Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes:
Abstract: Limbal melanocytes, located in the basal epithelial layer of the corneoscleral limbus, represent essential components of the corneal epithelial stem cell niche, but, due to difficulties in their isolation and cultivation, their biological roles and potential for stem cell-based tissue engi...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Erlangen ; Nürnberg
Friedrich-Alexander-Universität Erlangen-Nürnberg
2020
|
| Online-Zugang: | Volltext Volltext Volltext |
| Zusammenfassung: | Abstract: Limbal melanocytes, located in the basal epithelial layer of the corneoscleral limbus, represent essential components of the corneal epithelial stem cell niche, but, due to difficulties in their isolation and cultivation, their biological roles and potential for stem cell-based tissue engineering approaches have not been comprehensively studied. Here, we established a protocol for the efficient isolation and cultivation of pure populations of human limbal melanocytes, which could be expanded at high yield by using recombinant laminin (LN)-511-E8 as culture substrate. Co-cultivation of limbal melanocytes with limbal epithelial stem/progenitor cells on fibrin hydrogels pre-incubated with LN-511-E8 resulted in multilayered stratified epithelial constructs within ten days. By reproducing physiological cell–cell and cell–matrix interactions of the native niche environment, these biomimetic co-culture systems provide a promising experimental model for investigating the functional roles of melanocytes in the limbal stem cell niche and their suitability for developing advanced epithelial grafts for ocular surface surface reconstruction |
| Beschreibung: | 1 Online-Ressource |
| DOI: | 10.1038/s41598-020-68120-0 |
Internformat
MARC
| LEADER | 00000nmm a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV047113070 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 210129s2020 gw |||| o||u| ||||||eng d | ||
| 015 | |a 20,O08 |2 dnb | ||
| 024 | 7 | |a urn:nbn:de:bvb:29-opus4-155847 |2 urn | |
| 035 | |a (OCoLC)1235886488 | ||
| 035 | |a (DE-599)BVBBV047113070 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE-BY | ||
| 049 | |a DE-29 | ||
| 084 | |a 610 |2 sdnb | ||
| 084 | |a 617.7 |2 sdnb | ||
| 100 | 1 | |a Polisetti, Naresh |e Verfasser |0 (DE-588)121424887X |4 aut | |
| 245 | 1 | 0 | |a Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes |c Naresh Polisetti, Andreas Gießl, Shen Li, Lydia Sorokin, Friedrich E. Kruse, Ursula Schlötzer-Schrehardt |
| 264 | 1 | |a Erlangen ; Nürnberg |b Friedrich-Alexander-Universität Erlangen-Nürnberg |c 2020 | |
| 300 | |a 1 Online-Ressource | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 520 | 3 | |a Abstract: Limbal melanocytes, located in the basal epithelial layer of the corneoscleral limbus, represent essential components of the corneal epithelial stem cell niche, but, due to difficulties in their isolation and cultivation, their biological roles and potential for stem cell-based tissue engineering approaches have not been comprehensively studied. Here, we established a protocol for the efficient isolation and cultivation of pure populations of human limbal melanocytes, which could be expanded at high yield by using recombinant laminin (LN)-511-E8 as culture substrate. Co-cultivation of limbal melanocytes with limbal epithelial stem/progenitor cells on fibrin hydrogels pre-incubated with LN-511-E8 resulted in multilayered stratified epithelial constructs within ten days. By reproducing physiological cell–cell and cell–matrix interactions of the native niche environment, these biomimetic co-culture systems provide a promising experimental model for investigating the functional roles of melanocytes in the limbal stem cell niche and their suitability for developing advanced epithelial grafts for ocular surface surface reconstruction | |
| 700 | 1 | |a Gießl, Andreas |d 1972- |e Verfasser |0 (DE-588)12964966X |4 aut | |
| 700 | 1 | |a Li, Shen |e Verfasser |4 aut | |
| 700 | 1 | |a Sorokin, Lydia |e Verfasser |4 aut | |
| 700 | 1 | |a Kruse, Friedrich E. |e Verfasser |4 aut | |
| 700 | 1 | |a Schlötzer-Schrehardt, Ursula |d 1957- |e Verfasser |0 (DE-588)115733119X |4 aut | |
| 787 | 0 | 8 | |i Sonderdruck aus |t Scientific Reports |g Vol. 10 (2020) |o 10.1038/s41598-020-68120-0 |
| 856 | 4 | 0 | |u https://nbn-resolving.org/urn:nbn:de:bvb:29-opus4-155847 |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41598-020-68120-0 |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |q application/pdf |u https://open.fau.de/handle/openfau/15584 |x Verlag |z kostenfrei |3 Volltext |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032519500 | ||
