This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; amb...

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Format:	Elektronisch E-Book
Sprache:	English
Veröffentlicht:	New York, NY Springer New York 2020
Ausgabe:	1st ed. 2020
Schriftenreihe:	Methods in Molecular Biology 2064
Schlagworte:	Cell biology Metabolism Stoffwechsel Methode Einzelzellanalyse Aufsatzsammlung
Online-Zugang:	UBR01 TUM01 Volltext
Zusammenfassung:	This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field.
Beschreibung:	Single Cell Metabolomics by Mass Spectrometry -- dentification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry -- Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry -- Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis -- Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells -- Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics -- Laser Capture Microdissection - Liquid Vortex Capture - Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells -- Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging -- A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population -- Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry -- Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry -- Compact Quantum Dots for Quantitative Cytology -- Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry -- Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry -- Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics -- Ten Major Future Challenges in Single Cell Metabolomics
ISBN:	9781493998319
DOI:	10.1007/978-1-4939-9831-9