Reactive Oxygen Species: Methods and Protocols
This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems. Written in the highly success...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2021
|
| Ausgabe: | 1st edition |
| Schriftenreihe: | Methods in Molecular Biology
2202 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Beschreibung: | Introduction to in silico Modeling to Study ROS Dynamics -- Assessment of ROS Production in Mitochondria of Live Cells -- Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy -- A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo Culture -- Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana -- Characterization of oxidative lipidomics and autophagy induction in Chlamydomonas reinhardtii under abiotic stress -- Fluorescence Detection of Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the Diauxic Shift -- Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa -- Measurement of Reactive Oxygen Species In Semen Samples Using Chemiluminescence -- Methods to Unravel Pathways of Reactive Oxygen Species in the Photodynamic Inactivation of Bacteria -- Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence Microscopy Approach -- EPR Spectroscopy as a Method for ROS Quantification in Skin -- Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones -- Singlet Oxygen Quantum Yield Determination Using Chemical Acceptors -- A General Method to Establish the Relative Efficiency of Different Sonosensitizers to Generate ROS for SDT -- Lipid Peroxidation Assay using BODIPY-phenylbutadiene Probes:A Methodological Overview -- Use of Fluorescent Probes to Detect ROS in Photodynamic Therapy. |
| Beschreibung: | 1 Online-Ressource (XI, 233 Seiten) Illustrationen |
| ISBN: | 9781071608968 |
| DOI: | 10.1007/978-1-0716-0896-8 |
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| id | DE-604.BV047051670 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T16:08:17Z |
| indexdate | 2024-07-10T09:01:13Z |
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| isbn | 9781071608968 |
| language | English |
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| spelling | Reactive Oxygen Species Methods and Protocols edited by Jesús Espada 1st edition New York, NY Springer US 2021 1 Online-Ressource (XI, 233 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2202 Introduction to in silico Modeling to Study ROS Dynamics -- Assessment of ROS Production in Mitochondria of Live Cells -- Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy -- A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo Culture -- Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana -- Characterization of oxidative lipidomics and autophagy induction in Chlamydomonas reinhardtii under abiotic stress -- Fluorescence Detection of Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the Diauxic Shift -- Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa -- Measurement of Reactive Oxygen Species In Semen Samples Using Chemiluminescence -- Methods to Unravel Pathways of Reactive Oxygen Species in the Photodynamic Inactivation of Bacteria -- Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence Microscopy Approach -- EPR Spectroscopy as a Method for ROS Quantification in Skin -- Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones -- Singlet Oxygen Quantum Yield Determination Using Chemical Acceptors -- A General Method to Establish the Relative Efficiency of Different Sonosensitizers to Generate ROS for SDT -- Lipid Peroxidation Assay using BODIPY-phenylbutadiene Probes:A Methodological Overview -- Use of Fluorescent Probes to Detect ROS in Photodynamic Therapy. This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field Biochemistry Espada, Jesús edt Erscheint auch als Druck-Ausgabe 9781071608951 Erscheint auch als Druck-Ausgabe 9781071608975 Erscheint auch als Druck-Ausgabe 9781071608982 https://doi.org/10.1007/978-1-0716-0896-8 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Reactive Oxygen Species Methods and Protocols Biochemistry |
| title | Reactive Oxygen Species Methods and Protocols |
| title_auth | Reactive Oxygen Species Methods and Protocols |
| title_exact_search | Reactive Oxygen Species Methods and Protocols |
| title_exact_search_txtP | Reactive Oxygen Species Methods and Protocols |
| title_full | Reactive Oxygen Species Methods and Protocols edited by Jesús Espada |
| title_fullStr | Reactive Oxygen Species Methods and Protocols edited by Jesús Espada |
| title_full_unstemmed | Reactive Oxygen Species Methods and Protocols edited by Jesús Espada |
| title_short | Reactive Oxygen Species |
| title_sort | reactive oxygen species methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biochemistry |
| topic_facet | Biochemistry |
| url | https://doi.org/10.1007/978-1-0716-0896-8 |
| work_keys_str_mv | AT espadajesus reactiveoxygenspeciesmethodsandprotocols |