Monitoring early signalling events in cells using high-time resolution Phosphoproteomics:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Düren
Shaker Verlag
2020
|
| Ausgabe: | [1. Auflage] |
| Schriftenreihe: | Berichte aus der Biochemie
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | VII, 155 Seiten Illustrationen 21 cm, 251 g |
| ISBN: | 9783844074321 3844074325 |
Internformat
MARC
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| 100 | 1 | |a Dell'Aica, Margherita |d 1988- |e Verfasser |0 (DE-588)1214650805 |4 aut | |
| 245 | 1 | 0 | |a Monitoring early signalling events in cells using high-time resolution Phosphoproteomics |c Margherita Dell'Aica |
| 250 | |a [1. Auflage] | ||
| 264 | 1 | |a Düren |b Shaker Verlag |c 2020 | |
| 300 | |a VII, 155 Seiten |b Illustrationen |c 21 cm, 251 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Berichte aus der Biochemie | |
| 502 | |b Dissertation |c Technische Universität Dortmund |d 2019 | ||
| 650 | 0 | 7 | |8 1\p |a Cells |0 (DLC)sh85021678 |0 http://id.loc.gov/authorities/subjects/sh85021678 |2 lcsh |
| 653 | |a Proteomics | ||
| 653 | |a microfluidics | ||
| 653 | |a Mass spectrometry | ||
| 653 | |a EGFR | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 710 | 2 | |a Shaker Verlag |0 (DE-588)1064118135 |4 pbl | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |a Dell'Aica, Margherita, 1988- |t Monitoring early signalling events in cells using high-time resolution Phosphoproteomics |b 1. Auflage |d Düren : Shaker, 2020 |h Online-Ressource, 167 Seiten, 73 Illustrationen |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1211638111/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032365517&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-032365517 | ||
| 883 | 0 | |8 1\p |a maschinell gebildet |c 0,04545 |d 20200813 |q DE-101 | |
Datensatz im Suchindex
| _version_ | 1804181873268621312 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
ABBREVIATIONS
I
LIST
OF
PUBLICATIONS,
PRESENTATIONS
AND
POSTERS
IV
ZUSAMMENFASSUNGVI
SUMMARY
VII
1
INTRODUCTION
1
1.1
TIME
RESOLVED
STUDIES
UPON
CELL
STIMULATION
1
1.1.1
STATE
OF
THE
ART
1
1.1.2
EVOLUTION
AND
CHALLENGES
3
1.2
SIGNALLING
VIA
PTMS
5
1.2.1
PROTEIN
PHOSPHORYLATION
BASED
SIGNALLING
5
1.3
SIGNALLING
BY
RECEPTOR-TYROSINE
KINASES
6
1.3.1
EGFR
SIGNALLING
NETWORK
8
1.4
MASS
SPECTROMETRY-BASED
(PHOSPHO)PROTEOMICS
10
1.4.1
BOTTOM-UP
APPROACHES
IN
PROTEOMICS
10
1.4.2
TITANIUM
DIOXIDE
(TICH)
BASED
PHOSPHOPEPTIDE
ENRICHMENT
11
1.4.3
LIQUID
CHROMATOGRAPHY
13
1.4.4
OFF-LINE
FRACTIONATION:
HILIC
14
1.4.5
REVERSE
PHASE
LIQUID
CHROMATOGRAPHY
15
1.4.6
MASS
SPECTROMETRY
16
1.4.7
PEPTIDE
IDENTIFICATION
IN
MASS
SPECTROMETRY
19
1.4.8
DATA
ACQUISITION
AND
INTERPRETATION
21
1.5
MASS
SPECTROMETRY-BASED
QUANTIFICATION
23
1.5.1
ABSOLUTE
QUANTIFICATION
23
1.5.2
QUANTIFICATION
VIA
TMT
24
1.5.3
QUANTIFICATION
OF
PEPTIDES
VIA
TARGETED
MS
26
1.6
MICROFLUIDICS
28
1.6.1
BASIC
CONCEPTS
IN
MICROFLUIDICS
29
1.6.2
MICROFLUIDICS
FOR
RAPID
CELL
STIMULATION.
