Small Non-Coding RNAs: methods and protocols
This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Writte...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
HumanaPress
[2021]
|
| Ausgabe: | Second edition |
| Schriftenreihe: | Methods in molecular biology
2300 |
| Schlagworte: | |
| Zusammenfassung: | This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies |
| Beschreibung: | ; PART I INTRODUCTION TO SMALL NON-CODING RNAS; ; 1 Regulatory Non-coding RNAs: An Overview; Camille Virciglio, Yoann Abel, and Mathieu Rederstorff; ; 2 RNA Precipitation; Valérie Bourguignon-Igel, Yoann Abel, and Mathieu Rederstorff; ; 3 Quantitative and Qualitative Assessment of Small RNA Preparations; Virginie Marchand, Lilia Ayadi, Valérie Bourguignon-Igel, and Yuri Motorin; ; 4 Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection; Charbel Alfeghaly, Christelle Aigueperse, Sylvain Maenner, and Isabelle Behm-Ansmant; ; ; PART II DETECTION OF SMALL NON-CODING RNAS; ; 5 Northern Blot Detection of Tiny RNAs; Jana C. Wiegard, M. Amri C. Schlüter, Olga Y. Burenina, Elena A. Kubareva, Gabriele Klug, Arnold Grünweller, and Roland K. - Hartmann; ; 6 Stem-loop qRT-PCR Based Quantification of miRNAs; Yoann Abel and Mathieu Rederstorff; ; 7 Detection and Labeling of Small Non-coding RNAs by Splinted Ligation; Marlène Vayssières, Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, and Magali Blaud; ; 8 Fluorescence In Situ Hybridization Of Small Non-coding RNAs; Valentin Vautrot, Géraud Heckler, Christelle Aigueperse, and Isabelle Behm-Ansmant; ; ; PART III FUNCTIONAL ANALYSIS OF SMALL NON-CODING RNAS; ; ; 9 Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo; Layea Baldini and Stéphane Labialle; ; 10 Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography; Aymeric Sanchez and Sylvain Maenner; ; 11 Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP); Charbel Alfeghaly, Isabelle Behm-Ansmant, - and Sylvain Maenner; ; 12 Gene Reporter Assays to Study miRNA Function; Yoann Abel and Mathieu Rederstorff; ; 13 CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo; Bin Yi, Kristina Larter, and Yaguang Xi; ; ; PART IV HIGH-THROUGHPUT ANALYSIS OF SMALL NON-CODING RNAS; ; ; 14 Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs; Quentin Thuillier and Isabelle Behm-Ansmant; ; 15 Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma ; Virginie Marchand, Adeline Galvanin, and Yuri Motorin; ; 16 Analysis of the RNA and protein complexome by Grad-seq; Jens Hör and Jörg Vogel; ; 17 µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs; Farah Bouhedda, Roger Cubi, Stéphanie Baudrey and Michael Ryckelynck; ; ; PART V STRUCTURAL APPROACHES; ; ; 18 RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT; Fatima Alghoul, - Gilbert Eriani, and Franck Martin; ; 19 Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs; Marie-Eve Chagot, Marc Quinternet, Xavier Manival, and Isabelle Lebars |
| Beschreibung: | x, 270 seiten Illustrationen, Diagramme 729 grams |
| ISBN: | 9781071613856 |
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| 500 | |a - Hartmann; ; 6 Stem-loop qRT-PCR Based Quantification of miRNAs; Yoann Abel and Mathieu Rederstorff; ; 7 Detection and Labeling of Small Non-coding RNAs by Splinted Ligation; Marlène Vayssières, Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, and Magali Blaud; ; 8 Fluorescence In Situ Hybridization Of Small Non-coding RNAs; Valentin Vautrot, Géraud Heckler, Christelle Aigueperse, and Isabelle Behm-Ansmant; ; ; PART III FUNCTIONAL ANALYSIS OF SMALL NON-CODING RNAS; ; ; 9 Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo; Layea Baldini and Stéphane Labialle; ; 10 Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography; Aymeric Sanchez and Sylvain Maenner; ; 11 Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP); Charbel Alfeghaly, Isabelle Behm-Ansmant, | ||
| 500 | |a - and Sylvain Maenner; ; 12 Gene Reporter Assays to Study miRNA Function; Yoann Abel and Mathieu Rederstorff; ; 13 CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo; Bin Yi, Kristina Larter, and Yaguang Xi; ; ; PART IV HIGH-THROUGHPUT ANALYSIS OF SMALL NON-CODING RNAS; ; ; 14 Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs; Quentin Thuillier and Isabelle Behm-Ansmant; ; 15 Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma ; Virginie Marchand, Adeline Galvanin, and Yuri Motorin; ; 16 Analysis of the RNA and protein complexome by Grad-seq; Jens Hör and Jörg Vogel; ; 17 µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs; Farah Bouhedda, Roger Cubi, Stéphanie Baudrey and Michael Ryckelynck; ; ; PART V STRUCTURAL APPROACHES; ; ; 18 RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT; Fatima Alghoul, | ||