Datensatz im Suchindex
| _version_ | 1804182148764139520 |
|---|---|
| adam_txt | |
| any_adam_object | |
| any_adam_object_boolean | |
| author | Polisetti, Naresh Gießl, Andreas 1972- Li, Shen Sorokin, Lydia Kruse, Friedrich E. Schlötzer-Schrehardt, Ursula 1957- |
| author_GND | (DE-588)121424887X (DE-588)12964966X (DE-588)115733119X |
| author_facet | Polisetti, Naresh Gießl, Andreas 1972- Li, Shen Sorokin, Lydia Kruse, Friedrich E. Schlötzer-Schrehardt, Ursula 1957- |
| author_role | aut aut aut aut aut aut |
| author_sort | Polisetti, Naresh |
| author_variant | n p np a g ag s l sl l s ls f e k fe fek u s s uss |
| building | Verbundindex |
| bvnumber | BV047113070 |
| collection | ebook |
| ctrlnum | (OCoLC)1235886488 (DE-599)BVBBV047113070 |
| discipline | Medizin |
| discipline_str_mv | Medizin |
| doi_str_mv | 10.1038/s41598-020-68120-0 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02952nmm a2200433zc 4500</leader><controlfield tag="001">BV047113070</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">210129s2020 gw |||| o||u| ||||||eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">20,O08</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">urn:nbn:de:bvb:29-opus4-155847</subfield><subfield code="2">urn</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1235886488</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV047113070</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-BY</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">617.7</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Polisetti, Naresh</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)121424887X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes</subfield><subfield code="c">Naresh Polisetti, Andreas Gießl, Shen Li, Lydia Sorokin, Friedrich E. Kruse, Ursula Schlötzer-Schrehardt</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Erlangen ; Nürnberg</subfield><subfield code="b">Friedrich-Alexander-Universität Erlangen-Nürnberg</subfield><subfield code="c">2020</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1="3" ind2=" "><subfield code="a">Abstract: Limbal melanocytes, located in the basal epithelial layer of the corneoscleral limbus, represent essential components of the corneal epithelial stem cell niche, but, due to difficulties in their isolation and cultivation, their biological roles and potential for stem cell-based tissue engineering approaches have not been comprehensively studied. Here, we established a protocol for the efficient isolation and cultivation of pure populations of human limbal melanocytes, which could be expanded at high yield by using recombinant laminin (LN)-511-E8 as culture substrate. Co-cultivation of limbal melanocytes with limbal epithelial stem/progenitor cells on fibrin hydrogels pre-incubated with LN-511-E8 resulted in multilayered stratified epithelial constructs within ten days. By reproducing physiological cell–cell and cell–matrix interactions of the native niche environment, these biomimetic co-culture systems provide a promising experimental model for investigating the functional roles of melanocytes in the limbal stem cell niche and their suitability for developing advanced epithelial grafts for ocular surface surface reconstruction</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gießl, Andreas</subfield><subfield code="d">1972-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)12964966X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Shen</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sorokin, Lydia</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kruse, Friedrich E.</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Schlötzer-Schrehardt, Ursula</subfield><subfield code="d">1957-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)115733119X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="787" ind1="0" ind2="8"><subfield code="i">Sonderdruck aus</subfield><subfield code="t">Scientific Reports</subfield><subfield code="g">Vol. 10 (2020)</subfield><subfield code="o">10.1038/s41598-020-68120-0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:29-opus4-155847</subfield><subfield code="x">Resolving-System</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1038/s41598-020-68120-0</subfield><subfield code="x">Resolving-System</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="q">application/pdf</subfield><subfield code="u">https://open.fau.de/handle/openfau/15584</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-032519500</subfield></datafield></record></collection> |