ACTIVE
AND
PASSIVE
STRATEGIES
30
1.6.3
PINCHED
FLOW
CELL
SWITCHING
FOR
STIMULATION
STUDIES
32
1.6.4
DIELECTROPHORESIS
FOR
STIMULATION
STUDIES
32
1.6.5
INTEGRATED
STRATEGIES
FOR
STIMULATION
STUDIES
32
1.6.6
MICROMIXING
MICROFLUIDIC
DEVICE
FOR
STIMULATION
STUDIES
33
1.7
MINIATURIZATION
OF
PROTEOMICS
SAMPLE
PREPARATION:
SEPARATION
OF
PROTEINS
VIA
FREE
FLOW
ELECTROPHORESIS
35
1.7.1
ISOELECTRIC
FOCUSING
(IEF)
FOR
PROTEIN
SEPARATION
36
1.7.2
ZONE
ELECTROPHORESIS
(ZE)
FOR
PROTEIN
SEPARATION
37
1.7.3
FREE-FLOW
ELECTROPHORESIS
(FFE)
SEPARATION
ON
A
MICROFLUIDICS
DEVICE
...............................
37
2
AIM
OF
THE
WORK
38
3
MATERIALS
AND
METHODS
39
3.1
MATERIALS
.....................................................................................................................................
39
3.2
CELL
PREPARATION
FOR
STIMULATION
ASSAYS......................................................................................
43
3.2.1
MAMMALIAN
CELL
CULTURE
.......................................................................................................
43
3.2.2
CELL
DETACHMENT
TECHNIQUES
FOR
ADHERENT
CELL
LINES
............................................................
43
3.2.3
STABLE
PROTEIN
OVEREXPRESSION
.............................................................................................
44
3.2.4
MANUAL
CELL
STIMULATION
.......................................................................................................
44
3.3
MICROFLUIDICS
FABRICATION
.............................................................................................................
45
3.3.1
PINCHED
FLOW
SYSTEM
-
1
ST
GENERATION
TEMPORAL
CELL
STIMULATION
STRATEGY
..........................
46
3.3.2
MICROMIXER
MICROFLUIDICS
DEVICE
(M
2
D)
AND
CELL
STIMULATION
ASSAY
-
2
ND
GENERATION
TEMPORAL
CELL
STIMULATION
STRATEGY
....................................................................................................
46
3.3.3
FREE
FLOW
ELECTROPHORESIS
FOR
PROTEIN
AND
PEPTIDE
SEPARATION
..........................................
49
3.4
SAMPLE
PREPARATION
BOTTOM-UP
..................................................................................................
51
3.4.1
CELL
LYSIS,
PROTEIN
CONCENTRATION
MEASUREMENT
AND
CARBAMIDOMETHYLATION
.....................
51
3.4.2
1-STEP
SAMPLE
PREPARATION
WORKFLOWS
.................................................................................
52
3.4.3
2-STEPS
SAMPLE
PREPARATION
WORKFLOW
FOR
TEMPORAL
PHOSPHOPROTEOMICS
.........................
53
3.4.4
SAMPLE
PREPARATION
FOR
FFE
SAMPLES
...................................................................................
53
3.4.5
EVALUATION
OF
DIGESTION
EFFICIENCY
AND
SAMPLE
NORMALIZATION
............................................
54
3.4.6
ITRAQ
LABELLING
OF
CELLS
FOR
TEMPORAL
PHOSPHOPROTEOMICS
ANALYSIS
OF
HE
LA
CELLS
..............
54
3.4.7
TMT
LABELLING
OF
HEK293
EGFR
CELLS
FOR
TEMPORAL
PHOSPHOPROTEOMICS
ANALYSIS
...................
