This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various...

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Bibliographische Detailangaben

Weitere Verfasser:	Fahie, Monifa A.V (HerausgeberIn)
Format:	Buch
Sprache:	English
Veröffentlicht:	New York, NY Humana Press [2021]
Schriftenreihe:	Methods in Molecular Biology 2186
Schlagworte:	bicssc bisacsh Proteins  Biophysics Biological physics Biotechnology Life sciences Mikrobiologie
Zusammenfassung:	This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research
Beschreibung:	Part I: Design and Preparation of Biological Based Nanopores; ; 1. Preparation of Fragaceatoxin C (FraC) Nanopores; Natalie Lisa Mutter, Gang Huang, Nieck van der Heide, Florian Leonardus Rudolfus Lucas, Nicole Stéphanie Galenkamp, Giovanni Maglia, and Carsten Wloka; ; 2. Preparation of Cytolysin A (ClyA) Nanopores; Nicole Stéphanie Galenkamp, Veerle Van Meervelt, Natalie Lisa Mutter, Nieck van der Heide, Carsten Wloka, and Giovanni Maglia; ; 3. Building Synthetic Transmembrane Peptide Pores; Kozhinjampara R. Mahendran; ; 4. Design and Assembly of Membrane-Spanning DNA Nanopores; Kerstin Göpfrich and Ulrich F. Keyser; ; Part II: Single Molecule Detection and Analysis of Protein Analytes; ; 5. Determining the Orientation of Porins in Planar Lipid Bilayers; Sandra A Ionescu, Sejeong Lee, and Hagan Bayley; ; 6. Revelation of Function and Inhibition of Wza through Single Channel Studies; Lingbing Kong; ; 7. Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore; Monifa A.V. Fahie, Bib Yang, Christina M. Chisholm, and Min Chen; ; 8. Nanopore Enzymology to Study Protein Kinases and their Inhibition by Small Molecules; Leon Harrington, Leila T. Alexander, Stefan Knapp, and Hagan Bayley; ; 9. A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore; Monifa A.V. Fahie, Bach Pham, Fanjun Li, and Min Chen; ; 10. Oligonucleotide-Directed Protein Threading through a Rigid Nanopore; Garbiñe Celaya and David Rodriguez Larrea; ; 11. Unfolding and Translocation of Proteins through an Alpha-Hemolysin Nanopore by ClpXP; Jeff Nivala, Logan Mulroney, Qing Luan, Robin Abu-Shumays, and Mark Akeson; ; Part III: Computational Analysis of Nanopore Behavior; ; 12. Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel; Alan Perez-Rathke, Monifa A.V. Fahie, Christina M. Chisholm, Min Chen, and Jie Liang; ; 13. Free Energy Minimization for Vesicle Translocation through a Narrow Pore; Hamid R. Shojaei, Ahad Khaleghi Ardabili, and Murrugappan Muthukumar; ; Part IV: Droplet Interface Bilayer System; ; 14. Single Ion-Channel Analysis in Droplet Interface Bilayer; Arash Manafirad; ; 15. Continuous and Rapid Solution Exchange in a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer; En-Hsin Lee; ; 16. Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip; Xin Li and Min Chen
Beschreibung:	x, 231 Seiten Illustrationen, Diagramme 663 grams
ISBN:	9781071608050