| 500 | |a - Gilbert Eriani, and Franck Martin; ; 19 Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs; Marie-Eve Chagot, Marc Quinternet, Xavier Manival, and Isabelle Lebars | ||
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| index_date | 2024-07-03T17:08:37Z |
| indexdate | 2024-07-10T09:06:55Z |
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| isbn | 9781071613856 |
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| spelling | Small Non-Coding RNAs methods and protocols edited by Mathieur Rederstoff Second edition New York, NY HumanaPress [2021] x, 270 seiten Illustrationen, Diagramme 729 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2300 Springer protocols ; PART I INTRODUCTION TO SMALL NON-CODING RNAS; ; 1 Regulatory Non-coding RNAs: An Overview; Camille Virciglio, Yoann Abel, and Mathieu Rederstorff; ; 2 RNA Precipitation; Valérie Bourguignon-Igel, Yoann Abel, and Mathieu Rederstorff; ; 3 Quantitative and Qualitative Assessment of Small RNA Preparations; Virginie Marchand, Lilia Ayadi, Valérie Bourguignon-Igel, and Yuri Motorin; ; 4 Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection; Charbel Alfeghaly, Christelle Aigueperse, Sylvain Maenner, and Isabelle Behm-Ansmant; ; ; PART II DETECTION OF SMALL NON-CODING RNAS; ; 5 Northern Blot Detection of Tiny RNAs; Jana C. Wiegard, M. Amri C. Schlüter, Olga Y. Burenina, Elena A. Kubareva, Gabriele Klug, Arnold Grünweller, and Roland K. - Hartmann; ; 6 Stem-loop qRT-PCR Based Quantification of miRNAs; Yoann Abel and Mathieu Rederstorff; ; 7 Detection and Labeling of Small Non-coding RNAs by Splinted Ligation; Marlène Vayssières, Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, and Magali Blaud; ; 8 Fluorescence In Situ Hybridization Of Small Non-coding RNAs; Valentin Vautrot, Géraud Heckler, Christelle Aigueperse, and Isabelle Behm-Ansmant; ; ; PART III FUNCTIONAL ANALYSIS OF SMALL NON-CODING RNAS; ; ; 9 Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo; Layea Baldini and Stéphane Labialle; ; 10 Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography; Aymeric Sanchez and Sylvain Maenner; ; 11 Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP); Charbel Alfeghaly, Isabelle Behm-Ansmant, - and Sylvain Maenner; ; 12 Gene Reporter Assays to Study miRNA Function; Yoann Abel and Mathieu Rederstorff; ; 13 CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo; Bin Yi, Kristina Larter, and Yaguang Xi; ; ; PART IV HIGH-THROUGHPUT ANALYSIS OF SMALL NON-CODING RNAS; ; ; 14 Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs; Quentin Thuillier and Isabelle Behm-Ansmant; ; 15 Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma ; Virginie Marchand, Adeline Galvanin, and Yuri Motorin; ; 16 Analysis of the RNA and protein complexome by Grad-seq; Jens Hör and Jörg Vogel; ; 17 µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs; Farah Bouhedda, Roger Cubi, Stéphanie Baudrey and Michael Ryckelynck; ; ; PART V STRUCTURAL APPROACHES; ; ; 18 RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT; Fatima Alghoul, - Gilbert Eriani, and Franck Martin; ; 19 Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs; Marie-Eve Chagot, Marc Quinternet, Xavier Manival, and Isabelle Lebars This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies Genetics Life sciences Non-coding RNA (DE-588)7650051-2 gnd rswk-swf Genetik, Gentechnik (DE-588)4143413-4 Aufsatzsammlung gnd-content Non-coding RNA (DE-588)7650051-2 s DE-604 Rederstorff, Mathieu 1979- (DE-588)1069793280 edt Erscheint auch als Online-Ausgabe 978-1-0716-1386-3 Methods in molecular biology 2300 (DE-604)BV035362695 2300 |
| spellingShingle | Small Non-Coding RNAs methods and protocols Methods in molecular biology Genetics Life sciences Non-coding RNA (DE-588)7650051-2 gnd |
| subject_GND | (DE-588)7650051-2 (DE-588)4143413-4 |
| title | Small Non-Coding RNAs methods and protocols |
| title_auth | Small Non-Coding RNAs methods and protocols |
| title_exact_search | Small Non-Coding RNAs methods and protocols |
| title_exact_search_txtP | Small Non-Coding RNAs methods and protocols |
| title_full | Small Non-Coding RNAs methods and protocols edited by Mathieur Rederstoff |
| title_fullStr | Small Non-Coding RNAs methods and protocols edited by Mathieur Rederstoff |
| title_full_unstemmed | Small Non-Coding RNAs methods and protocols edited by Mathieur Rederstoff |
| title_short | Small Non-Coding RNAs |
| title_sort | small non coding rnas methods and protocols |
| title_sub | methods and protocols |
| topic | Genetics Life sciences Non-coding RNA (DE-588)7650051-2 gnd |
| topic_facet | Genetics Life sciences Non-coding RNA Aufsatzsammlung |
| volume_link | (DE-604)BV035362695 |
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