Cardiac Regeneration: Methods and Protocols
This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury model...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2021
|
| Ausgabe: | 1st edition |
| Schriftenreihe: | Methods in Molecular Biology
2158 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them. The chapter "Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com |
| Beschreibung: | Myocardial Infarction Techniques in Adult Mice -- Apical Resection and Cryoinjury in Neonatal Mouse Heart -- Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice -- Myocardial Infarction in Pigs -- Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish -- Cardiac Resection Injury in Zebrafish -- A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish -- Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration -- Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes -- Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes -- Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells -- In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells -- Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs -- Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells -- Isolation and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts -- Ex Vivo Techniques to Study Heart Regeneration in Zebrafish -- Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters -- In Vivo Clonal Analysis of Cardiomyocytes -- High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry -- AAV Gene Transfer to the Heart -- In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy -- Generation and Manipulation of Exosomes -- Epigenetic Assays in Purified Cardiomyocyte Nuclei -- Genetic Lineage Tracing of Non-Cardiomyocytes in Mice -- Experimental Hypoxia as a Model for Cardiac Regeneration in Mice |
| Beschreibung: | 1 Online-Ressource (XIII, 346 Seiten) Illustrationen |
| ISBN: | 9781071606681 |
| DOI: | 10.1007/978-1-0716-0668-1 |
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| 520 | |a This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them. The chapter "Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com | ||
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Datensatz im Suchindex
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| dewey-full | 571.538 612.028 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 571 - Physiology & related subjects 612 - Human physiology |
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| dewey-tens | 570 - Biology 610 - Medicine and health |
| discipline | Biologie Medizin |
| discipline_str_mv | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-0716-0668-1 |
| edition | 1st edition |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| index_date | 2024-07-03T16:08:16Z |
| indexdate | 2024-07-10T09:01:13Z |
| institution | BVB |
| isbn | 9781071606681 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032458994 |
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| spelling | Cardiac Regeneration Methods and Protocols edited by Kenneth D. Poss, Bernhard Kühn 1st edition New York, NY Springer US 2021 1 Online-Ressource (XIII, 346 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2158 Myocardial Infarction Techniques in Adult Mice -- Apical Resection and Cryoinjury in Neonatal Mouse Heart -- Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice -- Myocardial Infarction in Pigs -- Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish -- Cardiac Resection Injury in Zebrafish -- A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish -- Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration -- Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes -- Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes -- Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells -- In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells -- Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs -- Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells -- Isolation and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts -- Ex Vivo Techniques to Study Heart Regeneration in Zebrafish -- Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters -- In Vivo Clonal Analysis of Cardiomyocytes -- High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry -- AAV Gene Transfer to the Heart -- In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy -- Generation and Manipulation of Exosomes -- Epigenetic Assays in Purified Cardiomyocyte Nuclei -- Genetic Lineage Tracing of Non-Cardiomyocytes in Mice -- Experimental Hypoxia as a Model for Cardiac Regeneration in Mice This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them. The chapter "Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com Regenerative medicine Tissue engineering Physiology Biology-Technique Poss, Kenneth D. edt Kühn, Bernhard edt Erscheint auch als Druck-Ausgabe 9781071606674 Erscheint auch als Druck-Ausgabe 9781071606698 Erscheint auch als Druck-Ausgabe 9781071606704 https://doi.org/10.1007/978-1-0716-0668-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Cardiac Regeneration Methods and Protocols Regenerative medicine Tissue engineering Physiology Biology-Technique |
| title | Cardiac Regeneration Methods and Protocols |
| title_auth | Cardiac Regeneration Methods and Protocols |
| title_exact_search | Cardiac Regeneration Methods and Protocols |
| title_exact_search_txtP | Cardiac Regeneration Methods and Protocols |
| title_full | Cardiac Regeneration Methods and Protocols edited by Kenneth D. Poss, Bernhard Kühn |
| title_fullStr | Cardiac Regeneration Methods and Protocols edited by Kenneth D. Poss, Bernhard Kühn |
| title_full_unstemmed | Cardiac Regeneration Methods and Protocols edited by Kenneth D. Poss, Bernhard Kühn |
| title_short | Cardiac Regeneration |
| title_sort | cardiac regeneration methods and protocols |
| title_sub | Methods and Protocols |
| topic | Regenerative medicine Tissue engineering Physiology Biology-Technique |
| topic_facet | Regenerative medicine Tissue engineering Physiology Biology-Technique |
| url | https://doi.org/10.1007/978-1-0716-0668-1 |
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