Molecular characterisation of circulating metabolites in atrial fibrillation:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Hamburg
2019
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 106, XXVII Blätter Illustrationen, Diagramme 30 cm |
Internformat
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| 100 | 1 | |a Krause, Julia |e Verfasser |0 (DE-588)1198975997 |4 aut | |
| 245 | 1 | 0 | |a Molecular characterisation of circulating metabolites in atrial fibrillation |c vorgelegt von: Julia Krause |
| 264 | 1 | |a Hamburg |c 2019 | |
| 300 | |a 106, XXVII Blätter |b Illustrationen, Diagramme |c 30 cm | ||
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Datensatz im Suchindex
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|---|---|
| adam_text | TABLE
OF
CONTENT
TABLE
OF
CONTENT
I
LIST
OF
FIGURES
IV
LIST
OF
TABLES
VII
1
INTRODUCTION
1
1.1
ATRIAL
FIBRILLATION
1
1.1.1
AF
AND
CARDIAC
METABOLISM
2
1.1.2
RISK
PREDICTION
FOR
AF
3
1.2
ATRIAL
CHARACTERISTICS
AND
IN
VITRO
MODELS
5
1.2.1
COMPARISON
OF
ATRIAL
AND
VENTRICULAR
CARDIOMYOCYTES
5
1.2.2
IN
VITRO
MODELS:
CARDIAC
TISSUE
ENGINEERING
7
1.3
AIM
OF
THE
STUDY
10
2
MATERIALS
11
2.1
ANTIBODIES
11
2.2
BUFFER
AND
SOLUTIONS
11
2.3
CELL
CULTURE
MEDIA
12
2.4
CHEMICALS
14
2.5
CONSUMABLES
16
2.6
MOLECULAR
BIOLOGICAL
KITS
AND
REAGENTS
17
2.7
LABORATORY
EQUIPMENT
17
2.8
PRIMERS
19
2.9
SOFTWARE
20
2.10
TAQMAN
ASSAYS
20
3
METHODS
21
3.1
IDENTIFICATION
OF
UNSATURATED
C18
ACYL-CARNITINES
21
3.1.1
BIOMARCARE
21
3.1.2
AF
CLINICAL
COHORT
STUDY:
AF_CCS
22
3.2
CELL
BIOLOGICAL
METHODS
22
3.2.1
STEM
CELL
CULTURE
22
3.2.2
DIFFERENTIATION
OF
IPSCS
INTO
CARDIOMYOCYTES
23
3.2.3
PREPARATION
OF
IPSC-DERIVED
CARDIOMYOCYTES
FOR
LONG-TERM
STORAGE
24
3.2.4
FLOW
CYTOMETRIC
ANALYSIS
24
3.2.5
ISOLATION
OF
NEONATAL
RAT
CARDIOMYOCYTES
24
3.2.6
CALCIUM
TRANSIENT
MEASUREMENTS
WITH
FURA-2
AM
25
3.2.7
MITOCHONDRIAL
TOXGLO
ASSAY
25
3.2.8
MITOCHONDRIAL
RESPIRATION
27
3.3
EXPERIMENTS
WITH
ENGINEERED
HEART
TISSUE
(EHT)
28
3.3.1
GENERATION
OF
RAT
EHT
28
3.3.2
GENERATION
OF
HUMAN
EHT
28
3.3.3
VIDEO-OPTICAL
CONTRACTION
ANALYSIS
29
3.3.4
TREATMENT
OF
EHT
WITH
ACYL-CARNITINES
29
3.3.5
ELECTRICAL
CAPTURE
AND
AVERAGE
CONTRACTION
PEAKS
30
3.3.6
MUSCARINIC
RECEPTOR
ACTIVATION
30
3.3.7
ACTION
POTENTIAL
MEASUREMENTS
31
3.4
MOLECULAR
ANALYSIS
32
3.4.1
RNA
ANALYSIS
32
3.4.2
PROTEIN
ANALYSIS
35
3.4.3
MEASUREMENTS
OF
METABOLITES
37
3.4.4
GLUCOSE
MEASUREMENTS
37
3.5
STATISTICAL
AND
BIOINFORMATICAL
ANALYSIS
37
3.5.1
BIOMARCARE
37
3.5.2
AF-CCS
38
4
RESULTS
39
4.1
IDENTIFICATION
OF
C18:1
ACYL-CARNITINE
IN
BIOMARCARE
39
4.2
REPLICATION
OF
METABOLITES
IDENTIFIED
IN
BIOMARCARE
IN
AF_CCS
41
4.3
ESTABLISHMENT
OF
THE
RAT
ATRIAL
EHT
MODEL
42
4.4
HUMAN
ATRIAL
EHT
MODEL
54
4.5
EFFECT
OF
CL
8:1
AC
ON
RAT
EHT
57
4.6
EFFECT
OF
C18:1
AC
ON
HUMAN
EHT
61
