Cell-derived matrices: Part B
Gespeichert in:
| Weitere Verfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA ; San Diego, CA
Academic Press
2020
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in cell biology
Volume 157 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xv, 247 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128201749 0128201746 |
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Datensatz im Suchindex
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| adam_text | Contents Contributors............................................................................................................. ix Preface.....................................................................................................................xv SECTION I Tissue deceliularization and _________ characterization methods________________ CHAPTER 1 Deceliularization of porcine heart tissue to obtainextracellular matrix based hydrogels................... 3 Rabeil Sakina, Aida Llucíà-Valldeperas, Ana Henriques Lourenço, Abhishek Harichandan, Sandro Gelsomino, Paul Wieringa, Carlos Mota, Lorenzo Moroni 1. Introduction.................................................................................... 4 2. ECM deceliularization and hydrogel preparation..........................6 3. ECM characterization.................................................................... 9 4. Discussion.....................................................................................14 5. Conclusion.................................................................................... 18 References..........................................................................................18 CHAPTER 2 Method to decellularize the humanchorion membrane.................................................................................23 Laura P. Frazăo, ioana Vieira-de-Castro, Cristina Nogueira-Silva, Nuno M. Neves 1. Introduction.................................................................................. 24 2.
Methodology................................................................................ 25 3. Pitfalls and limitations................................................................. 30 4. Discussion.................................................................................... 30 5. Conclusion....................................................................................32 Acknowledgments............................................................................. 32 References......................................................................................... 32 CHAPTER 3 Protocols for deceliularization ofhuman amniotic membrane.............................................................. 37 Sadjad Khosravimelal, Maryam Momeni, Mahdieh Gholipur, Subhas C. Kundu, Mazaher Gholipourmalekabadi 1. Rationale...................................................................................... 38 2. Materials, equipment and reagents.............................................. 40
VI Contents 3. Decellularization procedures........................................................40 4. Discussion..................................................................................... 44 5. Conclusion.................................................................................... 45 Acknowledgment...............................................................................45 References......................................................................................... 45 CHAPTER 4 Use of supercritical C02 in softtissue decellularization.............................................................. 49 Bengisu Topuz, Gülçin Günal, Selcan Guler, Halil Murat Aydin 1. Introduction...................................................................................50 2. Supercritical processes................................................................. 54 3. Supercritical C02 decellularization methodology: Examples.......................................................................................56 4. Conclusion.................................................................................... 73 Acknowledgments..............................................................................74 References.......................................................................................... 74 CHAPTER 5 Corneal extracellular matrixdecellularization.......... 81 Mark Ahearne 1. Introduction...................................................................................82 2. Decellularization
process..............................................................83 3. Evaluation of comeal extracellular matrix scaffolds...................85 4. Conclusions...................................................................................91 Acknowledgment............................................................................... 91 References.......................................................................................... 91 SECTION II Decellularized tissues for tissue ____________engineering and disease modelling_________ CHAPTER 6 Implementation of a basement membrane invasion assay using mesenterictissue.......................99 Ritobrata Ghose, Alistair J. Rice, Ernesto Cortes, Upamanyu Ghose, Dariusz Lachowski, Armando del Rio Hernandez 1. Introduction................................................................................. 100 2. The mesenteric tissue................................................................. 104 3. The invasion assay......................................................................109 4. Q֊Pi: Quantification of invasion................................................ 112 5. Discussion and evaluation.......................................................... 116 Author contributions and acknowledgment.....................................119 References........................................................................................ 119
