DNA Electrophoresis: Methods and Protocols
This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electropho...
Gespeichert in:
Format: | Elektronisch E-Book |
---|---|
Sprache: | English |
Veröffentlicht: |
New York, NY
Springer US
2020
|
Ausgabe: | 1st ed. 2020 |
Schriftenreihe: | Methods in Molecular Biology
2119 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field |
Beschreibung: | Introduction and Perspectives of DNA Electrophoresis -- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers -- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage -- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast -- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures -- Alkali Comet Assay in Genotoxicity Tests -- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay -- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis -- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells -- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria -- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE) -- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast -- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes -- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System -- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells -- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation -- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP) -- Post-Labeling/PAGE Method for Detection of DNA Adducts |
ISBN: | 9781071603239 |
DOI: | 10.1007/978-1-0716-0323-9 |
Internformat
MARC
LEADER | 00000nmm a2200000zcb4500 | ||
---|---|---|---|
001 | BV047302585 | ||
003 | DE-604 | ||
005 | 00000000000000.0 | ||
007 | cr|uuu---uuuuu | ||
008 | 210528s2020 |||| o||u| ||||||eng d | ||
020 | |a 9781071603239 |9 978-1-07-160323-9 | ||
024 | 7 | |a 10.1007/978-1-0716-0323-9 |2 doi | |
035 | |a (ZDB-2-PRO)978-1-0716-0323-9 | ||
035 | |a (OCoLC)1256420033 | ||
035 | |a (DE-599)BVBBV047302585 | ||
040 | |a DE-604 |b ger |e rda | ||
041 | 0 | |a eng | |
049 | |a DE-355 |a DE-91 | ||
082 | 0 | |a 611.01816 | |
082 | 0 | |a 599.935 | |
245 | 1 | 0 | |a DNA Electrophoresis |b Methods and Protocols |c edited by Katsuhiro Hanada |
250 | |a 1st ed. 2020 | ||
264 | 1 | |a New York, NY |b Springer US |c 2020 | |
336 | |b txt |2 rdacontent | ||
337 | |b c |2 rdamedia | ||
338 | |b cr |2 rdacarrier | ||
490 | 0 | |a Methods in Molecular Biology |v 2119 | |
500 | |a Introduction and Perspectives of DNA Electrophoresis -- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers -- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage -- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast -- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures -- Alkali Comet Assay in Genotoxicity Tests -- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay -- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis -- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells -- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria -- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE) -- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast -- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes -- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System -- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells -- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation -- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP) -- Post-Labeling/PAGE Method for Detection of DNA Adducts | ||
520 | |a This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field | ||
650 | 4 | |a Human genetics | |
650 | 4 | |a Biomedical engineering | |
700 | 1 | |a Hanada, Katsuhiro |e Sonstige |4 oth | |
776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781071603222 |
776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781071603246 |
776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781071603253 |
856 | 4 | 0 | |u https://doi.org/10.1007/978-1-0716-0323-9 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
912 | |a ZDB-2-PRO | ||
999 | |a oai:aleph.bib-bvb.de:BVB01-032705725 | ||
347 | |a text file |b PDF | ||
966 | e | |u https://doi.org/10.1007/978-1-0716-0323-9 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
966 | e | |u https://doi.org/10.1007/978-1-0716-0323-9 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext |
Datensatz im Suchindex
_version_ | 1804182484288536576 |
---|---|
adam_txt | |
any_adam_object | |
any_adam_object_boolean | |
building | Verbundindex |
bvnumber | BV047302585 |
collection | ZDB-2-PRO |
ctrlnum | (ZDB-2-PRO)978-1-0716-0323-9 (OCoLC)1256420033 (DE-599)BVBBV047302585 |
dewey-full | 611.01816 599.935 |
dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
dewey-ones | 611 - Human anatomy, cytology, histology 599 - Mammalia |
dewey-raw | 611.01816 599.935 |
dewey-search | 611.01816 599.935 |
dewey-sort | 3611.01816 |
dewey-tens | 610 - Medicine and health 590 - Animals |
discipline | Biologie Medizin |
discipline_str_mv | Biologie Medizin |
doi_str_mv | 10.1007/978-1-0716-0323-9 |
edition | 1st ed. 2020 |
format | Electronic eBook |
fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>04219nmm a2200457zcb4500</leader><controlfield tag="001">BV047302585</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">210528s2020 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781071603239</subfield><subfield code="9">978-1-07-160323-9</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-0716-0323-9</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-0716-0323-9</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1256420033</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV047302585</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">611.01816</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">599.935</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">DNA Electrophoresis</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Katsuhiro Hanada</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1st ed. 2020</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer US</subfield><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield><subfield code="v">2119</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Introduction and Perspectives of DNA Electrophoresis -- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers -- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage -- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast -- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures -- Alkali Comet Assay in Genotoxicity Tests -- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay -- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis -- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells -- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria -- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE) -- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast -- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes -- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System -- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells -- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation -- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP) -- Post-Labeling/PAGE Method for Detection of DNA Adducts</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biomedical engineering</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hanada, Katsuhiro</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781071603222</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781071603246</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781071603253</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-0716-0323-9</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-032705725</subfield></datafield><datafield tag="347" ind1=" " ind2=" "><subfield code="a">text file</subfield><subfield code="b">PDF</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-0716-0323-9</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-0716-0323-9</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
id | DE-604.BV047302585 |
illustrated | Not Illustrated |
index_date | 2024-07-03T17:23:47Z |
indexdate | 2024-07-10T09:08:17Z |
institution | BVB |
isbn | 9781071603239 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032705725 |
oclc_num | 1256420033 |
open_access_boolean | |
owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
psigel | ZDB-2-PRO |
publishDate | 2020 |
publishDateSearch | 2020 |
publishDateSort | 2020 |
publisher | Springer US |
record_format | marc |
series2 | Methods in Molecular Biology |
spelling | DNA Electrophoresis Methods and Protocols edited by Katsuhiro Hanada 1st ed. 2020 New York, NY Springer US 2020 txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2119 Introduction and Perspectives of DNA Electrophoresis -- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers -- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage -- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast -- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures -- Alkali Comet Assay in Genotoxicity Tests -- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay -- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis -- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells -- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria -- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE) -- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast -- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes -- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System -- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells -- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation -- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP) -- Post-Labeling/PAGE Method for Detection of DNA Adducts This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field Human genetics Biomedical engineering Hanada, Katsuhiro Sonstige oth Erscheint auch als Druck-Ausgabe 9781071603222 Erscheint auch als Druck-Ausgabe 9781071603246 Erscheint auch als Druck-Ausgabe 9781071603253 https://doi.org/10.1007/978-1-0716-0323-9 Verlag URL des Erstveröffentlichers Volltext text file PDF |
spellingShingle | DNA Electrophoresis Methods and Protocols Human genetics Biomedical engineering |
title | DNA Electrophoresis Methods and Protocols |
title_auth | DNA Electrophoresis Methods and Protocols |
title_exact_search | DNA Electrophoresis Methods and Protocols |
title_exact_search_txtP | DNA Electrophoresis Methods and Protocols |
title_full | DNA Electrophoresis Methods and Protocols edited by Katsuhiro Hanada |
title_fullStr | DNA Electrophoresis Methods and Protocols edited by Katsuhiro Hanada |
title_full_unstemmed | DNA Electrophoresis Methods and Protocols edited by Katsuhiro Hanada |
title_short | DNA Electrophoresis |
title_sort | dna electrophoresis methods and protocols |
title_sub | Methods and Protocols |
topic | Human genetics Biomedical engineering |
topic_facet | Human genetics Biomedical engineering |
url | https://doi.org/10.1007/978-1-0716-0323-9 |
work_keys_str_mv | AT hanadakatsuhiro dnaelectrophoresismethodsandprotocols |