Testicular Germ Cell Tumors: Methods and Protocols
This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2021
|
| Ausgabe: | 1st edition |
| Schriftenreihe: | Methods in Molecular Biology
2195 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field. |
| Beschreibung: | Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research -- Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men -- Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection -- Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors -- Germ Cell Tumor Cell Culture Techniques -- Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops -- Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9 -- Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines -- Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines -- Production and Analysis of Human Primordial Germ Cell-Like Cells -- A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors -- Targeted Methylation Analyses: From Bisulfite Treatment to Quantification -- Integrated Analysis of Germ Cell Tumors -- Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors -- A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring -- Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors -- Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors |
| Beschreibung: | 1 Online-Ressource (XII, 278 Seiten) Illustrationen |
| ISBN: | 9781071608609 |
| DOI: | 10.1007/978-1-0716-0860-9 |
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| 500 | |a Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research -- Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men -- Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection -- Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors -- Germ Cell Tumor Cell Culture Techniques -- Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops -- Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9 -- Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines -- Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines -- Production and Analysis of Human Primordial Germ Cell-Like Cells -- A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors -- Targeted Methylation Analyses: From Bisulfite Treatment to Quantification -- Integrated Analysis of Germ Cell Tumors -- Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors -- A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring -- Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors -- Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors | ||
| 520 | |a This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field. | ||
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| spelling | Testicular Germ Cell Tumors Methods and Protocols edited by Aditya Bagrodia, James F. Amatruda 1st edition New York, NY Springer US 2021 1 Online-Ressource (XII, 278 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2195 Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research -- Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men -- Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection -- Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors -- Germ Cell Tumor Cell Culture Techniques -- Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops -- Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9 -- Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines -- Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines -- Production and Analysis of Human Primordial Germ Cell-Like Cells -- A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors -- Targeted Methylation Analyses: From Bisulfite Treatment to Quantification -- Integrated Analysis of Germ Cell Tumors -- Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors -- A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring -- Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors -- Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field. Developmental biology Cancer research Cell biology Bagrodia, Aditya edt Amatruda, James F. edt Erscheint auch als Druck-Ausgabe 9781071608593 Erscheint auch als Druck-Ausgabe 9781071608616 Erscheint auch als Druck-Ausgabe 9781071608623 https://doi.org/10.1007/978-1-0716-0860-9 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Testicular Germ Cell Tumors Methods and Protocols Developmental biology Cancer research Cell biology |
| title | Testicular Germ Cell Tumors Methods and Protocols |
| title_auth | Testicular Germ Cell Tumors Methods and Protocols |
| title_exact_search | Testicular Germ Cell Tumors Methods and Protocols |
| title_exact_search_txtP | Testicular Germ Cell Tumors Methods and Protocols |
| title_full | Testicular Germ Cell Tumors Methods and Protocols edited by Aditya Bagrodia, James F. Amatruda |
| title_fullStr | Testicular Germ Cell Tumors Methods and Protocols edited by Aditya Bagrodia, James F. Amatruda |
| title_full_unstemmed | Testicular Germ Cell Tumors Methods and Protocols edited by Aditya Bagrodia, James F. Amatruda |
| title_short | Testicular Germ Cell Tumors |
| title_sort | testicular germ cell tumors methods and protocols |
| title_sub | Methods and Protocols |
| topic | Developmental biology Cancer research Cell biology |
| topic_facet | Developmental biology Cancer research Cell biology |
| url | https://doi.org/10.1007/978-1-0716-0860-9 |
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