The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins:
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| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Köln
[2020]
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | X, 163 Blatt Illustrationen 21 cm |
Internformat
MARC
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| 100 | 1 | |a Marx, Marie-Charlotte |d 1987- |e Verfasser |0 (DE-588)1217282017 |4 aut | |
| 245 | 1 | 0 | |a The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins |c vorgelegt von Marie-Charlotte Marx |
| 264 | 1 | |a Köln |c [2020] | |
| 300 | |a X, 163 Blatt |b Illustrationen |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 502 | |b Dissertation |c Universität zu Köln |d 2020 | ||
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENT
CONTENT
I
LIST
OF
FIGURES
V
LIST
OF
TABLES
VI
LIST
OF
SUPPLEMENT
FIGURES
VI
LIST
OF
SUPPLEMENT
TABLES
______________________________________
VII
LIST
OF
ABBREVIATIONS
___________________________________________
VIII
ABSTRACT
_________________________________________________________
1
ZUSAMMENFASSUNG
________________________________________________
2
1
INTRODUCTION
___________________________________________________
3
1.1
M
ITOCHONDRIA
3
1.1.1
M
ITOCHONDRIAL
EVOLUTION
3
1.1.2
M
ITOCHONDRIAL
FUNCTION
4
1.1.3
M
ITOCHONDRIAL
STRESS
RESPONSE
6
1.1.4
N
UCLEAR
ENCODED
MITOCHONDRIAL
PROTEINS
(NEMP
S
)
7
1.2
C
YTOPLASMIC
TRANSLATION
14
1.2.1
R
EGULATORY
STAGE
OF
TRANSLATION
INITIATION
14
1.2.2
T
HE
MODEL
OF
TRANSLATION
INITIATION
OF
E
IF4E-
BOUND
M
RNA
S
15
1.2.3
T
RANSLATION
ELONGATION
16
LIST
OF
FIGURES
AND
TABLES
1.2.4
M
TORCI
SIGNALING
PATHWAY
16
1.3
RN
A
GRANULES
19
1.3.1
RNA
GRANULE
DEFINITION
19
1.3.2
S
TRESS
GRANULES
20
1.3.3
P
ROCESSING
BODIES
22
1.4
T
HE
RNA
BINDING
PROTEIN
CLUH
23
1.4.1
H
ISTORY
OF
THE
ORTHOLOGS
OF
THE
C
LUSTERING
MITOCHONDRIA
H
OMOLOG
(CLUH)
AND
THE
PHENOTYPE
OF
DELETION
MUTANTS
23
1.4.2
CLU
ASSOCIATION
TO
THE
ER
25
1.4.3
T
HE
LINK
OF
C
LU
TO
THE
MITOPHAGY
PATHWAY
26
1.4.4
M
AMMALIAN
CLU
HOMOLOG
,
CLUH 27
1.4.5
CLUH
HOMOLOGS
CELLULAR
LOCALIZATION
30
1.4.7
CLUHSLINK
TO
RNA
GRANULES
32
1.5
A
IM
OF
THIS
WORK
33
2.
MATERIALS
AND
METHODS
35
2.1
M
ATERIALS
35
2.1.1.
P
LASMIDS
USED
FOR
OVEREXPRESSION
35
2.1.2.
A
NTIBODIES
35
2.1.3.
Q
RT-PCR
PRIMERS
37
2.1.4.
D
RUGS
37
2.2
M
ETHODS
38
II
LIST
OF
FIGURES
AND
TABLES
2.2.1
C
ELL
CULTURE
38
2.2.2
T
RANSFECTION
38
2.2.3
I
MMUNOFLUORESCENCE
39
2.2.4
L
YSATE
PREPARATION
40
2.2.5
SDS-PAGE
AND
WESTERN
BLOT
40
2.2.6
P
OLYSOME
P
ROFILING
41
2.2.7
RNA
EXTRACTION,
CDNA
SYNTHESIS
AND
QRT-PCR
41
2.2.8
I
MMUNOPRECIPITATION
42
2.2.9
M
ASS
S
PECTROMETRY
43
2.2.10
Q
UANTIFICATIONS
AND
STATISTICS
43
3.
RESULTS_____________________________________________________
44
3.1
CLUH
IS
INVOLVED
IN
TRANSLATION
OF
ITS
TARGET
M
RN
A
S
.