Contents CHAPTER 7 Decellularized human bone as a 30 model to study skeletal progenitor cells in a natural environment................................................................... 123 Ana Rita Pereira, Maximilian Rudert, Marietta Herrmann 1. Definition........................................................................................ 124 2. Rationa!........................................................................................... 125 3. Bone decellularization protocols in literature............................. 125 4. Material and equipment................................................................. 128 5. Protocol............................................................................................129 6. Related techniques: Characterization of decellularized bone scaffolds................................................................................. 132 7. Safety considerations......................................................................138 8. Troubleshooting.............................................................................. 139 9. Conclusion.......................................................................................139 References.............................................................................................140 CHAPTER 8 Cell sheet tissue engineering for scaffold-free three-dimensional (3D) tissue reconstruction......... 143 Kyungsook Kim, Sophia Bou-Ghannam, Temo Okano 1. Background..................................................................................... 144 2. Cell sheet tissue
engineering.........................................................147 3. 3D tissue reconstruction................................................................ 151 4. Conclusion.......................................................................................160 Acknowledgments................................................................................161 Competing interests............................................................................. 161 References.............................................................................................161 CHAPTER 9 Decellularized matrices for tumor cell modeling.........................................................................169 Virginia Brancato, Maurizio Ventre, Rui L. Reis, Paolo Antonio Netti 1. Introduction..................................................................................... 171 2. Current methods for extracellular matrix decellularization..............................................................................172 3. Decellularized matrices as support to cellular growth in in vitro cancer models............................................................... 174 4. Materials, equipment and reagents............................................... 175 5. Conclusion.......................................................................................179 6. Safety considerations and standards............................................. 179 7. Pros and cons.................................................................................. 180 8. Troubleshooting and
optimization................................................ 180 vii
viii Contents 9. Summary......................................................................................180 10. General recommendations.......................................................... 181 Acknowledgments..............................................................................181 References.......................................................................................... 181 CHAPTER 10 Tissue-derived decellularized extracellular matrices toward cartilage repair and regeneration....................................................................185 Piyali Das, Yogendra Pratap Singh, Biman B. Mandal, Samit Kumar Nandi 1. Introduction................................................................................. 186 2. Decellularization of tissue-derived ECM matrices.................... 189 3. Evaluation and characterization of decellularized cartilage biomatrix for cartilage regeneration........................................... 199 4. Sterilization of decellularized matrices......................................206 5. Applications of decellularized matrices.....................................208 6. Conclusion.................................................................................. 212 Acknowledgments............................................................................ 213 References........................................................................................ 213 SECTION III Hybrid systems____________________________ CHAPTER 11 The effect of aligned electrospun fibers and macromolecular crowding in tenocyte
culture....... 225 Dimitrios Tsiapalis, Shubhasmin Rana, Meletios Doulgkeroglou, Stephen Kearns, Jack Kelly, Yves Bayon, Dimitrios I. Zeugolis 1. Introduction.................................................................................226 2. Materials and methods................................................................228 3. Results and discussion................................................................ 232 4. Troubleshooting.......................................................................... 240 5. Conclusions.................................................................................241 Acknowledgments............................................................................ 242 Conflict of interest........................................................................... 242 References........................................................................................ 242
|
| adam_txt |
Contents Contributors. ix Preface.xv SECTION I Tissue deceliularization and _ characterization methods_ CHAPTER 1 Deceliularization of porcine heart tissue to obtainextracellular matrix based hydrogels. 3 Rabeil Sakina, Aida Llucíà-Valldeperas, Ana Henriques Lourenço, Abhishek Harichandan, Sandro Gelsomino, Paul Wieringa, Carlos Mota, Lorenzo Moroni 1. Introduction. 4 2. ECM deceliularization and hydrogel preparation.6 3. ECM characterization. 9 4. Discussion.14 5. Conclusion. 18 References.18 CHAPTER 2 Method to decellularize the humanchorion membrane.23 Laura P. Frazăo, ioana Vieira-de-Castro, Cristina Nogueira-Silva, Nuno M. Neves 1. Introduction. 24 2.
