Exocytosis and Endocytosis: Methods and Protocols
This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells. Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins i...
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| Weitere Verfasser: | , , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer US
2021
|
| Ausgabe: | 1st edition |
| Schriftenreihe: | Methods in Molecular Biology
2233 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells. Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of exosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and clearly written, Exocytosis and Endocytosis: Methods and Protocols is a valuable resource for researchers in the fields of cell biology, neurology, immunology, oncology, and those interested in studying protein trafficking and signal regulation |
| Beschreibung: | Stoichiometry of Receptors at the Plasma Membrane during Their Endocytosis using Total Internal Reflection Fluorescent (TIRF) Microscopy Live Imaging and Single Molecule Tracking -- Measuring Endocytosis During Proliferative Cell Quiescence -- Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution In Adrenal Chromaffin Cells -- Quantitative Methods to Study Endocytosis and Retrograde Transport of Cargo Proteins -- High-content Drug Discovery Screening of Endocytosis Pathways -- Methods for Monitoring Endocytosis in Astrocytes -- Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent Dextrans -- Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion -- High-throughput Screening for Insulin Secretion Modulators -- Different Approaches to Record Human Sperm Exocytosis -- Bovine Chromaffin Cells: Culture and Fluorescent Assay for Secretion -- Measurement of Exocytosis in Genetically Manipulated Mast Cells -- Super-Resolution Microscopy and Particle Tracking Approaches for the Study of Vesicular Trafficking in Primary Neutrophils -- An Approach to Monitor Exocytosis in White Adipocytes -- Amperometry in Single Cells and Tissue -- Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging In Mouse Adrenal Chromaffin Cells -- RUSH-synchronized Secretion to Measure Local Exocytosis -- Combining Single Molecule Super-Resolution Imaging Techniques to Unravel the Nanoscale Organization of the Presynapse -- Induction of Ca2+ Dependent Exocytotic Processes by Laser Ablation of Endothelial Cells -- Transmission Electron Microscopy and Tomography on Plasma Membrane Sheets to Study Secretory Docking -- Spatial and Temporal Aspects 0f Exocytosis Studied on the Isolated Plasma Membranes |
| Beschreibung: | 1 Online-Ressource (XII, 329 Seiten) Illustrationen |
| ISBN: | 9781071610442 |
| DOI: | 10.1007/978-1-0716-1044-2 |
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| isbn | 9781071610442 |
| language | English |
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| spelling | Exocytosis and Endocytosis Methods and Protocols edited by Florence Niedergang, Nicolas Vitale, Stéphane Gasman 1st edition New York, NY Springer US 2021 1 Online-Ressource (XII, 329 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 2233 Stoichiometry of Receptors at the Plasma Membrane during Their Endocytosis using Total Internal Reflection Fluorescent (TIRF) Microscopy Live Imaging and Single Molecule Tracking -- Measuring Endocytosis During Proliferative Cell Quiescence -- Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution In Adrenal Chromaffin Cells -- Quantitative Methods to Study Endocytosis and Retrograde Transport of Cargo Proteins -- High-content Drug Discovery Screening of Endocytosis Pathways -- Methods for Monitoring Endocytosis in Astrocytes -- Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent Dextrans -- Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion -- High-throughput Screening for Insulin Secretion Modulators -- Different Approaches to Record Human Sperm Exocytosis -- Bovine Chromaffin Cells: Culture and Fluorescent Assay for Secretion -- Measurement of Exocytosis in Genetically Manipulated Mast Cells -- Super-Resolution Microscopy and Particle Tracking Approaches for the Study of Vesicular Trafficking in Primary Neutrophils -- An Approach to Monitor Exocytosis in White Adipocytes -- Amperometry in Single Cells and Tissue -- Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging In Mouse Adrenal Chromaffin Cells -- RUSH-synchronized Secretion to Measure Local Exocytosis -- Combining Single Molecule Super-Resolution Imaging Techniques to Unravel the Nanoscale Organization of the Presynapse -- Induction of Ca2+ Dependent Exocytotic Processes by Laser Ablation of Endothelial Cells -- Transmission Electron Microscopy and Tomography on Plasma Membrane Sheets to Study Secretory Docking -- Spatial and Temporal Aspects 0f Exocytosis Studied on the Isolated Plasma Membranes This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells. Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of exosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and clearly written, Exocytosis and Endocytosis: Methods and Protocols is a valuable resource for researchers in the fields of cell biology, neurology, immunology, oncology, and those interested in studying protein trafficking and signal regulation Cell biology Life sciences Endocytose (DE-588)4152168-7 gnd rswk-swf Exocytose (DE-588)4276172-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Endocytose (DE-588)4152168-7 s Exocytose (DE-588)4276172-4 s DE-604 Niedergang, Florence edt Vitale, Nicolas edt Gasman, Stéphane edt Erscheint auch als Druck-Ausgabe 9781071610435 Erscheint auch als Druck-Ausgabe 9781071610459 Erscheint auch als Druck-Ausgabe 9781071610466 https://doi.org/10.1007/978-1-0716-1044-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Exocytosis and Endocytosis Methods and Protocols Cell biology Life sciences Endocytose (DE-588)4152168-7 gnd Exocytose (DE-588)4276172-4 gnd |
| subject_GND | (DE-588)4152168-7 (DE-588)4276172-4 (DE-588)4143413-4 |
| title | Exocytosis and Endocytosis Methods and Protocols |
| title_auth | Exocytosis and Endocytosis Methods and Protocols |
| title_exact_search | Exocytosis and Endocytosis Methods and Protocols |
| title_exact_search_txtP | Exocytosis and Endocytosis Methods and Protocols |
| title_full | Exocytosis and Endocytosis Methods and Protocols edited by Florence Niedergang, Nicolas Vitale, Stéphane Gasman |
| title_fullStr | Exocytosis and Endocytosis Methods and Protocols edited by Florence Niedergang, Nicolas Vitale, Stéphane Gasman |
| title_full_unstemmed | Exocytosis and Endocytosis Methods and Protocols edited by Florence Niedergang, Nicolas Vitale, Stéphane Gasman |
| title_short | Exocytosis and Endocytosis |
| title_sort | exocytosis and endocytosis methods and protocols |
| title_sub | Methods and Protocols |
| topic | Cell biology Life sciences Endocytose (DE-588)4152168-7 gnd Exocytose (DE-588)4276172-4 gnd |
| topic_facet | Cell biology Life sciences Endocytose Exocytose Aufsatzsammlung |
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