55
3.4.8
TMT
LABELLING
OF
A431
CELLS
FOR
TEMPORAL
PHOSPHOPROTEOMICS
ANALYSIS............................
58
3.4.9
DESALTING
OF
PEPTIDES
VIA
SOLID-PHASE
EXTRACTION
.................................................................
59
3.4.10
PHOSPHOPEPTIDE
ENRICHMENT,
TWO
STEPS
ENRICHMENT
..........................................................
60
3.4.11
PHOSPHOPEPTIDE
ENRICHMENT
ON
A
96
DEEP-WELL
PLATE,
ONE-STEP
ENRICHMENT
...................
62
3.4.12
OFF-LINE
FRACTIONATION
OF
PHOSPHOPEPTIDES
USING
HILIC
.......................................................
63
3.4.13
NANO-LC-MS/MS...................................................................................................................
64
3.4.14
TARGETED
MS
WITH
STABLE
ISOTOPE
LABELLED
(SIL)
REFERENCE
PEPTIDES
...................................
66
3.4.15
TARGETED
MS
FOR
TEMPORAL
PHOSPHOPROTEOMICS
IN
PLATELETS
..............................................
70
3.5
DATABASE
SEARCHES
AND
DATA
ANALYSIS
..........................................................................................
73
3.5.1
PROTEOME
DISCOVERER
SEARCHES............................................................................................
73
3.5.2
DATA
ANALYSIS
FOR
TMT
BASED
QUANTIFICATION
........................................................................
74
3.5.3
CLASSIFICATION
OF
PHOSPHOPEPTIDES
.......................................................................................
77
4
RESULTS
AND
DISCUSSION
81
4.1
THE
IMPORTANCE
OF
HIGH
TEMPORAL
RESOLUTION
..............................................................................
81
4.1.1
GLOBAL
DYNAMIC
RESPONSES
OF
THE
EGFR
PATHWAY
..............................................................
81
4.1.2
HIGH
TEMPORAL
RESOLVED
STUDIES
IN
PLATELETS
.......................................................................
86
4.2
STABLE
EGFR
OVEREXPRESSING
HEK293
CELLS
..................................................................................
89
4.3
EARLY
STAGE
MICROFLUIDICS
DESIGNS
...............................................................................................
92
4.3.1
PINCHED
FLOW
SYSTEM
-
1
ST
GENERATION
TEMPORAL
CELL
STIMULATION
STRATEGY
.........................
93
4.3.2
MICROMIXER-MICROFLUIDICS
DEVICE
-
2
ND
GENERATION
TEMPORAL
CELL
STIMULATION
STRATEGY
.....
95
4.3.3
IMPROVING
SAMPLE
PREPARATION
AFTER
STIMULATION
ON
A
MICROFLUIDICS
DEVICE
......................
97
4.3.4
EVALUATING
THE
SUITABILITY
OF
M
2
D
FOR
CELL
STIMULATION
ASSAYS
............................................
100
4.3.5
USING
M
2
D
FOR
ULTRA-HIGH
TEMPORAL
RESOLUTION
PHOSPHOPROTEOMICS
................................
107
4.4
TARGETED
MS
ASSAYS
FOR
SELECTED
PHOSPHOPEPTIDES
OF
THE
EGFR
PATHWAY
..............................
ILL
4.4.1
CALIBRATION
CURVES
FOR
SIL
PEPTIDES
....................................................................................
112
4.4.2
DETERMINING
THE
EXPRESSION
LEVELS
OF
SELECTED
EGFR
PATHWAY
COMPONENTS
...................
114
4.4.3
EVALUATION
OF
PHOSPHOPEPTIDE
ENRICHMENT
PARALLELIZATION
USING
96
WELL
PLATES
..............
119
4.4.4
TARGETED
ANALYSIS
OF
EARLY
EGFR
SIGNALLING
IN
HEK293
EGFR
.................................................