44
3.2
CLUH
LOCALIZES
TO
MRNPS
AND
ACTIVELY
TRANSLATED
MRNAS
IN
POLYSOME
PROHUNG
48
3.3
CLUH
RESIDES
TO
GRANULAR
STRUCTURES
UNDER
MITOCHONDRIAL
STRESS
51
3.4
CLUH
INTERACTOME
IN
HUMAN
CELLS
63
3.4.1
CLUH
IP
EXHIBITS
MITOCHONDRIAL
PROTEINS,
INCLUDING
PROTEINS
OF
CLUH
MRNA
TARGETS
63
3.4.2
CLUH
INTERACTS
WITH
PROTEINS
OF
THE
ER
67
3.4.3
CLUH
INTERACTS
WITH
COMPONENTS
OF
THE
MTORC
SIGNALING
PATHWAY
70
3.4.2
CLUH
INTERACTS
WITH
PROTEINS
INVOLVED
IN
MITOSIS
AND
THE
CYTOSKELETON
NETWORK
74
III
LIST
OF
FIGURES
AND
TABLES
3.5
I
NSULIN
MEDIATED
M
TOR
ACTIVATION
INDUCES
CLUH
GRANULE
FORMATION
DEPENDENT
ON
CLUH 79
3.6
CLUH
GRANULE
DYNAMICS
IS
AFFECTED
BY
LACK
OF
ASTRIN
84
4.
DISCUSSION___________________________________________________88
4.1
CLUH
AS
A
TRANSLATIONAL
REGULATOR
OF
ITS
TARGET
MRNAS
88
4.2
CLUH
FORMS
PERIPHERAL
GRANULES
93
4.3
CLUH
FORMS
GRANULES
UNDER
MITOCHONDRIAL
STRESS
AND
MTOR
ACTIVATION
96
4.5
C
ONCLUSION
102
§.
REFERENCES
W3
6.
APPENDIX
128
ACKNOWLEDGEMENT
162
EIDESSTATTLICHE
ERKLARUNG
163
IV
|
| adam_txt |
CONTENT
CONTENT
I
LIST
OF
FIGURES
V
LIST
OF
TABLES
VI
LIST
OF
SUPPLEMENT
FIGURES
VI
LIST
OF
SUPPLEMENT
TABLES
_
VII
LIST
OF
ABBREVIATIONS
_
VIII
ABSTRACT
_
1
ZUSAMMENFASSUNG
_
2
1
INTRODUCTION
_
3
1.1
M
ITOCHONDRIA
3
1.1.1
M
ITOCHONDRIAL
EVOLUTION
3
1.1.2
M
ITOCHONDRIAL
FUNCTION
4
1.1.3
M
ITOCHONDRIAL
STRESS
RESPONSE
6
1.1.4
N
UCLEAR
ENCODED
MITOCHONDRIAL
PROTEINS
(NEMP
S
)
7
1.2
C
YTOPLASMIC
TRANSLATION
14
1.2.1
R
EGULATORY
STAGE
OF
TRANSLATION
INITIATION
14
1.2.2
T
HE
MODEL
OF
TRANSLATION
INITIATION
OF
E
IF4E-
BOUND
M
RNA
S
15
1.2.3
T
RANSLATION
ELONGATION
16
LIST
OF
FIGURES
AND
TABLES
1.2.4
M
TORCI
SIGNALING
PATHWAY
16
1.3
RN
A
GRANULES
19
1.3.1
RNA
GRANULE
DEFINITION
19
1.3.2
S
TRESS
GRANULES
20
1.3.3
P
ROCESSING
BODIES
22
1.4
T
HE
RNA
BINDING
PROTEIN
CLUH
23
1.4.1
H
ISTORY
OF
THE
ORTHOLOGS
OF
THE
C
LUSTERING
MITOCHONDRIA
H
OMOLOG
(CLUH)
AND
THE
PHENOTYPE
OF
DELETION
MUTANTS
23
1.4.2
CLU
ASSOCIATION
TO
THE
ER
25
1.4.3
T
HE
LINK
OF
C
LU
TO
THE
MITOPHAGY
PATHWAY
26
1.4.4
M
AMMALIAN
CLU
HOMOLOG
,
CLUH 27
1.4.5
CLUH
HOMOLOGS
CELLULAR
LOCALIZATION
30
1.4.7
CLUHSLINK
TO
RNA
GRANULES
32
1.5
A
IM
OF
THIS
WORK
33
2.
MATERIALS
AND
METHODS
35
2.1
M
ATERIALS
35
2.1.1.
P
LASMIDS
USED
FOR
OVEREXPRESSION
35
2.1.2.
A
NTIBODIES
35
2.1.3.
Q
RT-PCR
PRIMERS
37
2.1.4.
