Structural proteomics: high-throughput methods
This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2021]
|
| Ausgabe: | third edition |
| Schriftenreihe: | Methods in molecular biology
2305 |
| Schlagworte: | |
| Zusammenfassung: | This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.Chapter 9 is available open access under a CC BY 4.0 license |
| Beschreibung: | This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.Chapter 9 is available open access under a CC BY 4.0 license Part I: Structural Bioinformatics; ; 1. The Protein Data Bank Archive; Sameer Velankar, Stephen K. Burley, Genji Kurisu, Jeffery C. Hoch, and John L. Markley; ; 2. Computational Methods for the Elucidation of Protein Structure and Interactions; Nicholas S. Edmunds and Liam J. McGuffin; ; 3. Methods for Molecular Modeling of Protein Complexes; Tejashree Rajaram Kanitkar, Neeladri Sen, Sanjana Nair, Neelesh Soni, Kaustubh Amritkar, Yogendra Ramtirtha, and M.S. Madhusudhan; ; Part II: Protein Production; ; 4. High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus; Ester Behiels and Jonathan Elegheert; ; 5. Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies; Ganna O. Krasnoselska, Maud Dumoux, Nadisha Gamage, Harish Cheruvara, James Birch, Andrew Quigley, and Raymond J. - Owens; ; 6. Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells; Maren Schubert, Manfred Nimtz, Federico Bertoglio, Stefan Schmelz, Peer Lukat, and Joop van den Heuvel; ; 7. SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering; Hannah Crocker, Barbara Gorda, Martin Pelosse, Deepak Balaji Thimiri Govinda Raj, and Imre Berger; ; 8. Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification; Sylvain Geny, Simon Pichard, Arnaud Poterszman, and Jean-Paul Concordet; ; 9. Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems; Marlitt Stech, Nathanaël Rakotoarinoro, Tamara Teichmann, Anne Zemella, Lena Thoring, and Stefan Kubick; ; Part III: Structure Determination; ; 10. - Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins; Yanzhang Luo, Shengqi Xiang, Alessandra Lucini Paioni, Agnes Adler, Peter Jan Hooikaas, Jijumon A.S., Carsten Janke3, Anna Akhmanova, and Marc Baldus; ; 11. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources; Pierre Aller and Allen M. Orville; ; 12. From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example; Christoph A. Diebolder, Rebecca S. Dillard, and Ludovic Renault; ; 13. Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods; Carlos Oscar S. - Sorzano, Amaya Jiménez-Moreno, David Maluenda, Erney Ramírez-Aportela, Marta Martínez, Ana Cuervo, Robert Melero, Jose Javier Conesa, Ruben Sánchez-García, David Strelak, Jiri Filipovic, Estrella Fernández-Giménez, Federico de Isidro-Gómez, David Herreros, Pablo Conesa, Laura del Caño, Yunior Fonseca, Jorge Jiménez de la Morena, Jose Ramon Macías, Patricia Losana, Roberto Marabini, and Jose-Maria Carazo; ; 14. Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection; Ottilie von Loeffelholz and Bruno P. Klaholz; ; 15. Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology; Jana Moravcová, Radka Dopitová, Matyáš Pinkas, and Jirí Novácek; ; 16. Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED; Emma Danelius and Tamir Gonen |
| Beschreibung: | xii, 344 Seiten Illustrationen, Diagramme 867 grams |
| ISBN: | 9781071614051 |
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| 490 | 1 | |a Methods in molecular biology |v 2305 | |
| 500 | |a This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.Chapter 9 is available open access under a CC BY 4.0 license | ||
| 500 | |a Part I: Structural Bioinformatics; ; 1. The Protein Data Bank Archive; Sameer Velankar, Stephen K. Burley, Genji Kurisu, Jeffery C. Hoch, and John L. Markley; ; 2. Computational Methods for the Elucidation of Protein Structure and Interactions; Nicholas S. Edmunds and Liam J. McGuffin; ; 3. Methods for Molecular Modeling of Protein Complexes; Tejashree Rajaram Kanitkar, Neeladri Sen, Sanjana Nair, Neelesh Soni, Kaustubh Amritkar, Yogendra Ramtirtha, and M.S. Madhusudhan; ; Part II: Protein Production; ; 4. High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus; Ester Behiels and Jonathan Elegheert; ; 5. Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies; Ganna O. Krasnoselska, Maud Dumoux, Nadisha Gamage, Harish Cheruvara, James Birch, Andrew Quigley, and Raymond J. | ||
