Kidney biomarkers: clinical aspects and laboratory determination
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Bibliographische Detailangaben
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: Amsterdam Academic Press 2020
Schlagworte:
Online-Zugang:TUM01
Beschreibung:Includes index
Further reading -- Chapter 2: Utility of the ''omics ́́in kidney disease: Methods of analysis, sampling considerations, and technical approa ... -- Introduction -- Statistical methods in analysis and interpretation of biomarkers in kidney disease -- Metrics for prediction performance (diagnostic potential) -- Quantification of prediction performance -- True positive rate and false positive rate -- Receiver-operating characteristic curve -- Summary indices -- Area under the ROC curve (AUC) -- Optimal prediction threshold -- Partial area under the curve
Limitations of statistical metrics used for prediction performance -- Metrics to evaluating incremental diagnostic value -- Incremental value: How to evaluate? -- Improvement in discrimination -- Improvement in reclassification -- Net reclassification improvement -- Categorical net reclassification improvement -- Weighted net reclassification improvement -- Category-free net reclassification improvement -- Integrated discrimination improvement -- Decision and clinical use of analytic measures -- Recommendations for sample collection, storage, and preparation -- Tissue samples -- Biofluids
Nontargeted versus targeted analysis -- Proteomics and metabolomics in nephrology -- Potential impact of proteomics and metabolomics in renal diseases -- Proteomics and the kidney -- The proteomics sample -- Urine -- Kidney tissue and cell culture -- Techniques used in proteome analysis -- Proteomics methods based on mass spectrometry -- First step: Protein separation -- Gel-based proteome analysis -- Gel-free proteome analysis -- Liquid chromatography (LC) -- Capillary electrophoresis -- Second step: Protein identification
Two-dimensional gel electrophoresis coupled with mass spectrometry (2DE-MS) -- Liquid chromatography coupled to mass spectrometry (LC-MS/MS) -- Capillary electrophoresis coupled with mass spectrometry (CE-MS) -- Matrix-assisted laser desorption/ionization-time of flight coupled with mass spectrometry (MALDI-TOF/MS) -- Solid-phase chromatography coupled with mass spectrometry (SELDI-MS) -- Differential protein profiling -- Mass spectrometry -- (a) Peptide mass fingerprinting -- (b) Electrospray ionization -- (c) Peptide sequencing using tandem mass spectrometers
Beschreibung:1 Online-Ressource
ISBN:9780128163740
0128163747

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