4.7
EFFECT
OF
018:1
AC
ON
HUMAN
SINGLE
IPSC-DERIVED
CARDIOMYOCYTES
79
4.7.1
CALCIUM
TRANSIENTS
79
4.7.2
MITOCHONDRIAL
METABOLISM
OF
HUMAN
IPSC-DERIVED
CARDIOMYOCYTES
81
4.8
EFFECT
OF
C18:1
ON
ISOLATED
MITOCHONDRIA
OF
MOUSE
CARDIOMYOCYTES
83
4.9
GENE
SET
ENRICHMENT
ANALYSES
(GSEA)
84
5
DISCUSSION
87
5.1
IDENTIFICATION
OF
UNSATURATED
018
ACYL-CARNITINES
(AOS)
87
5.2
FUNCTIONAL
CHARACTERISATION
OF
C1
8:1
AC
89
5.3
SCREENING
FOR
FURTHER
AF-RELATED
METABOLIC
ALTERATIONS
101
II
103
6
CONCLUSION
AND
OUTLOOK
7
SUMMARY
104
8
SUPPLEMENTS
VIII
8.1
TABLES
VIII
9
LIST
OF
ABBREVIATIONS
X
10
REFERENCES
XIII
11
DANKSAGUNG
XXIV
12
LEBENSLAUF
XXVI
EIDESSTATTLICHE
VERSICHERUNG
XXVII
|
| adam_txt |
TABLE
OF
CONTENT
TABLE
OF
CONTENT
I
LIST
OF
FIGURES
IV
LIST
OF
TABLES
VII
1
INTRODUCTION
1
1.1
ATRIAL
FIBRILLATION
1
1.1.1
AF
AND
CARDIAC
METABOLISM
2
1.1.2
RISK
PREDICTION
FOR
AF
3
1.2
ATRIAL
CHARACTERISTICS
AND
IN
VITRO
MODELS
5
1.2.1
COMPARISON
OF
ATRIAL
AND
VENTRICULAR
CARDIOMYOCYTES
5
1.2.2
IN
VITRO
MODELS:
CARDIAC
TISSUE
ENGINEERING
7
1.3
AIM
OF
THE
STUDY
10
2
MATERIALS
11
2.1
ANTIBODIES
11
2.2
BUFFER
AND
SOLUTIONS
11
2.3
CELL
CULTURE
MEDIA
12
2.4
CHEMICALS
14
2.5
CONSUMABLES
16
2.6
MOLECULAR
BIOLOGICAL
KITS
AND
REAGENTS
17
2.7
LABORATORY
EQUIPMENT
17
2.8
PRIMERS
19
2.9
SOFTWARE
20
2.10
TAQMAN
ASSAYS
20
3
METHODS
21
3.1
IDENTIFICATION
OF
UNSATURATED
C18
ACYL-CARNITINES
21
3.1.1
BIOMARCARE
21
3.1.2
AF
CLINICAL
COHORT
STUDY:
AF_CCS
22
3.2
CELL
BIOLOGICAL
METHODS
22
3.2.1
STEM
CELL
CULTURE
22
3.2.2
DIFFERENTIATION
OF
IPSCS
INTO
CARDIOMYOCYTES
23
3.2.3
PREPARATION
OF
IPSC-DERIVED
CARDIOMYOCYTES
FOR
LONG-TERM
STORAGE
24
3.2.4
FLOW
CYTOMETRIC
ANALYSIS
24
3.2.5
ISOLATION
OF
NEONATAL
RAT
CARDIOMYOCYTES
24
3.2.6
CALCIUM
TRANSIENT
MEASUREMENTS
WITH
FURA-2
AM
25
3.2.7
MITOCHONDRIAL
TOXGLO
ASSAY
25
3.2.8
MITOCHONDRIAL
RESPIRATION
27
3.3
EXPERIMENTS
WITH
ENGINEERED
HEART
TISSUE
(EHT)
28
3.3.1
GENERATION
OF
RAT
EHT
28
3.3.2
GENERATION
OF
HUMAN
EHT
28
3.3.3
VIDEO-OPTICAL
CONTRACTION
ANALYSIS
29
3.3.4
TREATMENT
OF
EHT
WITH
ACYL-CARNITINES
29
3.3.5
ELECTRICAL
CAPTURE
AND
AVERAGE
CONTRACTION
PEAKS
30
3.3.6
MUSCARINIC
RECEPTOR
ACTIVATION
30
3.3.7
ACTION
POTENTIAL
MEASUREMENTS
31
3.4
MOLECULAR
ANALYSIS
32
3.4.1
RNA
ANALYSIS
32
3.4.2
PROTEIN
ANALYSIS
35
3.4.3
MEASUREMENTS
OF
METABOLITES
37
3.4.4
GLUCOSE
MEASUREMENTS
37
3.5
STATISTICAL
AND
BIOINFORMATICAL
ANALYSIS
37
3.5.1
BIOMARCARE
37
3.5.2
AF-CCS
38
4
RESULTS
39
4.1
IDENTIFICATION
OF
C18:1
ACYL-CARNITINE
IN
BIOMARCARE
39
4.2
REPLICATION
OF
METABOLITES
IDENTIFIED
IN
BIOMARCARE
IN
AF_CCS
41
4.3
ESTABLISHMENT
OF
THE
RAT
ATRIAL
EHT
MODEL
42
4.4
HUMAN
ATRIAL
EHT
MODEL
54
4.5
EFFECT
OF
CL
8:1
AC
ON
RAT
EHT
57
4.6
EFFECT
OF
C18:1
AC
ON
HUMAN
EHT
61
4.7
EFFECT
OF
018:1
AC
ON
HUMAN
SINGLE
IPSC-DERIVED
CARDIOMYOCYTES
79
4.7.1
CALCIUM
TRANSIENTS