122
4.4.5
TEMPORAL
PROFILES
OF
EARLY
EGFR
SIGNALLING
IN
HEK293
EGFR
.................................................
125
4.5
SUITABILITY
OF
CELL
STIMULATION
ON
THE
M
2
D
FOR
OTHER
CELL
LINES
..................................................
128
4.5.1
ALTERNATIVE
METHODS
FOR
CELL
DETACHMENT
..........................................................................
128
4.5.2
A431
CELL
STIMULATION
WITH
EGF
USING
MICROFLUIDICS
............................................................
129
4.6
MICRO-FFE
FOR
PROTEIN
AND
PEPTIDES
MIXTURE
..........................................................................
132
4.6.1
MICRO-FFE-ZE
FOR
PROTEIN
MASS
SPECTROMETRY
...................................................................
132
4.6.2
PFF-IEF
FOR
PROTEIN
SEPARATION
...........................................................................................
135
5
CONCLUSION
AND
PROSPECTIVE
138
5.1
SUMMARY
AND
FINAL
ANALYSIS
.......................................................................................................
138
5.2
FUTURE
PROSPECTIVE
..................................................................................................................
143
REFERENCES
145
ACKNOWLEDGEMENTS
155
|
| adam_txt |
TABLE
OF
CONTENTS
ABBREVIATIONS
I
LIST
OF
PUBLICATIONS,
PRESENTATIONS
AND
POSTERS
IV
ZUSAMMENFASSUNGVI
SUMMARY
VII
1
INTRODUCTION
1
1.1
TIME
RESOLVED
STUDIES
UPON
CELL
STIMULATION
1
1.1.1
STATE
OF
THE
ART
1
1.1.2
EVOLUTION
AND
CHALLENGES
3
1.2
SIGNALLING
VIA
PTMS
5
1.2.1
PROTEIN
PHOSPHORYLATION
BASED
SIGNALLING
5
1.3
SIGNALLING
BY
RECEPTOR-TYROSINE
KINASES
6
1.3.1
EGFR
SIGNALLING
NETWORK
8
1.4
MASS
SPECTROMETRY-BASED
(PHOSPHO)PROTEOMICS
10
1.4.1
BOTTOM-UP
APPROACHES
IN
PROTEOMICS
10
1.4.2
TITANIUM
DIOXIDE
(TICH)
BASED
PHOSPHOPEPTIDE
ENRICHMENT
11
1.4.3
LIQUID
CHROMATOGRAPHY
13
1.4.4
OFF-LINE
FRACTIONATION:
HILIC
14
1.4.5
REVERSE
PHASE
LIQUID
CHROMATOGRAPHY
15
1.4.6
MASS
SPECTROMETRY
16
1.4.7
PEPTIDE
IDENTIFICATION
IN
MASS
SPECTROMETRY
19
1.4.8
DATA
ACQUISITION
AND
INTERPRETATION
21
1.5
MASS
SPECTROMETRY-BASED
QUANTIFICATION
23
1.5.1
ABSOLUTE
QUANTIFICATION
23
1.5.2
QUANTIFICATION
VIA
TMT
24
1.5.3
QUANTIFICATION
OF
PEPTIDES
VIA
TARGETED
MS
26
1.6
MICROFLUIDICS
28
1.6.1
BASIC
CONCEPTS
IN
MICROFLUIDICS
29
1.6.2
MICROFLUIDICS
FOR
RAPID
CELL
STIMULATION.