Methodology. 25 3. Pitfalls and limitations. 30 4. Discussion. 30 5. Conclusion.32 Acknowledgments. 32 References. 32 CHAPTER 3 Protocols for deceliularization ofhuman amniotic membrane. 37 Sadjad Khosravimelal, Maryam Momeni, Mahdieh Gholipur, Subhas C. Kundu, Mazaher Gholipourmalekabadi 1. Rationale. 38 2. Materials, equipment and reagents. 40
VI Contents 3. Decellularization procedures.40 4. Discussion. 44 5. Conclusion. 45 Acknowledgment.45 References. 45 CHAPTER 4 Use of supercritical C02 in softtissue decellularization. 49 Bengisu Topuz, Gülçin Günal, Selcan Guler, Halil Murat Aydin 1. Introduction.50 2. Supercritical processes. 54 3. Supercritical C02 decellularization methodology: Examples.56 4. Conclusion. 73 Acknowledgments.74 References. 74 CHAPTER 5 Corneal extracellular matrixdecellularization. 81 Mark Ahearne 1. Introduction.82 2. Decellularization
process.83 3. Evaluation of comeal extracellular matrix scaffolds.85 4. Conclusions.91 Acknowledgment. 91 References. 91 SECTION II Decellularized tissues for tissue _engineering and disease modelling_ CHAPTER 6 Implementation of a basement membrane invasion assay using mesenterictissue.99 Ritobrata Ghose, Alistair J. Rice, Ernesto Cortes, Upamanyu Ghose, Dariusz Lachowski, Armando del Rio Hernandez 1. Introduction. 100 2. The mesenteric tissue. 104 3. The invasion assay.109 4. Q֊Pi: Quantification of invasion. 112 5. Discussion and evaluation. 116 Author contributions and acknowledgment.119 References. 119
Contents CHAPTER 7 Decellularized human bone as a 30 model to study skeletal progenitor cells in a natural environment. 123 Ana Rita Pereira, Maximilian Rudert, Marietta Herrmann 1. Definition. 124 2. Rationa!. 125 3. Bone decellularization protocols in literature. 125 4. Material and equipment. 128 5. Protocol.129 6. Related techniques: Characterization of decellularized bone scaffolds. 132 7. Safety considerations.138 8. Troubleshooting. 139 9. Conclusion.139 References.140 CHAPTER 8 Cell sheet tissue engineering for scaffold-free three-dimensional (3D) tissue reconstruction. 143 Kyungsook Kim, Sophia Bou-Ghannam, Temo Okano 1. Background. 144 2. Cell sheet tissue
engineering.147 3. 3D tissue reconstruction. 151 4. Conclusion.160 Acknowledgments.161 Competing interests. 161 References.161 CHAPTER 9 Decellularized matrices for tumor cell modeling.169 Virginia Brancato, Maurizio Ventre, Rui L. Reis, Paolo Antonio Netti 1. Introduction. 171 2. Current methods for extracellular matrix decellularization.172 3. Decellularized matrices as support to cellular growth in in vitro cancer models. 174 4. Materials, equipment and reagents. 175 5. Conclusion.179 6. Safety considerations and standards. 179 7. Pros and cons. 180 8. Troubleshooting and
optimization. 180 vii
viii Contents 9. Summary.180 10. General recommendations. 181 Acknowledgments.181 References. 181 CHAPTER 10 Tissue-derived decellularized extracellular matrices toward cartilage repair and regeneration.185 Piyali Das, Yogendra Pratap Singh, Biman B. Mandal, Samit Kumar Nandi 1. Introduction. 186 2. Decellularization of tissue-derived ECM matrices. 189 3. Evaluation and characterization of decellularized cartilage biomatrix for cartilage regeneration. 199 4. Sterilization of decellularized matrices.206 5. Applications of decellularized matrices.208 6. Conclusion. 212 Acknowledgments. 213 References. 213 SECTION III Hybrid systems_ CHAPTER 11 The effect of aligned electrospun fibers and macromolecular crowding in tenocyte
culture. 225 Dimitrios Tsiapalis, Shubhasmin Rana, Meletios Doulgkeroglou, Stephen Kearns, Jack Kelly, Yves Bayon, Dimitrios I. Zeugolis 1. Introduction.226 2. Materials and methods.228 3. Results and discussion. 232 4. Troubleshooting. 240 5. Conclusions.241 Acknowledgments. 242 Conflict of interest. 242 References. 242 |
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| spelling | Cell-derived matrices Part B edited by David Caballero, Subhas C. Kundu, Rui Luís Reis First edition Cambridge, MA ; San Diego, CA Academic Press 2020 xv, 247 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 157 Methods in cell biology (DE-588)4143413-4 Aufsatzsammlung gnd-content Caballero, David edt Kundu, S. C. (DE-588)1208714082 edt Reis, Rui L. 1967- (DE-588)1194098916 edt (DE-604)BV046796835 B Methods in cell biology Volume 157 (DE-604)BV002534878 157 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032205707&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cell-derived matrices Methods in cell biology |
| subject_GND | (DE-588)4143413-4 |
| title | Cell-derived matrices |
| title_auth | Cell-derived matrices |
| title_exact_search | Cell-derived matrices |
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| title_full | Cell-derived matrices Part B edited by David Caballero, Subhas C. Kundu, Rui Luís Reis |
| title_fullStr | Cell-derived matrices Part B edited by David Caballero, Subhas C. Kundu, Rui Luís Reis |
| title_full_unstemmed | Cell-derived matrices Part B edited by David Caballero, Subhas C. Kundu, Rui Luís Reis |
| title_short | Cell-derived matrices |
| title_sort | cell derived matrices |
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