Coronaviruses: methods and protocols
This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with cover...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2020]
|
| Ausgabe: | Second edition |
| Schriftenreihe: | Methods in molecular biology
2203 |
| Schlagworte: | |
| Zusammenfassung: | This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals |
| Beschreibung: | 1. Coronaviruses: An Updated Overview of Their Replication and Pathogenesis; Yuhang Wang, Matthew Grunewald, and Stanley Perlman; ; Part I: Coronavirus Detection, Discovery, and Evolution; ; 2. Discovery of Novel Coronaviruses in Rodents; Theocharis Tsoleridis and Jonathan K. Ball; ; 3. Detection and Discovery of Coronaviruses in Wild Bird Populations; Chantal J. Snoeck and Siamak Zohari; ; 4. Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV); Shuetsu Fukushi; ; 5. Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing; Graham L. Freimanis and Michael S. Oade; ; Part II: Propagation and Titration of Coronaviruses; ; 6. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study; Hui Hu, Kwonil Jung, Scott P. Kenney, and Linda J. Saif; ; 7. - The Preparation of Chicken Kidney Cell Cultures for Virus Propagation; Elena Lokhman, Srijana Rai, and Billy Matthews; ; 8. The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation; Giulia Dowgier and Erica Bickerton; ; 9. Isolation and Propagation of Coronaviruses in Embryonated Eggs; James S. Guy; ; 10. Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures; Mitra Gultom, Laura Laloli, and Ronald Dijkman; ; 11. Quantification of Coronaviruses by Titration In Vitro and Ex Vivo; Giulia Dowgier and Helena J. Maier; ; Part III: Manipulating the Genomes of Coronaviruses; ; 12. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses; Sarah Keep, Paul Britton, and Erica Bickerton; ; 13. - In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline; Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Joerg Jores, and Volker Thiel; ; Part IV: Studying Virus-Host Interactions; ; 14. Proximity Labeling for Identification of Coronavirus-Host Protein Interactions; Philip V’kovski, Silvio Steiner, and Volker Thiel; ; 15. Using Yeast to Identify Coronavirus-Host Protein Interactions; Stuart Weston and Matthew Frieman; ; 16. Determining How Coronaviruses Overcome the Interferon and Innate Immune Response; Christopher M. Coleman; ; 17. Ribopuromycylation in Coronavirus-Infected Cells; Matthew J. Brownsword, Helena J. Maier, and Nicolas Locker; ; Part V: Imaging Coronavirus Infections; ; 18. Visualizing Coronavirus Entry into Cells; Aleksandra Milewska, Katarzyna Owczarek, Artur Szczepanski, and Krzysztof Pyrc; ; 19. - Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope; Nicole Doyle and Philippa C. Hawes |
| Beschreibung: | xi, 278 Seiten Illustrationen 751 grams |
| ISBN: | 9781071608999 |
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| 500 | |a - Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope; Nicole Doyle and Philippa C. Hawes | ||
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| spelling | Coronaviruses methods and protocols edited by Helena J. Maier and Erice Bickteron, the Pirbright Institute, Surrey, UK Second edition New York, NY Humana Press [2020] xi, 278 Seiten Illustrationen 751 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2203 Springer Protocols 1. Coronaviruses: An Updated Overview of Their Replication and Pathogenesis; Yuhang Wang, Matthew Grunewald, and Stanley Perlman; ; Part I: Coronavirus Detection, Discovery, and Evolution; ; 2. Discovery of Novel Coronaviruses in Rodents; Theocharis Tsoleridis and Jonathan K. Ball; ; 3. Detection and Discovery of Coronaviruses in Wild Bird Populations; Chantal J. Snoeck and Siamak Zohari; ; 4. Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV); Shuetsu Fukushi; ; 5. Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing; Graham L. Freimanis and Michael S. Oade; ; Part II: Propagation and Titration of Coronaviruses; ; 6. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study; Hui Hu, Kwonil Jung, Scott P. Kenney, and Linda J. Saif; ; 7. - The Preparation of Chicken Kidney Cell Cultures for Virus Propagation; Elena Lokhman, Srijana Rai, and Billy Matthews; ; 8. The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation; Giulia Dowgier and Erica Bickerton; ; 9. Isolation and Propagation of Coronaviruses in Embryonated Eggs; James S. Guy; ; 10. Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures; Mitra Gultom, Laura Laloli, and Ronald Dijkman; ; 11. Quantification of Coronaviruses by Titration In Vitro and Ex Vivo; Giulia Dowgier and Helena J. Maier; ; Part III: Manipulating the Genomes of Coronaviruses; ; 12. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses; Sarah Keep, Paul Britton, and Erica Bickerton; ; 13. - In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline; Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Joerg Jores, and Volker Thiel; ; Part IV: Studying Virus-Host Interactions; ; 14. Proximity Labeling for Identification of Coronavirus-Host Protein Interactions; Philip V’kovski, Silvio Steiner, and Volker Thiel; ; 15. Using Yeast to Identify Coronavirus-Host Protein Interactions; Stuart Weston and Matthew Frieman; ; 16. Determining How Coronaviruses Overcome the Interferon and Innate Immune Response; Christopher M. Coleman; ; 17. Ribopuromycylation in Coronavirus-Infected Cells; Matthew J. Brownsword, Helena J. Maier, and Nicolas Locker; ; Part V: Imaging Coronavirus Infections; ; 18. Visualizing Coronavirus Entry into Cells; Aleksandra Milewska, Katarzyna Owczarek, Artur Szczepanski, and Krzysztof Pyrc; ; 19. - Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope; Nicole Doyle and Philippa C. Hawes This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals Virology Medicine Coronaviren (DE-588)4276392-7 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Mikrobiologie (DE-588)4143413-4 Aufsatzsammlung gnd-content Coronaviren (DE-588)4276392-7 s Molekularbiologische Methode (DE-588)7650063-9 s DE-604 Maier, Helena J. (DE-588)1218615974 edt Bickerton, Erica (DE-588)1218616105 edt Erscheint auch als Online-Ausgabe 978-1-0716-0900-2 Methods in molecular biology 2203 (DE-604)BV035362695 2203 |
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| subject_GND | (DE-588)4276392-7 (DE-588)7650063-9 (DE-588)4143413-4 |
| title | Coronaviruses methods and protocols |
| title_auth | Coronaviruses methods and protocols |
| title_exact_search | Coronaviruses methods and protocols |
| title_exact_search_txtP | Coronaviruses methods and protocols |
| title_full | Coronaviruses methods and protocols edited by Helena J. Maier and Erice Bickteron, the Pirbright Institute, Surrey, UK |
| title_fullStr | Coronaviruses methods and protocols edited by Helena J. Maier and Erice Bickteron, the Pirbright Institute, Surrey, UK |
| title_full_unstemmed | Coronaviruses methods and protocols edited by Helena J. Maier and Erice Bickteron, the Pirbright Institute, Surrey, UK |
| title_short | Coronaviruses |
| title_sort | coronaviruses methods and protocols |
| title_sub | methods and protocols |
| topic | Virology Medicine Coronaviren (DE-588)4276392-7 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd |
| topic_facet | Virology Medicine Coronaviren Molekularbiologische Methode Aufsatzsammlung |
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