ACTIVE
AND
PASSIVE
STRATEGIES
30
1.6.3
PINCHED
FLOW
CELL
SWITCHING
FOR
STIMULATION
STUDIES
32
1.6.4
DIELECTROPHORESIS
FOR
STIMULATION
STUDIES
32
1.6.5
INTEGRATED
STRATEGIES
FOR
STIMULATION
STUDIES
32
1.6.6
MICROMIXING
MICROFLUIDIC
DEVICE
FOR
STIMULATION
STUDIES
33
1.7
MINIATURIZATION
OF
PROTEOMICS
SAMPLE
PREPARATION:
SEPARATION
OF
PROTEINS
VIA
FREE
FLOW
ELECTROPHORESIS
35
1.7.1
ISOELECTRIC
FOCUSING
(IEF)
FOR
PROTEIN
SEPARATION
36
1.7.2
ZONE
ELECTROPHORESIS
(ZE)
FOR
PROTEIN
SEPARATION
37
1.7.3
FREE-FLOW
ELECTROPHORESIS
(FFE)
SEPARATION
ON
A
MICROFLUIDICS
DEVICE
.
37
2
AIM
OF
THE
WORK
38
3
MATERIALS
AND
METHODS
39
3.1
MATERIALS
.
39
3.2
CELL
PREPARATION
FOR
STIMULATION
ASSAYS.
43
3.2.1
MAMMALIAN
CELL
CULTURE
.
43
3.2.2
CELL
DETACHMENT
TECHNIQUES
FOR
ADHERENT
CELL
LINES
.
43
3.2.3
STABLE
PROTEIN
OVEREXPRESSION
.
44
3.2.4
MANUAL
CELL
STIMULATION
.
44
3.3
MICROFLUIDICS
FABRICATION
.
45
3.3.1
PINCHED
FLOW
SYSTEM
-
1
ST
GENERATION
TEMPORAL
CELL
STIMULATION
STRATEGY
.
46
3.3.2
MICROMIXER
MICROFLUIDICS
DEVICE
(M
2
D)
AND
CELL
STIMULATION
ASSAY
-
2
ND
GENERATION
TEMPORAL
CELL
STIMULATION
STRATEGY
.
46
3.3.3
FREE
FLOW
ELECTROPHORESIS
FOR
PROTEIN
AND
PEPTIDE
SEPARATION
.
49
3.4
SAMPLE
PREPARATION
BOTTOM-UP
.
51
3.4.1
CELL
LYSIS,
PROTEIN
CONCENTRATION
MEASUREMENT
AND
CARBAMIDOMETHYLATION
.
51
3.4.2
1-STEP
SAMPLE
PREPARATION
WORKFLOWS
.
52
3.4.3
2-STEPS
SAMPLE
PREPARATION
WORKFLOW
FOR
TEMPORAL
PHOSPHOPROTEOMICS
.
53
3.4.4
SAMPLE
PREPARATION
FOR
FFE
SAMPLES
.
53
3.4.5
EVALUATION
OF
DIGESTION
EFFICIENCY
AND
SAMPLE
NORMALIZATION
.
54
3.4.6
ITRAQ
LABELLING
OF
CELLS
FOR
TEMPORAL
PHOSPHOPROTEOMICS
ANALYSIS
OF
HE
LA
CELLS
.
54
3.4.7
TMT
LABELLING
OF
HEK293
EGFR
CELLS
FOR
TEMPORAL
PHOSPHOPROTEOMICS
ANALYSIS
.
55
3.4.8
TMT
LABELLING
OF
A431
CELLS
FOR
TEMPORAL
PHOSPHOPROTEOMICS
ANALYSIS.
58
3.4.9
DESALTING
OF
PEPTIDES
VIA
SOLID-PHASE
EXTRACTION
.
59
3.4.10
PHOSPHOPEPTIDE
ENRICHMENT,
TWO
STEPS
ENRICHMENT
.
60
3.4.11
PHOSPHOPEPTIDE
ENRICHMENT
ON
A
96
DEEP-WELL
PLATE,
ONE-STEP
ENRICHMENT
.
62
3.4.12
OFF-LINE
FRACTIONATION
OF
PHOSPHOPEPTIDES
USING
HILIC
.
63
3.4.13
NANO-LC-MS/MS.
64
3.4.14
TARGETED
MS
WITH
STABLE
ISOTOPE
LABELLED
(SIL)
REFERENCE
PEPTIDES
.
