health, Rockville, MD, USA) |
| title_short | Confocal microscopy |
| title_sort | confocal microscopy methods and protocols |
| title_sub | methods and protocols |
| topic | bicssc bisacsh Microscopy Spectroscopy Cell biology Life sciences Konfokale Mikroskopie (DE-588)4336446-9 gnd |
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