MERS Coronavirus: Methods and Protocols
This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-vira...
Gespeichert in:
| Format: | Elektronisch E-Book |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2020
|
| Ausgabe: | 1st ed. 2020 |
| Schriftenreihe: | Methods in Molecular Biology
2099 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Beschreibung: | Studying Evolutionary Adaptation of MERS-CoV -- Evaluating MERS-CoV Entry Pathways -- Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing -- Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein -- Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV -- Deducing the Crystal Structure of MERS-CoV Helicase -- Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans -- Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR -- Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay -- Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera -- Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA -- Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome -- Development of a Mouse Adapted MERS Coronavirus -- Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection -- Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection -- Histopathologic Evaluation and Scoring of Viral Lung Infection. |
| ISBN: | 9781071602119 |
| DOI: | 10.1007/978-1-0716-0211-9 |
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| spelling | MERS Coronavirus Methods and Protocols edited by Rahul Vijay 1st ed. 2020 New York, NY Springer US 2020 txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2099 Studying Evolutionary Adaptation of MERS-CoV -- Evaluating MERS-CoV Entry Pathways -- Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing -- Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein -- Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV -- Deducing the Crystal Structure of MERS-CoV Helicase -- Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans -- Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR -- Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay -- Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera -- Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA -- Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome -- Development of a Mouse Adapted MERS Coronavirus -- Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection -- Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection -- Histopathologic Evaluation and Scoring of Viral Lung Infection. This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field Immunology Virology (DE-588)4143413-4 Aufsatzsammlung gnd-content Vijay, Rahul Sonstige oth Erscheint auch als Druck-Ausgabe 9781071602102 Erscheint auch als Druck-Ausgabe 9781071602126 Erscheint auch als Druck-Ausgabe 9781071602133 https://doi.org/10.1007/978-1-0716-0211-9 Verlag URL des Erstveröffentlichers Volltext text file PDF |
| spellingShingle | MERS Coronavirus Methods and Protocols Immunology Virology |
| subject_GND | (DE-588)4143413-4 |
| title | MERS Coronavirus Methods and Protocols |
| title_auth | MERS Coronavirus Methods and Protocols |
| title_exact_search | MERS Coronavirus Methods and Protocols |
| title_exact_search_txtP | MERS Coronavirus Methods and Protocols |
| title_full | MERS Coronavirus Methods and Protocols edited by Rahul Vijay |
| title_fullStr | MERS Coronavirus Methods and Protocols edited by Rahul Vijay |
| title_full_unstemmed | MERS Coronavirus Methods and Protocols edited by Rahul Vijay |
| title_short | MERS Coronavirus |
| title_sort | mers coronavirus methods and protocols |
| title_sub | Methods and Protocols |
| topic | Immunology Virology |
| topic_facet | Immunology Virology Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-0716-0211-9 |
| work_keys_str_mv | AT vijayrahul merscoronavirusmethodsandprotocols |