66
3.4.15
TARGETED
MS
FOR
TEMPORAL
PHOSPHOPROTEOMICS
IN
PLATELETS
.
70
3.5
DATABASE
SEARCHES
AND
DATA
ANALYSIS
.
73
3.5.1
PROTEOME
DISCOVERER
SEARCHES.
73
3.5.2
DATA
ANALYSIS
FOR
TMT
BASED
QUANTIFICATION
.
74
3.5.3
CLASSIFICATION
OF
PHOSPHOPEPTIDES
.
77
4
RESULTS
AND
DISCUSSION
81
4.1
THE
IMPORTANCE
OF
HIGH
TEMPORAL
RESOLUTION
.
81
4.1.1
GLOBAL
DYNAMIC
RESPONSES
OF
THE
EGFR
PATHWAY
.
81
4.1.2
HIGH
TEMPORAL
RESOLVED
STUDIES
IN
PLATELETS
.
86
4.2
STABLE
EGFR
OVEREXPRESSING
HEK293
CELLS
.
89
4.3
EARLY
STAGE
MICROFLUIDICS
DESIGNS
.
92
4.3.1
PINCHED
FLOW
SYSTEM
-
1
ST
GENERATION
TEMPORAL
CELL
STIMULATION
STRATEGY
.
93
4.3.2
MICROMIXER-MICROFLUIDICS
DEVICE
-
2
ND
GENERATION
TEMPORAL
CELL
STIMULATION
STRATEGY
.
95
4.3.3
IMPROVING
SAMPLE
PREPARATION
AFTER
STIMULATION
ON
A
MICROFLUIDICS
DEVICE
.
97
4.3.4
EVALUATING
THE
SUITABILITY
OF
M
2
D
FOR
CELL
STIMULATION
ASSAYS
.
100
4.3.5
USING
M
2
D
FOR
ULTRA-HIGH
TEMPORAL
RESOLUTION
PHOSPHOPROTEOMICS
.
107
4.4
TARGETED
MS
ASSAYS
FOR
SELECTED
PHOSPHOPEPTIDES
OF
THE
EGFR
PATHWAY
.
ILL
4.4.1
CALIBRATION
CURVES
FOR
SIL
PEPTIDES
.
112
4.4.2
DETERMINING
THE
EXPRESSION
LEVELS
OF
SELECTED
EGFR
PATHWAY
COMPONENTS
.
114
4.4.3
EVALUATION
OF
PHOSPHOPEPTIDE
ENRICHMENT
PARALLELIZATION
USING
96
WELL
PLATES
.
119
4.4.4
TARGETED
ANALYSIS
OF
EARLY
EGFR
SIGNALLING
IN
HEK293
EGFR
.
122
4.4.5
TEMPORAL
PROFILES
OF
EARLY
EGFR
SIGNALLING
IN
HEK293
EGFR
.
125
4.5
SUITABILITY
OF
CELL
STIMULATION
ON
THE
M
2
D
FOR
OTHER
CELL
LINES
.
128
4.5.1
ALTERNATIVE
METHODS
FOR
CELL
DETACHMENT
.
128
4.5.2
A431
CELL
STIMULATION
WITH
EGF
USING
MICROFLUIDICS
.
129
4.6
MICRO-FFE
FOR
PROTEIN
AND
PEPTIDES
MIXTURE
.
132
4.6.1
MICRO-FFE-ZE
FOR
PROTEIN
MASS
SPECTROMETRY
.
132
4.6.2
PFF-IEF
FOR
PROTEIN
SEPARATION
.
135
5
CONCLUSION
AND
PROSPECTIVE
138
5.1
SUMMARY
AND
FINAL
ANALYSIS
.
138
5.2
FUTURE
PROSPECTIVE
.