| 500 | |a - Owens; ; 6. Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells; Maren Schubert, Manfred Nimtz, Federico Bertoglio, Stefan Schmelz, Peer Lukat, and Joop van den Heuvel; ; 7. SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering; Hannah Crocker, Barbara Gorda, Martin Pelosse, Deepak Balaji Thimiri Govinda Raj, and Imre Berger; ; 8. Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification; Sylvain Geny, Simon Pichard, Arnaud Poterszman, and Jean-Paul Concordet; ; 9. Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems; Marlitt Stech, Nathanaël Rakotoarinoro, Tamara Teichmann, Anne Zemella, Lena Thoring, and Stefan Kubick; ; Part III: Structure Determination; ; 10. | ||
| 500 | |a - Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins; Yanzhang Luo, Shengqi Xiang, Alessandra Lucini Paioni, Agnes Adler, Peter Jan Hooikaas, Jijumon A.S., Carsten Janke3, Anna Akhmanova, and Marc Baldus; ; 11. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources; Pierre Aller and Allen M. Orville; ; 12. From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example; Christoph A. Diebolder, Rebecca S. Dillard, and Ludovic Renault; ; 13. Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods; Carlos Oscar S. | ||
| 500 | |a - Sorzano, Amaya Jiménez-Moreno, David Maluenda, Erney Ramírez-Aportela, Marta Martínez, Ana Cuervo, Robert Melero, Jose Javier Conesa, Ruben Sánchez-García, David Strelak, Jiri Filipovic, Estrella Fernández-Giménez, Federico de Isidro-Gómez, David Herreros, Pablo Conesa, Laura del Caño, Yunior Fonseca, Jorge Jiménez de la Morena, Jose Ramon Macías, Patricia Losana, Roberto Marabini, and Jose-Maria Carazo; ; 14. Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection; Ottilie von Loeffelholz and Bruno P. Klaholz; ; 15. Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology; Jana Moravcová, Radka Dopitová, Matyáš Pinkas, and Jirí Novácek; ; 16. Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED; Emma Danelius and Tamir Gonen | ||
| 520 | |a This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.Chapter 9 is available open access under a CC BY 4.0 license | ||
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Datensatz im Suchindex
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| id | DE-604.BV047300649 |
| illustrated | Illustrated |
| index_date | 2024-07-03T17:23:21Z |
| indexdate | 2024-07-10T09:08:14Z |
| institution | BVB |
| isbn | 9781071614051 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032703817 |
| oclc_num | 1260147036 |
| open_access_boolean | |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | xii, 344 Seiten Illustrationen, Diagramme 867 grams |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Structural proteomics high-throughput methods edited by Raymond J. Owens (The Rosalind Franklin Institute, Harwell Science Campus, Didcot, UK) third edition New York, NY Humana Press [2021] xii, 344 Seiten Illustrationen, Diagramme 867 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2305 This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.Chapter 9 is available open access under a CC BY 4.0 license Part I: Structural Bioinformatics; ; 1. The Protein Data Bank Archive; Sameer Velankar, Stephen K. Burley, Genji Kurisu, Jeffery C. Hoch, and John L. Markley; ; 2. Computational Methods for the Elucidation of Protein Structure and Interactions; Nicholas S. Edmunds and Liam J. McGuffin; ; 3. Methods for Molecular Modeling of Protein Complexes; Tejashree Rajaram Kanitkar, Neeladri Sen, Sanjana Nair, Neelesh Soni, Kaustubh Amritkar, Yogendra Ramtirtha, and M.S. Madhusudhan; ; Part II: Protein Production; ; 4. High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus; Ester Behiels and Jonathan Elegheert; ; 5. Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies; Ganna O. Krasnoselska, Maud Dumoux, Nadisha Gamage, Harish Cheruvara, James Birch, Andrew Quigley, and Raymond J. - Owens; ; 6. Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells; Maren Schubert, Manfred Nimtz, Federico Bertoglio, Stefan Schmelz, Peer Lukat, and Joop van den Heuvel; ; 7. SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering; Hannah Crocker, Barbara Gorda, Martin Pelosse, Deepak Balaji Thimiri Govinda Raj, and Imre Berger; ; 8. Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification; Sylvain Geny, Simon Pichard, Arnaud Poterszman, and Jean-Paul Concordet; ; 9. Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems; Marlitt Stech, Nathanaël Rakotoarinoro, Tamara Teichmann, Anne Zemella, Lena Thoring, and Stefan Kubick; ; Part III: Structure Determination; ; 10. - Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins; Yanzhang Luo, Shengqi Xiang, Alessandra Lucini Paioni, Agnes Adler, Peter Jan Hooikaas, Jijumon A.S., Carsten Janke3, Anna Akhmanova, and Marc Baldus; ; 11. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources; Pierre Aller and Allen M. Orville; ; 12. From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example; Christoph A. Diebolder, Rebecca S. Dillard, and Ludovic Renault; ; 13. Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods; Carlos Oscar S. - Sorzano, Amaya Jiménez-Moreno, David Maluenda, Erney Ramírez-Aportela, Marta Martínez, Ana Cuervo, Robert Melero, Jose Javier Conesa, Ruben Sánchez-García, David Strelak, Jiri Filipovic, Estrella Fernández-Giménez, Federico de Isidro-Gómez, David Herreros, Pablo Conesa, Laura del Caño, Yunior Fonseca, Jorge Jiménez de la Morena, Jose Ramon Macías, Patricia Losana, Roberto Marabini, and Jose-Maria Carazo; ; 14. Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection; Ottilie von Loeffelholz and Bruno P. Klaholz; ; 15. Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology; Jana Moravcová, Radka Dopitová, Matyáš Pinkas, and Jirí Novácek; ; 16. Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED; Emma Danelius and Tamir Gonen bicssc bisacsh Life sciences Chemistry Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Strukturaufklärung (DE-588)4183788-5 gnd rswk-swf Biologie Proteomanalyse (DE-588)4596545-6 s Strukturaufklärung (DE-588)4183788-5 s DE-604 Owens, Raymond J. edt Erscheint auch als Online-Ausgabe 978-1-0716-1406-8 Methods in molecular biology 2305 (DE-604)BV035362695 2305 |
| spellingShingle | Structural proteomics high-throughput methods Methods in molecular biology bicssc bisacsh Life sciences Chemistry Proteomanalyse (DE-588)4596545-6 gnd Strukturaufklärung (DE-588)4183788-5 gnd |
| subject_GND | (DE-588)4596545-6 (DE-588)4183788-5 |
| title | Structural proteomics high-throughput methods |
| title_auth | Structural proteomics high-throughput methods |
| title_exact_search | Structural proteomics high-throughput methods |
| title_exact_search_txtP | Structural proteomics high-throughput methods |
| title_full | Structural proteomics high-throughput methods edited by Raymond J. Owens (The Rosalind Franklin Institute, Harwell Science Campus, Didcot, UK) |
| title_fullStr | Structural proteomics high-throughput methods edited by Raymond J. Owens (The Rosalind Franklin Institute, Harwell Science Campus, Didcot, UK) |
| title_full_unstemmed | Structural proteomics high-throughput methods edited by Raymond J. Owens (The Rosalind Franklin Institute, Harwell Science Campus, Didcot, UK) |
| title_short | Structural proteomics |
| title_sort | structural proteomics high throughput methods |
| title_sub | high-throughput methods |
| topic | bicssc bisacsh Life sciences Chemistry Proteomanalyse (DE-588)4596545-6 gnd Strukturaufklärung (DE-588)4183788-5 gnd |
| topic_facet | bicssc bisacsh Life sciences Chemistry Proteomanalyse Strukturaufklärung |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT owensraymondj structuralproteomicshighthroughputmethods |