Structure and function of membrane proteins:

This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Weitere Verfasser: Schmidt-Krey, Ingeborg (HerausgeberIn), Gumbart, James C. (HerausgeberIn)
Format: Buch
Sprache:English
Veröffentlicht: New York, NY Humana Press [2021]
Schriftenreihe:Methods in molecular biology 2302
Schlagworte:
Zusammenfassung:This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles
Beschreibung:1. Expression and Purification of Human Mitochondrial Intramembrane Protease PARL; Elena Arutyunova, Laine Lysyk, Melissa Morrison, Cory Brooks, and M. Joanne Lemieux; ; 2. Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies; Kerry M. Strickland, Kasahun Neselu, Arshay J. Grant, Carolann L. Espy, Nael A. McCarty, and Ingeborg Schmidt-Krey; ; 3. Biochemical Characterization of GPCR–G Protein Complex Formation; Filip Pamula and Ching-Ju Tsai; ; 4. Electrophysiological Approaches for the Study of Ion Channel Function; Guiying Cui, Kirsten A. Cottrill, and Nael A. McCarty; ; 5. Isothermal Titration Calorimetry of Membrane Proteins; Han N. Vu, Alan J. Situ, and Tobias S. Ulmer; ; 6. Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level; Kanokporn Chattrakun, Katherine G. Schaefer, Lucas S.
- Chandler, Brendan P. Marsh, and Gavin M. King; ; 7. Structure Determination of Membrane Proteins Using X-Ray Crystallography; Evan Billings, Karl Lundquist, Claire Overly, Karthik Srinivasan, and Nicholas Noinaj; ; 8. Studying Membrane Protein Structures by MicroED; Michael W. Martynowycz and Tamir Gonen; ; 9. Single-Particle Cryo-EM of Membrane Proteins; Dovile Januliene and Arne Moeller; ; 10. Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy; Mary D. Hernando, Joseph O. Primeau, and Howard S. Young; ; 11. NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel; Robert Silvers and Matthew Eddy; ; 12. Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering; Yuqi Wu, Kevin L. Weiss, and Raquel L. Lieberman; ; 13.
- Preparing Membrane Proteins for Simulation Using CHARMM-GUI; Yupeng Li, Jinchan Liu, and James C. Gumbart; ; 14. Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide; William Glass, Jonathan W. Essex, Franca Fraternali, James Gebbie-Rayet, Irene Marzuoli, Marley L. Samways, Philip C. Biggin, and Syma Khalid; ; 15. Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins; Yandong Huang, Jack A. Henderson, and Jana Shen; ; 16. Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions; Dylan Ogden and Mahmoud Moradi; ; 17. Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations; Wesley M. Botello-Smith and Yun Lyna Luo; ; 18.
- Large Scale Molecular Dynamics Simulations of Cellular Compartments; Eric Wilson, John Vant, Jacob Layton, Ryan Boyd, Hyungro Lee, Matteo Turilli, Benjamín Hernández, Sean Wilkinson, Shantenu Jha, Chitrak Gupta, Daipayan Sarkar, and Abhishek Singharoy
Beschreibung:xii, 358 Seiten Illustrationen, Diagramme 885 grams
ISBN:9781071613931

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