Patch Clamp Electrophysiology: Methods and Protocols
This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex,...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2021
|
| Ausgabe: | 1st edition |
| Schriftenreihe: | Methods in Molecular Biology
2188 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be areference guide for current and future ion channel physiologists. |
| Beschreibung: | An Introduction to Patch Clamp Recording -- Patch Clamp Technology in the 21st Century -- Heterologous Expression of Ion Channels in Mammalian Cell Lines -- Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel -- Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology -- Multi-electrode Arrays -- Utilising Automated Electrophysiological Platforms in Epilepsy Research -- Dynamic Clamp on a Windows PC -- Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing -- Bioluminescence Methodology for Ion Channel Studies -- Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons -- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method -- In Vivo Patch Clamp Studies -- In Vivo Optogenetics with Stimulus Calibration -- Single or Double Patch Clamp Recordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics -- Optogenetics and Optical Tools in Automated Patch Clamping. |
| Beschreibung: | 1 Online-Ressource (X, 333 Seiten) Illustrationen |
| ISBN: | 9781071608180 |
| DOI: | 10.1007/978-1-0716-0818-0 |
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| isbn | 9781071608180 |
| language | English |
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| spelling | Patch Clamp Electrophysiology Methods and Protocols edited by Mark Dallas, Damian Bell 1st edition New York, NY Springer US 2021 1 Online-Ressource (X, 333 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2188 An Introduction to Patch Clamp Recording -- Patch Clamp Technology in the 21st Century -- Heterologous Expression of Ion Channels in Mammalian Cell Lines -- Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel -- Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology -- Multi-electrode Arrays -- Utilising Automated Electrophysiological Platforms in Epilepsy Research -- Dynamic Clamp on a Windows PC -- Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing -- Bioluminescence Methodology for Ion Channel Studies -- Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons -- Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method -- In Vivo Patch Clamp Studies -- In Vivo Optogenetics with Stimulus Calibration -- Single or Double Patch Clamp Recordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics -- Optogenetics and Optical Tools in Automated Patch Clamping. This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be areference guide for current and future ion channel physiologists. Human physiology Neurosciences Dallas, Mark edt Bell, Damian edt Erscheint auch als Druck-Ausgabe 9781071608173 Erscheint auch als Druck-Ausgabe 9781071608197 Erscheint auch als Druck-Ausgabe 9781071608203 https://doi.org/10.1007/978-1-0716-0818-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Patch Clamp Electrophysiology Methods and Protocols Human physiology Neurosciences |
| title | Patch Clamp Electrophysiology Methods and Protocols |
| title_auth | Patch Clamp Electrophysiology Methods and Protocols |
| title_exact_search | Patch Clamp Electrophysiology Methods and Protocols |
| title_exact_search_txtP | Patch Clamp Electrophysiology Methods and Protocols |
| title_full | Patch Clamp Electrophysiology Methods and Protocols edited by Mark Dallas, Damian Bell |
| title_fullStr | Patch Clamp Electrophysiology Methods and Protocols edited by Mark Dallas, Damian Bell |
| title_full_unstemmed | Patch Clamp Electrophysiology Methods and Protocols edited by Mark Dallas, Damian Bell |
| title_short | Patch Clamp Electrophysiology |
| title_sort | patch clamp electrophysiology methods and protocols |
| title_sub | Methods and Protocols |
| topic | Human physiology Neurosciences |
| topic_facet | Human physiology Neurosciences |
| url | https://doi.org/10.1007/978-1-0716-0818-0 |
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