| id | DE-604.BV047113070 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T16:26:49Z |
| indexdate | 2024-07-10T09:02:57Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032519500 |
| oclc_num | 1235886488 |
| open_access_boolean | 1 |
| owner | DE-29 |
| owner_facet | DE-29 |
| physical | 1 Online-Ressource |
| psigel | ebook |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Friedrich-Alexander-Universität Erlangen-Nürnberg |
| record_format | marc |
| spelling | Polisetti, Naresh Verfasser (DE-588)121424887X aut Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes Naresh Polisetti, Andreas Gießl, Shen Li, Lydia Sorokin, Friedrich E. Kruse, Ursula Schlötzer-Schrehardt Erlangen ; Nürnberg Friedrich-Alexander-Universität Erlangen-Nürnberg 2020 1 Online-Ressource txt rdacontent c rdamedia cr rdacarrier Abstract: Limbal melanocytes, located in the basal epithelial layer of the corneoscleral limbus, represent essential components of the corneal epithelial stem cell niche, but, due to difficulties in their isolation and cultivation, their biological roles and potential for stem cell-based tissue engineering approaches have not been comprehensively studied. Here, we established a protocol for the efficient isolation and cultivation of pure populations of human limbal melanocytes, which could be expanded at high yield by using recombinant laminin (LN)-511-E8 as culture substrate. Co-cultivation of limbal melanocytes with limbal epithelial stem/progenitor cells on fibrin hydrogels pre-incubated with LN-511-E8 resulted in multilayered stratified epithelial constructs within ten days. By reproducing physiological cell–cell and cell–matrix interactions of the native niche environment, these biomimetic co-culture systems provide a promising experimental model for investigating the functional roles of melanocytes in the limbal stem cell niche and their suitability for developing advanced epithelial grafts for ocular surface surface reconstruction Gießl, Andreas 1972- Verfasser (DE-588)12964966X aut Li, Shen Verfasser aut Sorokin, Lydia Verfasser aut Kruse, Friedrich E. Verfasser aut Schlötzer-Schrehardt, Ursula 1957- Verfasser (DE-588)115733119X aut Sonderdruck aus Scientific Reports Vol. 10 (2020) 10.1038/s41598-020-68120-0 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus4-155847 Resolving-System kostenfrei Volltext https://doi.org/10.1038/s41598-020-68120-0 Resolving-System kostenfrei Volltext application/pdf https://open.fau.de/handle/openfau/15584 Verlag kostenfrei Volltext |
| spellingShingle | Polisetti, Naresh Gießl, Andreas 1972- Li, Shen Sorokin, Lydia Kruse, Friedrich E. Schlötzer-Schrehardt, Ursula 1957- Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes |
| title | Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes |
| title_auth | Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes |
| title_exact_search | Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes |
| title_exact_search_txtP | Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes |
| title_full | Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes Naresh Polisetti, Andreas Gießl, Shen Li, Lydia Sorokin, Friedrich E. Kruse, Ursula Schlötzer-Schrehardt |
| title_fullStr | Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes Naresh Polisetti, Andreas Gießl, Shen Li, Lydia Sorokin, Friedrich E. Kruse, Ursula Schlötzer-Schrehardt |
| title_full_unstemmed | Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes Naresh Polisetti, Andreas Gießl, Shen Li, Lydia Sorokin, Friedrich E. Kruse, Ursula Schlötzer-Schrehardt |
| title_short | Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes |
| title_sort | laminin 511 e8 promotes efficient in vitro expansion of human limbal melanocytes |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:29-opus4-155847 https://doi.org/10.1038/s41598-020-68120-0 https://open.fau.de/handle/openfau/15584 |
| work_keys_str_mv | AT polisettinaresh laminin511e8promotesefficientinvitroexpansionofhumanlimbalmelanocytes AT gießlandreas laminin511e8promotesefficientinvitroexpansionofhumanlimbalmelanocytes AT lishen laminin511e8promotesefficientinvitroexpansionofhumanlimbalmelanocytes AT sorokinlydia laminin511e8promotesefficientinvitroexpansionofhumanlimbalmelanocytes AT krusefriedriche laminin511e8promotesefficientinvitroexpansionofhumanlimbalmelanocytes AT schlotzerschrehardtursula laminin511e8promotesefficientinvitroexpansionofhumanlimbalmelanocytes |