79
4.7.2
MITOCHONDRIAL
METABOLISM
OF
HUMAN
IPSC-DERIVED
CARDIOMYOCYTES
81
4.8
EFFECT
OF
C18:1
ON
ISOLATED
MITOCHONDRIA
OF
MOUSE
CARDIOMYOCYTES
83
4.9
GENE
SET
ENRICHMENT
ANALYSES
(GSEA)
84
5
DISCUSSION
87
5.1
IDENTIFICATION
OF
UNSATURATED
018
ACYL-CARNITINES
(AOS)
87
5.2
FUNCTIONAL
CHARACTERISATION
OF
C1
8:1
AC
89
5.3
SCREENING
FOR
FURTHER
AF-RELATED
METABOLIC
ALTERATIONS
101
II
103
6
CONCLUSION
AND
OUTLOOK
7
SUMMARY
104
8
SUPPLEMENTS
VIII
8.1
TABLES
VIII
9
LIST
OF
ABBREVIATIONS
X
10
REFERENCES
XIII
11
DANKSAGUNG
XXIV
12
LEBENSLAUF
XXVI
EIDESSTATTLICHE
VERSICHERUNG
XXVII |
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| index_date | 2024-07-03T15:01:18Z |
| indexdate | 2024-07-10T08:54:43Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-032227330 |
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| physical | 106, XXVII Blätter Illustrationen, Diagramme 30 cm |
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| spelling | Krause, Julia Verfasser (DE-588)1198975997 aut Molecular characterisation of circulating metabolites in atrial fibrillation vorgelegt von: Julia Krause Hamburg 2019 106, XXVII Blätter Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Hamburg 2019 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1198514000/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032227330&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Krause, Julia Molecular characterisation of circulating metabolites in atrial fibrillation |
| subject_GND | (DE-588)4113937-9 |
| title | Molecular characterisation of circulating metabolites in atrial fibrillation |
| title_auth | Molecular characterisation of circulating metabolites in atrial fibrillation |
| title_exact_search | Molecular characterisation of circulating metabolites in atrial fibrillation |
| title_exact_search_txtP | Molecular characterisation of circulating metabolites in atrial fibrillation |
| title_full | Molecular characterisation of circulating metabolites in atrial fibrillation vorgelegt von: Julia Krause |
| title_fullStr | Molecular characterisation of circulating metabolites in atrial fibrillation vorgelegt von: Julia Krause |
| title_full_unstemmed | Molecular characterisation of circulating metabolites in atrial fibrillation vorgelegt von: Julia Krause |
| title_short | Molecular characterisation of circulating metabolites in atrial fibrillation |
| title_sort | molecular characterisation of circulating metabolites in atrial fibrillation |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1198514000/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032227330&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT krausejulia molecularcharacterisationofcirculatingmetabolitesinatrialfibrillation |
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