143
REFERENCES
145
ACKNOWLEDGEMENTS
155 |
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| author | Dell'Aica, Margherita 1988- |
| author_GND | (DE-588)1214650805 |
| author_facet | Dell'Aica, Margherita 1988- |
| author_role | aut |
| author_sort | Dell'Aica, Margherita 1988- |
| author_variant | m d md |
| building | Verbundindex |
| bvnumber | BV046957095 |
| ctrlnum | (OCoLC)1220892944 (DE-599)DNB1211638111 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| edition | [1. Auflage] |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV046957095 |
| illustrated | Illustrated |
| index_date | 2024-07-03T15:42:52Z |
| indexdate | 2024-07-10T08:58:34Z |
| institution | BVB |
| institution_GND | (DE-588)1064118135 |
| isbn | 9783844074321 3844074325 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032365517 |
| oclc_num | 1220892944 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | VII, 155 Seiten Illustrationen 21 cm, 251 g |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Shaker Verlag |
| record_format | marc |
| series2 | Berichte aus der Biochemie |
| spelling | Dell'Aica, Margherita 1988- Verfasser (DE-588)1214650805 aut Monitoring early signalling events in cells using high-time resolution Phosphoproteomics Margherita Dell'Aica [1. Auflage] Düren Shaker Verlag 2020 VII, 155 Seiten Illustrationen 21 cm, 251 g txt rdacontent n rdamedia nc rdacarrier Berichte aus der Biochemie Dissertation Technische Universität Dortmund 2019 1\p Cells (DLC)sh85021678 http://id.loc.gov/authorities/subjects/sh85021678 lcsh Proteomics microfluidics Mass spectrometry EGFR (DE-588)4113937-9 Hochschulschrift gnd-content Shaker Verlag (DE-588)1064118135 pbl Erscheint auch als Online-Ausgabe Dell'Aica, Margherita, 1988- Monitoring early signalling events in cells using high-time resolution Phosphoproteomics 1. Auflage Düren : Shaker, 2020 Online-Ressource, 167 Seiten, 73 Illustrationen B:DE-101 application/pdf https://d-nb.info/1211638111/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032365517&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p maschinell gebildet 0,04545 20200813 DE-101 |
| spellingShingle | Dell'Aica, Margherita 1988- Monitoring early signalling events in cells using high-time resolution Phosphoproteomics 1\p Cells (DLC)sh85021678 http://id.loc.gov/authorities/subjects/sh85021678 lcsh |
| subject_GND | (DLC)sh85021678 http://id.loc.gov/authorities/subjects/sh85021678 (DE-588)4113937-9 |
| title | Monitoring early signalling events in cells using high-time resolution Phosphoproteomics |
| title_auth | Monitoring early signalling events in cells using high-time resolution Phosphoproteomics |
| title_exact_search | Monitoring early signalling events in cells using high-time resolution Phosphoproteomics |
| title_exact_search_txtP | Monitoring early signalling events in cells using high-time resolution Phosphoproteomics |
| title_full | Monitoring early signalling events in cells using high-time resolution Phosphoproteomics Margherita Dell'Aica |
| title_fullStr | Monitoring early signalling events in cells using high-time resolution Phosphoproteomics Margherita Dell'Aica |
| title_full_unstemmed | Monitoring early signalling events in cells using high-time resolution Phosphoproteomics Margherita Dell'Aica |
| title_short | Monitoring early signalling events in cells using high-time resolution Phosphoproteomics |
| title_sort | monitoring early signalling events in cells using high time resolution phosphoproteomics |
| topic | 1\p Cells (DLC)sh85021678 http://id.loc.gov/authorities/subjects/sh85021678 lcsh |
| topic_facet | Cells Hochschulschrift |
| url | https://d-nb.info/1211638111/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032365517&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT dellaicamargherita monitoringearlysignallingeventsincellsusinghightimeresolutionphosphoproteomics AT shakerverlag monitoringearlysignallingeventsincellsusinghightimeresolutionphosphoproteomics |
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