Capturing Chromosome Conformation: Methods and Protocols
This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly su...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2021
|
| Ausgabe: | 1st edition |
| Schriftenreihe: | Methods in Molecular Biology
2157 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization |
| Beschreibung: | Capturing Chromosome Conformation -- The Chromosome Conformation Capture (3C) in Drosophila melanogaster -- 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture -- Analysis, Modeling, and Visualization of Chromosome Conformation Capture Experiments -- Targeted DNase Hi-C -- C-HiC: A High Resolution Method for Unbiased Chromatin Conformation Capture Targeting Small Locus -- Computational Analysis of Hi-C Data -- Profiling Chromatin Landscape at High Resolution and Throughput with 2C-ChIP -- Single-Cell DamID to Capture Contacts between DNA and the Nuclear Lamina in Individual Mammalian Cells -- Formaldehyde-Mediated Snapshot of Nuclear Architecture -- Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHOR Labeling -- Preparing Map of Chromosome Territories Distribution Frequency -- Higher-Order Chromatin Organization Using 3D DNA Fluorescent In Situ Hybridization -- 3D Immuno-DNA Fluorescence In Situ Hybridization (FISH) for Detection of HIV-1 and Cellular Genes in Primary CD4+ T Cells -- Visualization of Nuclear and Cytoplasmic Long Noncoding RNAs at Single-Cell Level by RNA-FISH -- 3D COMBO chrRNA-DNA-Immuno FISH -- An Algorithm for the Analysis of the 3D Spatial Organization of the Genome |
| Beschreibung: | 1 Online-Ressource (XI, 322 Seiten) Illustrationen |
| ISBN: | 9781071606643 |
| DOI: | 10.1007/978-1-0716-0664-3 |
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| isbn | 9781071606643 |
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| spelling | Capturing Chromosome Conformation Methods and Protocols edited by Beatrice Bodega, Chiara Lanzuolo 1st edition New York, NY Springer US 2021 1 Online-Ressource (XI, 322 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2157 Capturing Chromosome Conformation -- The Chromosome Conformation Capture (3C) in Drosophila melanogaster -- 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture -- Analysis, Modeling, and Visualization of Chromosome Conformation Capture Experiments -- Targeted DNase Hi-C -- C-HiC: A High Resolution Method for Unbiased Chromatin Conformation Capture Targeting Small Locus -- Computational Analysis of Hi-C Data -- Profiling Chromatin Landscape at High Resolution and Throughput with 2C-ChIP -- Single-Cell DamID to Capture Contacts between DNA and the Nuclear Lamina in Individual Mammalian Cells -- Formaldehyde-Mediated Snapshot of Nuclear Architecture -- Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHOR Labeling -- Preparing Map of Chromosome Territories Distribution Frequency -- Higher-Order Chromatin Organization Using 3D DNA Fluorescent In Situ Hybridization -- 3D Immuno-DNA Fluorescence In Situ Hybridization (FISH) for Detection of HIV-1 and Cellular Genes in Primary CD4+ T Cells -- Visualization of Nuclear and Cytoplasmic Long Noncoding RNAs at Single-Cell Level by RNA-FISH -- 3D COMBO chrRNA-DNA-Immuno FISH -- An Algorithm for the Analysis of the 3D Spatial Organization of the Genome This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization Biology-Technique Cell biology Genetics Bodega, Beatrice edt Lanzuolo, Chiara edt Erscheint auch als Druck-Ausgabe 9781071606636 Erscheint auch als Druck-Ausgabe 9781071606650 Erscheint auch als Druck-Ausgabe 9781071606667 https://doi.org/10.1007/978-1-0716-0664-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Capturing Chromosome Conformation Methods and Protocols Biology-Technique Cell biology Genetics |
| title | Capturing Chromosome Conformation Methods and Protocols |
| title_auth | Capturing Chromosome Conformation Methods and Protocols |
| title_exact_search | Capturing Chromosome Conformation Methods and Protocols |
| title_exact_search_txtP | Capturing Chromosome Conformation Methods and Protocols |
| title_full | Capturing Chromosome Conformation Methods and Protocols edited by Beatrice Bodega, Chiara Lanzuolo |
| title_fullStr | Capturing Chromosome Conformation Methods and Protocols edited by Beatrice Bodega, Chiara Lanzuolo |
| title_full_unstemmed | Capturing Chromosome Conformation Methods and Protocols edited by Beatrice Bodega, Chiara Lanzuolo |
| title_short | Capturing Chromosome Conformation |
| title_sort | capturing chromosome conformation methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biology-Technique Cell biology Genetics |
| topic_facet | Biology-Technique Cell biology Genetics |
| url | https://doi.org/10.1007/978-1-0716-0664-3 |
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