D
RUGS
37
2.2
M
ETHODS
38
II
LIST
OF
FIGURES
AND
TABLES
2.2.1
C
ELL
CULTURE
38
2.2.2
T
RANSFECTION
38
2.2.3
I
MMUNOFLUORESCENCE
39
2.2.4
L
YSATE
PREPARATION
40
2.2.5
SDS-PAGE
AND
WESTERN
BLOT
40
2.2.6
P
OLYSOME
P
ROFILING
41
2.2.7
RNA
EXTRACTION,
CDNA
SYNTHESIS
AND
QRT-PCR
41
2.2.8
I
MMUNOPRECIPITATION
42
2.2.9
M
ASS
S
PECTROMETRY
43
2.2.10
Q
UANTIFICATIONS
AND
STATISTICS
43
3.
RESULTS_
44
3.1
CLUH
IS
INVOLVED
IN
TRANSLATION
OF
ITS
TARGET
M
RN
A
S
.
44
3.2
CLUH
LOCALIZES
TO
MRNPS
AND
ACTIVELY
TRANSLATED
MRNAS
IN
POLYSOME
PROHUNG
48
3.3
CLUH
RESIDES
TO
GRANULAR
STRUCTURES
UNDER
MITOCHONDRIAL
STRESS
51
3.4
CLUH
INTERACTOME
IN
HUMAN
CELLS
63
3.4.1
CLUH
IP
EXHIBITS
MITOCHONDRIAL
PROTEINS,
INCLUDING
PROTEINS
OF
CLUH
MRNA
TARGETS
63
3.4.2
CLUH
INTERACTS
WITH
PROTEINS
OF
THE
ER
67
3.4.3
CLUH
INTERACTS
WITH
COMPONENTS
OF
THE
MTORC
SIGNALING
PATHWAY
70
3.4.2
CLUH
INTERACTS
WITH
PROTEINS
INVOLVED
IN
MITOSIS
AND
THE
CYTOSKELETON
NETWORK
74
III
LIST
OF
FIGURES
AND
TABLES
3.5
I
NSULIN
MEDIATED
M
TOR
ACTIVATION
INDUCES
CLUH
GRANULE
FORMATION
DEPENDENT
ON
CLUH 79
3.6
CLUH
GRANULE
DYNAMICS
IS
AFFECTED
BY
LACK
OF
ASTRIN
84
4.
DISCUSSION_88
4.1
CLUH
AS
A
TRANSLATIONAL
REGULATOR
OF
ITS
TARGET
MRNAS
88
4.2
CLUH
FORMS
PERIPHERAL
GRANULES
93
4.3
CLUH
FORMS
GRANULES
UNDER
MITOCHONDRIAL
STRESS
AND
MTOR
ACTIVATION
96
4.5
C
ONCLUSION
102
§.
REFERENCES
W3
6.
APPENDIX
128
ACKNOWLEDGEMENT
162
EIDESSTATTLICHE
ERKLARUNG
163
IV |
| any_adam_object | 1 |
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| author | Marx, Marie-Charlotte 1987- |
| author_GND | (DE-588)1217282017 |
| author_facet | Marx, Marie-Charlotte 1987- |
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| building | Verbundindex |
| bvnumber | BV047320634 |
| ctrlnum | (OCoLC)1249690830 (DE-599)DNB1222596806 |
| discipline | Biologie |
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| id | DE-604.BV047320634 |
| illustrated | Illustrated |
| index_date | 2024-07-03T17:29:05Z |
| indexdate | 2024-07-10T09:08:49Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032723395 |
| oclc_num | 1249690830 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | X, 163 Blatt Illustrationen 21 cm |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| record_format | marc |
| spelling | Marx, Marie-Charlotte 1987- Verfasser (DE-588)1217282017 aut The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins vorgelegt von Marie-Charlotte Marx Köln [2020] X, 163 Blatt Illustrationen 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität zu Köln 2020 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1222596806/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032723395&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Marx, Marie-Charlotte 1987- The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins |
| subject_GND | (DE-588)4113937-9 |
| title | The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins |
| title_auth | The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins |
| title_exact_search | The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins |
| title_exact_search_txtP | The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins |
| title_full | The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins vorgelegt von Marie-Charlotte Marx |
| title_fullStr | The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins vorgelegt von Marie-Charlotte Marx |
| title_full_unstemmed | The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins vorgelegt von Marie-Charlotte Marx |
| title_short | The role of CLUH in regulating translation of mRNAs encoding mitochondrial proteins |
| title_sort | the role of cluh in regulating translation of mrnas encoding mitochondrial proteins |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1222596806/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032723395&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT marxmariecharlotte theroleofcluhinregulatingtranslationofmrnasencodingmitochondrialproteins |
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